Table 1. Primers and polymerase chain reaction conditions used in this study

Ann Clin Microbiol 2026;29(3):14. Molecular epidemiological characteristics of monophasic Salmonella enterica serovar Typhimurium variants in Korea

Table 1. Primers and polymerase chain reaction conditions used in this study

Targets	Name	Primer sequence (5′–3′)	Size (bp)	Cycling profile
Intergenic region of fliA–fliB	FFLIB	CTG GCG ACG ATC TGT CGA TG	964	(30 cycles) 94℃, 30 s/54℃, 30 s/72℃, 90 s
Intergenic region of fliA–fliB	RFLIA	GCG GTA TAC AGT GAA TTC AC	964	(30 cycles) 94℃, 30 s/54℃, 30 s/72℃, 90 s
fljB	Sense-59	CAA CAA CAA CCT GCA GCG TGT GCG	1389	(30 cycles) 94℃, 30 s/62℃, 30 s/72℃, 30 s
fljB	Antisense-83	GCC ATA TTT CAG CCT CTC GCC CG	1389	(30 cycles) 94℃, 30 s/62℃, 30 s/72℃, 30 s
fliC	Sense-60	ACT CAG GCT TCC CGT AAC GC	550	(30 cycles) 94℃, 40 s/58℃, 20 s/72℃, 20 s
fliC	Antisense-i	ATA GCC ATC TTT ACC AGT TCC	550	(30 cycles) 94℃, 40 s/58℃, 20 s/72℃, 20 s
STM2757	STM2757-F	AAC CGT ACA GGG TTT ATA CGC C	91	(35 cycles) 95℃, 15 s/60℃, 1 min/72℃, 30 s
STM2757	STM2757-R	TTA TCG TGC CGC CGA ATT ATG G	91	(35 cycles) 95℃, 15 s/60℃, 1 min/72℃, 30 s
mdh	mdh-F	TGC CAA CGG AAG TTG AAG TG	260	(30 cycles) 94℃, 30 s/55℃, 30 s/72℃, 30 s
mdh	mdh-R	CGC ATT CCA CCA CGC CCT TC	260	(30 cycles) 94℃, 30 s/55℃, 30 s/72℃, 30 s
fljA	fljA-F	TCC GAA GCC AGA ATC AAA TTT TCC	105	(35 cycles) 95℃, 15 s/60℃, 1 min/72℃, 30 s
fljA	fljA-R	TAC GTT TTA ATG ATA TCC CTG TTC G	105	(35 cycles) 95℃, 15 s/60℃, 1 min/72℃, 30 s
hin	hin-F	CGC CCC GGC CTG AAA CGA	334	(35 cycles) 95℃, 15 s/60℃, 1 min/72℃, 30 s
hin	hin-R	CGA CTA ATC TGT TCC TGT TCA TGT T	334	(35 cycles) 95℃, 15 s/60℃, 1 min/72℃, 30 s