Table 2. Initial interpretation criteria of SARS-CoV-2 molecular tests in the suspected patients

Ann Clin Microbiol 2020;23:165-171. Experience at Department of Laboratory Medicine during the COVID-19 Outbreak in Daegu

Table 2. Initial interpretation criteria of SARS-CoV-2 molecular tests in the suspected patients

Seegene	Ct values for:				Interpretation (1st Ed)	Interpretation (5th Ed)
Seegene	E	RdRp	N	IC	Interpretation (1st Ed)	Interpretation (5th Ed)
	<40	<33.5	<40	<40	Positive	Positive
	<40	<33.5	<40	≥40	Positive	Positive
	≥40	≥40	≥40	<40	Negative	Negative
	≥40	<33.5	<40	≥40	Repeat (Seegene)	Repeat after reextraction (Seegene)
	<40	≥33.5	<40
	<40	<33.5	≥40
	≥40	≥33.5	<40
	≥40	<33.5	≥40
	<40	≥33.5	≥40
	≥40	≥33.5	≥40
	≥40	<33.5	<40	<40	Repeat (Seegene+Kogene*)	Repeat after reextraction (Seegene +SD)
	<40	≥33.5	<40
	<40	<33.5	≥40
	≥40	≥33.5	<40
	≥40	<33.5	≥40
	<40	≥33.5	≥40
SD	Ct values for:				Interpretation
SD	E	ORF1ab		IC	Interpretation
	≤36	≤36		≤32	Positive
	>36	≤36		≤32	Same after repeat, Positive
	≤36	>36		≤32	Same after repeat, Negative
	>36	>36		≤32	Negative
	>36	>36		>32	Invalid, repeat

*When the test is repeated with Kogene, positive criteria for RdRp gene is ≤35.
Abbreviations: Ct, cycle of threshold; E, envelope gene; RdRp, RNA-dependent RNA polymerase gene; N, nucleocapsid gene; IC, internal control; ORF, open-reading frame gene.