Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens
| Sensitivity % (No.) | Specificity % (No.) | PPV% | NPV% | |
|---|---|---|---|---|
| AdvanSure PCR | ||||
| MTC | 100.0 (45/45) | 83.8 (129/154*) | 32.1 | 100.0 |
| NTM | 64.0 (16/25) | 95.4 (166/174‡) | ||
| AdvanSure Plus PCR | ||||
| MTC | 100.0 (45†/45) | 83.8 (129/154*) | 32.1 | 100.0 |
| NTM | 68.0 (17/25) | 90.2 (157/174‡) | ||
| AFB staining | 40.0 (28/70) | 99.2 (128/129) |
*Included negative culture samples (n=129) and NTM positive culture samples (n=25).
†Included MTC & NTM co-positive samples (n=3) by AdvanSure plus PCR.
‡Included negative culture samples (n=129) and MTC positive culture samples (n=45).
§Calculated using Korean tuberculosis prevalence of 0.0768%.
Abbreviation: MTC, M. tuberculosis complex; NTM, nontuberculous mycobacteria; AdvanSure PCR, AdvanSure TB/NTM real-time PCR; AdvanSure plus PCR, AdvanSure plus TB/NTM real-time PCR.