Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species

Table 2. Discrepant results among three real-time PCR assays and AFB culture in AFB smear positive specimens
Specimen type (No.)AdvanSureGenediaPowerChekNo. of specimensAFB culture results
Sputum (4), Bronchial aspirate (9)NTMNegativeNTM13NTM
Sputum (6), Bronchial aspirate (1)MTBMTBMTB7No growth
Sputum (1)MTBNegativeMTB1MTB
Bronchial aspirate (1)MTBMTBMTB, NTM1MTB
Sputum (1)NegativeNegativeNegative1MTB
Sputum (1)NegativeNegativeNegative1NTM (M. intracellulare + M. massiliense)
Bronchial aspirate (1)NegativeNegativeNTM1NTM (M. avium)
Bronchial aspirate (1)NTMNTMNTM, MTB1NTM (non-ID)

Abbreviation: AFB, acid-fast bacilli; MTB, Mycobacterium tuberculosis; NTM, nontuberculous mycobacteria, ID; identification.