Ann Clin Microbiol 2021;24:45-53. Evaluation of Two Commercial Kits for Rapid Detection and Typing of Carbapenemase in Carbapenem-Resistant Enterobacterales
| Primer target | Sequence (5′ to 3′) | References |
|---|---|---|
| KPC | 5′-GGC AGT CGG AGA CAA AAC C-3′ | [13] |
| 5′-CCC TCG AGC GCG AGT CTA-3′ | ||
| NDM | 5′-ACC GCC TGG ACC GAT GAC CA-3′ | [14] |
| 5′-GCC AAA GTT GGG CGC GGT TG-3′ | ||
| IMP | 5′-GGA ATA GAG TGG CTT AAT TCT C-3′ | [15] |
| 5′-CCA AAC CAC TAC GTT ATC T-3′ | ||
| 5′-CAT GGT TTG GTG GTT CTT GT-3′* | [17] | |
| 5′-ATA ATT TGG CGG ACT TTG GC-3′* | ||
| VIM | 5′-GAT GGT GTT TGG TCG CAT A-3′ | [15] |
| 5′-CGA ATG CGC AGC ACC AG-3′ | ||
| OXA-48 like | 5′-TTG GTG GCA TCG ATT ATC GG-3′ | [16] |
| 5′-GAG CAC TTC TTT TGT GAT GGC-3′ |
*Primer set for IMP-specific single-target PCR. Abbreviations: KPC, Klebsiella pneumoniae carbapenemase; NDM, New Delhi metallo-β-lactamase; IMP, imipenem-resistant Pseudomonas; VIM, Verona integron-encoded metallo-β-lactamase; OXA, oxacillinase.