Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species
| Parameter | AFB smear | AdvanSure | Genedia | PowerChek | |||||
|---|---|---|---|---|---|---|---|---|---|
| Result | 95% CI | Result | 95% CI | Result | 95% CI | ||||
| Sensitivity (%) | Positive | 90.9 | 70.8-98.9 | 54.6 | 32.2-75.6 | 95.5 | 77.2-99.9 | ||
| Negative | 40.0 | 5.3-85.3 | 0.0 | 0.0-52.2 | 60.0 | 14.7-94.7 | |||
| Total | 81.5 | 61.9-93.7 | 44.4 | 25.5-64.7 | 88.9 | 70.8-97.6 | |||
| Specificity (%) | Positive | 100.0 | 94.3-100.0 | 100.0 | 94.3-100.0 | 98.4 | 91.5-100.0 | ||
| Negative | 99.4 | 96.8-100.0 | 100.0 | 97.9-100.0 | 98.8 | 95.8-99.9 | |||
| Total | 99.6 | 97.6-100.0 | 100.0 | 98.4-100.0 | 98.7 | 96.3-99.7 | |||
| PPV (%) | Positive | 100.0 | 73.5-100.0 | 100.0 | 73.5-100.0 | 29.8 | 5.7-74.8 | ||
| Negative | 32.5 | 4.9-81.8 | Incomputable | Incomputable | 26.6 | 7.1-63.1 | |||
| Total | 57.3 | 15.9-90.5 | 100.0 | 73.5-100.0 | 32.8 | 13.6-60.3 | |||
| NPV (%) | Positive | 99.9 | 99.8-100.0 | 99.7 | 99.5-99.8 | 100.0 | 99.8-100.0 | ||
| Negative | 99.6 | 99.1-99.8 | 99.3 | 99.3-99.3 | 99.7 | 99.2-99.9 | |||
| Total | 99.9 | 99.7-99.9 | 99.6 | 99.5-99.7 | 99.9 | 99.8-100.0 | |||
| AUC | Positive | 0.96 | 0.89-0.99 | 0.77 | 0.66-0.86 | 0.97 | 0.89-0.99 | ||
| Negative | 0.91 | 0.86-0.94 | 0.72 | 0.66-0.78 | 0.94 | 0.90-0.96 | |||
| Total | 0.94 | 0.90-0.96 | 0.72 | 0.66-0.78 | 0.94 | 0.90-0.96 | |||
Abbreviation: PPV, positive predictive value; NPV, negative predictive value; AUC, area under the ROC curve; CI, confidence interval.