Ann Clin Microbiol 2020;23:21-31. Frequency of Mycobacterium tuberculosis Among M. tuberculosis Complex Strains Isolated from Clinical Specimen
| Primer type and target locus | Primer name | Nucleotide sequence (5′ → 3′) | Size, base pair |
|---|---|---|---|
| Rv0577 | Rv0577F | ATG CCC AAG AGA AGC GAA TAC AGG CAA | 786 |
| Rv0577R | CTA TTG CTG CGG TGC GGG CTT CAA | ||
| IS1561 (Rv3349c) | IS1561F | GCT GGG TGG GCC CTG GAA TAC GTG AAC TCT | 943 |
| IS1561R | AAC TGC TCA CCC TGG CCA CCA CCA TTG ACT | ||
| Rv1510 (RD4) | Rv1510F | GTG CGC TCC ACC CAA ATA GTT GC | 1,033 |
| Rv1510R | TGT CGA CCT GGG GCA CAA ATC AGT C | ||
| Rv1970 (RD7) | Rv1970F | GCG CAG CTG CCG GAT GTC AAC | 1,116 |
| Rv1970R | CGC CGG CAG CCT CAC GAA ATG | ||
| Rv3877/8 (RD1) | Rv3877/8F | CGA CGG GTC TGA CGG CCA AAC TCA TC | 999 |
| Rv3877/8R | CTT GCT CGG TGG CCG GTT TTT CAG C | ||
| Rv3120 (RD12) | Rv3120F | GTC GGC GAT AGA CCA TGA GTC CGT CTC CAT | 404 |
| Rv3120R | GCG AAA AGT GGG CGG ATG CCA GAA TAG T | ||
| Rv2073c (RD9) | Rv2073cF | TCG CCG CTG CCA GAT GAG TC | 600 |
| Rv2073cR | TTT GGG AGC CGC CGG TGG TGA TGA |
Abbreviation: MTB, Mycobacterium tuberculosis; IS, insertion sequence; RD, region of difference.