Ann Clin Microbiol 2020;23:21-31. Frequency of Mycobacterium tuberculosis Among M. tuberculosis Complex Strains Isolated from Clinical Specimen
| Primer type and target locus | Primer name | Nucleotide sequence (5′ → 3′) | Size, base pair |
|---|---|---|---|
| Spoligotyping | |||
| DR | DRa | GGT TTT GGG TCT GAC GAC | Variable |
| DRb | CCG AGA GGG GAC GGA AAC | ||
| ETR-D sequencing | |||
| ETR-D | ETRDF | GTT GAT CGA GGC CTA TCA CG | Variable |
| ETRDR | GAA TAG GGC TTG GTC ACG TA |
Abbreviation: DR, direct repeat; ETRDF, exact tandem repeat D forward; ETRDR, exact tandem repeat D reverse.