Ann Clin Microbiol 2025;29(1):1. Performance of C. Diff Quik Chek Complete and RIDASCREEN immunoassays and lack of Ct value concordance between Allplex GI-Bacteria(I) and Xpert Clostridioides difficile assays: a diagnostic accuracy study
| Sensitivity (95% CI) | Specificity (95% CI) | Positive likelihood ratio (95% CI) | Negative likelihood ratio (95% CI) | AUC (95% CI, P) | PPV (95% CI) | NPV (95% CI) | Accuracy (95% CI) | |
|---|---|---|---|---|---|---|---|---|
| QCC GDH | 100.0% (90.3–100.0) | 92.4% (86.7–96.1) | 13.1 (7.4–23.1) | 0.0 | 0.959 (0.918–0.983, <0.001) | 76.6% (65.0–85.2) | 100.0% (97.3–100.0) | 93.9% (89.3–96.9) |
| RIDA GDH | 94.4% (81.3–99.3) | 88.2% (81.8–93.0) | 8.0 (5.1–12.6) | 0.1 (0.0–0.2) | 0.927 (0.879–0.960, <0.001) | 66.7% (56.0–75.9) | 98.4% (94.3–99.6) | 89.4% (84.0–93.5) |
| QCC toxin A/B | 51.4% (34.0–68.6) | 99.3% (96.2–100.0) | 74.6 (10.3–539.9) | 0.5 (0.4–0.7) | 0.736 (0.665–0.799, <0.001) | 94.7% (71.3–99.2) | 89.4% (85.8–92.2) | 90.0% (84.7–94.0) |
| RIDA toxin A/B | 28.6% (14.6–46.3) | 100.0% (97.5–100.0) | – | 0.7 (0.6–0.9) | 0.643 (0.568–0.713, <0.001) | 100.0% (69.2–100.0) | 85.3% (82.5–87.7) | 86.1% (80.2–90.8) |
Abbreviations: GDH, glutamate dehydrogenase; CI, confidence interval; AUC, area under the curve; PPV, positive predictive value; NPV, negative predictive value; QCC, C. Diff Quik Chek Complete; RIDA, RIDASCREEN C. difficile assay.