Ann Clin Microbiol 2021;24:45-53. Evaluation of Two Commercial Kits for Rapid Detection and Typing of Carbapenemase in Carbapenem-Resistant Enterobacterales
| Genotyping results | No. of isolates | No. of positives (Carba5) | Sensitivity (%) / Specificity (%) | |||
|---|---|---|---|---|---|---|
| Carba5 | CPO | Carba5 | CPO | |||
| Carbapenemase-positives | 82 | 84 | 84 | 100 / 96.9 | 100 / 96.7 | |
| KPC | 24 | 24 | 24 | 100 / 100 | 100 / 100 | |
| NDM | 25 | 25 | 25 | 100 / 100 | 100 / 100 | |
| VIM | 22 | 22 | 27† | 100 / 100 | 100 / 98.4† | |
| IMP | 3 | 5 | 100 / 98.6 | |||
| OXA-48 like | 14 | 14 | 14 | 100 / 100 | 100 / 100 | |
| Carbapenemase-negatives | 65* | 63 | 63 | |||
*Two carbapenemase negative isolates were confirmed to be IMP carbapenemases later by re-testing conventional PCR and using the Carba-R assay. †The CPO assay was not designed to distinguish between VIM and IMP carbapenemases Abbreviations: PCR, polymerase chain reaction; KPC, Klebsiella pneumoniae carbapenemase; NDM, New Delhi metallo-β-lactamase; VIM, Verona integron-encoded metallo-β-lactamase; IMP, imipenemresistant Pseudomonas; OXA, oxacillinase.