Ann Clin Microbiol 2024;27(4):245-255. Current nonculture-based diagnosis of candidemia

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Table 3. Characteristics of several molecular diagnostics for candidemia approved by Conformité Européenne/ In Vitro Diagnostic or Food and Drug Administration
Commercial kit Manufacturer Detectable Candida species Needs of nucleic acid extraction Principle Available specimen Assay time Reference
eplex® BCID FP Panela) GenMark Dx C. albicans, C. dubliniensis, C. famata, C. glabrata, C. guilliermondii, C. kefyr, P. kudriavzevii, C. lusitaniae, C. parapsilosis, C. tropicalis, C. auris yes Multiplex PCR Blood culture positive sample 90 min [33]
CandID OLM Diagnostics C. albicans, C. dubliniensis, C. glabrata, P. kudriavzevii, C. parapsilosis, C. tropicalis yes Multiplex qPCR Blood, synthetic bronchial aspirate lavage 4 h [36]
Fungiplex Candida Bruker Daltonics C. glabrata, Candida species (C. albicans, C. dubliniensis, C. parapsilosis, C. tropicalis), P. kudriavzevii yes Multiplex real-time PCR Blood < 2 h [37]
SeptiFast Test kit Roche Diagnostics C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, P. kudriavzevii yes Multiplex real-time PCR Blood, sterile fluid, tissue, swab 6 h [37]
Magicplex Sepsis Seegene C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, P. kudriavzevii yes Multiplex PCR Blood 6 h [47]
FilmArray BCID Panel bioMérieux C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, P. kudriavzevii no Multiplex PCR Blood culture positive sample 60 min [43]
FilmArray BCID2 Panel bioMérieux C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, P. kudriavzevii, C. auris, Cryptococcus neoformans/C. gattii no Multiplex PCR Blood culture positive sample 60 min [44]
T2 Candida Panel T2 Biosystems C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, P. kudriavzevii no Multiplex PCR followed by automated T2MR-based detection Blood < 5 h [45,46]

a)The ePlex® BCID assay was rebranded as Cobas® eplex Blood Culture Identification Panels (Roche Diagnostics, Basel, Switzerland) in 2021.
Abbreviations: qPCR, quantitative PCR; T2MR, T2 magnetic resonance.