Ann Clin Microbiol 2020;23:21-31. Frequency of Mycobacterium tuberculosis Among M. tuberculosis Complex Strains Isolated from Clinical Specimen

Table 2. Primers used in spoligotyping and exact tandem repeat D sequencing
Primer type and target locusPrimer nameNucleotide sequence (5′ → 3′)Size, base pair
Spoligotyping   
DRDRaGGT TTT GGG TCT GAC GACVariable
 DRbCCG AGA GGG GAC GGA AAC 
ETR-D sequencing   
ETR-DETRDFGTT GAT CGA GGC CTA TCA CGVariable
 ETRDRGAA TAG GGC TTG GTC ACG TA 

Abbreviation: DR, direct repeat; ETRDF, exact tandem repeat D forward; ETRDR, exact tandem repeat D reverse.