Original article PDF Kyoung Ho Roh1, Chang-Ki Kim2, Jong Hwa Yum3, Dongeun Yong4,5, Seok Hoon Jeong4,5, Chae Seung Lim1, Chang Kyu Lee1, Yunjung Cho1, Kyungwon Lee4,5, Yunsop Chong4,5 1Department of Laboratory Medicine, Korea University College of Medicine, 2Korean Institute of Tuberculosis, Seoul, 3Department of Clinical Laboratory Science, Dongeui University, Busan, 4Department of Laboratory Medicine and 5Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2010;13(1):27-33. https://doi.org/10.5145/KJCM.2010.13.1.27Copyright © Korean Society of Clinical Microbiology. Abstract Background: Increasing numbers of Acinetobacter spp. resistant to multiple drugs, including carbapenem, has been a serious problem. The aims of this study were to determine carbapenem resistance patterns and mechanisms, as well as to study the molecular epidemiology of Acinetobacter spp. Methods: Clinical isolates of Acinetobacter spp. were collected from May to November in 2006. Antimicrobial susceptibility testing was performed using CLSI disk diffusion and

Original article PDF Hyejin Lee1, Chang Kyu Lee1, Myung-Hyun Nam1, Kyoung Ho Roh1, Soo-Young Yoon1, Chae Seung Lim1, Yunjung Cho1, Young Kee Kim1, Kap No Lee1, Young Yoo2 Departments of 1Laboratory Medicine, and 2Pediatrics, Korea University College of Medicine, Seoul, Korea Corresponding to Chang Kyu Lee, E-mail: cklee@korea.ac.kr Ann Clin Microbiol 2012;15(2):54-59. https://doi.org/10.5145/KJCM.2012.15.2.54Copyright © Korean Society of Clinical Microbiology. Abstract Background: Parainfluenza virus (PIV) is a significant cause of acute respiratory infections. Epidemiological information on PIV infection could be very helpful for patient management. The aim of this study was to investigate the epidemiology of PIV infection in Seoul and a neighboring area with regard to PIV type. Methods: The diagnosis of PIV infection was made by virus isolation. The R-mix Too cell system (Diagnostic Hybrids, Inc., Athens, OH, USA) and D3 Ultra DFA Respiratory Virus Screening & ID kits (Diagnostic Hybrids, Inc.) were used for virus culture and identification.

Case report PDF Young Jin Ko1,2, Soo-Young Yoon2, Chae Seung Lim2, Chang Kyu Lee2 1Department of Laboratory Medicine, Chosun University Hospital, Gwangju, 2Department of Laboratory Medicine, Korea University College of Medicine, Seoul, Korea Corresponding to Young Jin Ko, E-mail: md.seraph@gmail.com Ann Clin Microbiol 2019;22(4):105-109. https://doi.org/10.5145/ACM.2019.22.4.105Copyright © Korean Society of Clinical Microbiology. Abstract We report a case of cellulitis caused by a novel Cupriavidus species identified using whole-genome sequence analysis. Subcutaneous tissue biopsies from the left lower leg of a 67-year-old man who suffered from cellulitis were cultured. Round, convex, gray and non-hemolytic colonies were recovered after 72-h incubation. 16S rRNA sequence analysis showed 98.6% similarity with Cupriavidus basilensis DSM 11853(T) in the NCBI database and 99.9% similarity with C. basilensis KF708 in the EzBioCloud database. Genomic analysis using the MiSeq platform (Illumina, USA) and the TrueBac ID database (ChunLab, Korea) revealed that the average nucleotide identity (ANI) of this strain

Original article Sang-wook Kim1*, Young-Hee Park2*, Young Jin Ko1,3, Yoon Ho Kim2, Chang Hyun Kim2, Chae Seung Lim1 Department of Laboratory Medicine, 1Korea University College of Medicine, Seoul, 2Korea University Medical Center Guro Hospital, Seoul, 3Department of Laboratory Medicine, College of Medicine, Chosun University, Gwangju, Korea *These authors contributed equally to this work. Corresponding to Young Jin Ko, E-mail: yjko@chosun.ac.kr Ann Clin Microbiol 2020;23(2):93-104. https://doi.org/10.5145/ACM.2020.23.2.6Received on 19 November 2019, Revised on 29 December 2019, Accepted on 29 December 2019, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The disease burden caused by Mycobacterium tuberculosis (MTB) complex continues to decrease in most countries. However, the diseases caused by the nontuberculous mycobacteria (NTM) become a public health problem. This study aimed to compare the diagnostic accuracy of three real-time PCR assays: AdvanSure TB/NTM real-time PCR kit (AdvanSure; LG Chem., Korea), Genedia MTB/NTM detection kit (Genedia; Green Cross