Ann Clin Microbiol 2020;23:45-54. Role of Efflux Pump Gene adeIJK to Multidrug Resistance in Acinetobacter baumannii Clinical Isolates
| PCR | Gene | Primer | Sequences (5′ → 3′) | AT | References |
|---|---|---|---|---|---|
| Conventional PCR | gyrB Multiplex 1 | Sp4F | CAC GCC GTA AGA GTG CAT TA | 60°C | (11) |
| Sp4R | AAC GGA GCT TGT CAG GGT TA | ||||
| Sp2F | GTT CCT GAT CCG AAA TTC TCG | ||||
| blaOXA51-like | Oxa51-like F | TAA TGC TTT GAT CGG CCT TG | 55°C | (12) | |
| Oxa51-like R | TGG ATT GCA CTT CAT CTT GG | ||||
| Real-time RT-PCR | rpoB | rpoB F | CTC ACT ATG GTC GTG TTT GTC | 57°C | this study |
| rpoB R | CCA AGA AAC CGA AGT CAT TCG | ||||
| adeJ | adeJ F | CAA GTT ATT GCA TTC TAT TCA CCA G | 57°C | this study | |
| adeJ R | GAC CTG TAC CTC ACC AAC AC |
Abbreviation: AT, annealing temperature.