Ann Clin Microbiol 2025;28(1):3. Mock communities to assess biases in next-generation sequencing of bacterial species representation

Download table
Table 2. Results of multiple regression analysis of mock communities
Constant V3V4 %GC 16S rRNA copy No. Genome size Gram-stain R-squared Adjusted R-squared
DNA mock
 Coefficient 27.143 -0.397 0.607 -9.914E-07 0.008 0.650 0.542
 Std. Error 7.482 0.139 0.179 0 1.880
 t-Statistic 3.628 -2.858 3.387 -1.707 0.004
 P-value 0.003 0.013 0.005 0.112 0.997
Plasmid mock
 Coefficient 27.062 -0.401 N/A N/A N/A 0.256 0.209
 Std. Error 9.186 0.171 N/A N/A N/A
 t-Statistic 2.946 -2.346 N/A N/A N/A
 P-value 0.009 0.032 N/A N/A N/A
PCR product mock
 Coefficient 28.248 -0.423 N/A N/A N/A 0.401 0.363
 Std. Error 6.951 0.129 N/A N/A N/A
 t-Statistic 4.064 -3.271 N/A N/A N/A
 P-value 0.001 0.005 N/A N/A N/A

Abbreviations: PCR, polymerase chain reaction; N/A, not applicable.