Fig. 3. Bacterial RNA isolation workflow. Mechanical lysis was performed using a MagNA lyser (Roche). Human keratinocytes were lysed with 1.4 mm silica beads (Qbiogene) in RLT lysis buffer (RNeasy Fibrous Tissue Mini Kit; Qiagen), and the human RNA fraction was removed by centrifugation. The pellets were then lysed with 0.1 mm silica beads (Qbiogene) in RLT lysis buffer and centrifuged to obtain the bacterial RNA fraction. Figure cited from [29].