Ann Clin Microbiol 2022;25:67-72. EUCAST v.12.0 review focused on changes
Table 3. Revised EUCAST v.12.0 clinical breakpoints
(A) Disk diffusion method: revised zone diameter breakpoints (mm), ATU
Organism Antimicrobial agent S ≥ R < ATU
P. aeruginosa Meropenem (indications other than meningitis) 20 14
Meropenem (meningitis) 20 20
Pseudomonasspp. Levofloxacin 50 18
S. epidermidisand S.lugdunensis Cefoxitin (screen only) 27 27 27
S. aureus Amikacin (15) (15)
Coagulase-negative staphylococci Amikacin (15) (15)
Tobramycin (20) (20)
Staphylococcus spp. Erythromycin (screen only) 21 21
Clindamycin 22 22
Tetracycline (screen only) 22 22
Tedizolid 20 20 19
Rimfampicin 26 26
Streptococcus group A,B,C, and G Tetracycline (screen only) 23 23
Rimfampicin 21 21
S. pneumoniae Ampicillin (indications other than meningitis) 22 19
Tetracycline (screen only) 25 25
Rimfampicin 22 22
H. influenzae Cefotaxime (meningitis) 27 27 25-27
Cefotriaxone (meningitis) 32 32 31-33
Tetracyline 25 25
M. catarrhalis Tetracyline 26 26
B. pseudomallei Tetracycline (screen only) 23 23
(B) Broth microdilution method: revised MIC breakpoints range (mg/L)
OrganismAntimicrobial agentS ≤R >
Pseudomonas spp.Levofloxacin0.0012
S. aureusDelafloxacin (community-acquired pneumonia)0.0160.016
Delafloxacin (skin and skin structure infections)0.250.25
Amikacin(16)(16)
Gentamicin(2)(2)
Tobramycin(2)(2)
Coagulase-negative staphylococciAmikacin(16)(16)
Gentamicin(2)(2)
Tobramycin(2)(2)
Staphylococcus spp.Azithromycin22
Erythromycin (screen only)11
Clindamycin0.250.25
Tetracycline (screen only)11
Rimfampicin0.060.06
Streptococcus group A,B,C, and GTetracycline (screen only)11
Rimfampicin0.060.06
S. pneumoniaeAmpicillin (indications other than meningitis)0.51
Tetracycline (screen only)11
Rimfampicin0.1250.125
H. influenzaeCefotaxime (meningitis)0.1250.125
Cefotriaxone (meningitis)0.1250.125
Tetracyline22
M. catarrhalisTetracyline22

The revised part is indicated in bold type. Breakpoints in brackets are based on epidemiological cut-off. Abbreviations: EUCAST, European Committee on Antimicrobial Susceptibility Testing; ATU, area of technical uncertainly; S, susceptible; R, resistance; MIC, minimal inhibitory concentration.