Perspective Bosung Park, Mi-Na Kim Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea Correspondence to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2025;28(2):9. https://doi.org/10.5145/ACM.2025.28.2.3Received on 8 May 2025, Revised on 22 May 2025, Accepted on 26 May 2025, Published on 16 June 2025.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article which is freely available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/). Diagnostic stewardship aims to improve patient care quality by optimizing diagnostic test utilization, reducing unnecessary testing, and enhancing cost-effectiveness [1,2]. In Korea, diagnostic practices are in the early stages of developing a stewardship program. Although not exclusive to clinical microbiology, diagnostic stewardship programs (DSPs) have gained prominence owing to the global challenge of antimicrobial resistance, aligning with the expansion of antimicrobial stewardship programs (ASPs) [3]. This commentary examines the current status and perspectives of DSPs in

Case report Bosung Park1, Ho Seok Chung2, Eun Jeong Won1, Heungsup Sung1, Mi-Na Kim1 1Department of Laboratory Medicine, 2Department of Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea Corresponding to Eun Jeong Won, E-mail: ejwon@amc.seoul.kr Ann Clin Microbiol 2024;27(2):149-153. https://doi.org/10.5145/ACM.2024.27.2.9Received on 19 April 2024, Revised on 20 May 2024, Accepted on 20 May 2024, Published on 20 June 2024.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article which is freely available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/). Abstract Acanthamoeba species are ubiquitous, free-living organisms found in the environment. They can cause a sight-threatening cornea disease, termed Acanthamoeba keratitis, and are often misdiagnosed, causing delayed administration of the correct treatment. Herein, we report a case of Acanthamoeba keratitis diagnosed without culture. A 12-year-old girl with a history of wearing contact lenses presented with complaints of pain, irritation, and hyperemia in the left eye. Corneal