Ann Clin Microbiol 2025;28(3):16. Current status of mycobacterial identification in clinical laboratories in Korea
| Company | Vitek MS PRIME | MALDI Biotyper Sirius | IDSys LT® |
|---|---|---|---|
| bioMérieux | Bruker Daltonics | ASTA | |
| Panel | Disposable slide with 48 positions 192 isolates can be tested per run | Reuseable slide with 96 well | Disposable or reuseable 6 × 16 μFocus Plate |
| Criteria | 99.9%: identification | 2.300–3.000: Highly probable species identification | ≥ 140: Acceptable species identification |
| 99.8%–90.0%: identification | 2.000–2.299: Secure genus identification, probable species identification | < 140, ≥ 130: Need for confirmation | |
| 89.9%–85.0%: identification | 1.700–1.999: Probable genus identification | < 130: Not reliable identification | |
| 84.9%–70.0%: no identification | 0.000–1.699: Not reliable identification | ||
| Database for mycobacteria | VITEK MS V3.2 MYCOBACTERIUM DATABASE (IVD) SARAMIS Knowledge Base V4.16 (RUO) | MBT Mycobacteria IVD software | MicroIDSys – MycoDB v2.0 |
| IVD: 39 FDA claimed and unclaimed species and subspecies including M. tuberculosis complex, M. fortuitum group, M. abscessus, M. intracellulare SARAMIS: more than 60 mycobacteria species | 182 species entries including M. abscessus, M. intracellulare, M. fortuitum, M. tuberculosis | 71 mycobacterial species including M. fortuitum, M. abscessus, M. kansasii, M. avium, M. intracellulare, M. chelonae, M. gordonae, M. szulgai | |
| Pretreatment step | 1) 70% ethanol and 0.5 mm glass bead 2) 70% formic acid and 100% acetonitrile 3) VITEK MS-CHCA matrix | 1) Inactivation reagent 2) Acetonitrile and formic acid mix 3) CHCA matrix | 1) Silica beads, Sodium dodecyl sulfate (SDS), 70% ethanol 2) Acetonitrile and formic acid 3) CHCA matrix |
Abbreviations: MALDI-TOF MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; FDA, the U.S. Food and Drug Administration.