Ann Clin Microbiol 2023;26:19-27. Evaluation of two commercial kits for rapid pathogen identification directly from positive blood cultures by matrix-associated laser desorption/ionization time-of-flight mass spectrometry
Download table
Table 4. Six cases of polymicrobial infection have been directly identified
CaseGram stain MALDI Biotyper Sirius VITEK MS PRIMEConventional method
1st2nd SepsiTyperSepsiPrep SepsiTyperSepsiPrep
1(-) B(+) C, chain Enterobacter cloacae complexEnterobacter cloacae complex Enterobacter cloacae complexNo ID1. Enterobacter cloacae complex
2. Enterococcus faecium
3. Streptococcus parasanguinis
2(-) B(+) C, chain 1. Klebsiella pneumoniae
2. Enterococcus avium		Enterococcus avium Klebsiella pneumoniaeNo IDProteus mirabilis (covered plate)
3*(-) B(+) C, cluster 1. Klebsiella pneumoniae
2. Staphylococcus epidermidis		Staphylococcus epidermidis Klebsiella pneumoniaeKlebsiella pneumoniae1. Klebsiella pneumoniae
2. Staphylococcus epidermidis
4(-) B(+) C, chain Enterobacter cloacae complexEnterobacter cloacae complex Enterobacter cloacae complexEnterobacter cloacae complex1. Enterobacter cloacae complex
2. Staphylococcus epidermidis
5(-) B(+) C, chain Klebsiella pneumoniaeNo ID Klebsiella pneumoniaeNo ID1. Klebsiella pneumoniae
2. Streptococcus salivarius ssp.
Salivarius
6(+) B(+) C, chain Streptococcus mutansStreptococcus mutans Streptococcus mutansStreptococcus mutansStreptococcus gordonii

*Six polymicrobial cases were examined, and only one of them used the MALDI Biotyper Sirius and SepsiTyper kit to correctly identify both bacteria. Abbreviations: (-), negative; (+), positive; B, bacillus; C, coccus; ID, identification.