Perspective Dongeun Yong1,2 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Microbiotix, Inc., Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2024;27(3):179-183. https://doi.org/10.5145/ACM.2024.27.3.5Received on 28 August 2024, Revised on 8 September 2024, Accepted on 9 September 2024, Published on 20 September 2024.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article which is freely available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/). Introduction The rise of multidrug-resistant organisms is a significant global health threat, projected to cause up to 10 million deaths annually by 2050, surpassing cancer as the leading cause of mortality [1]. Traditional antibiotic development has not kept pace with this threat, necessitating innovative approaches to combat resistant pathogens. Bacteriophage therapy, which leverages viruses to specifically target and destroy bacteria, has emerged as a promising alternative. This letter outlines the key

Original article Justin Mugiraneza1, Mijung Kwon2, Daewon Kim2, Sang-Guk Lee2, Hyukmin Lee2, Dongeun Yong2 1Department of Global Health Security, Yonsei University Graduate school of Public Health, Seoul, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2020;23(3):125-134. https://doi.org/10.5145/ACM.2020.23.3.2Received on 28 January 2020, Revised on 27 March 2020, Accepted on 27 March 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The rapid antimicrobial susceptibility testing (AST) performed on urine samples would guide the adequate choice of antibiotics for obtaining better treatment outcomes in patients. Our study aimed to evaluate the performance of the modified-EUCAST (European Committee on Antimicrobial Susceptibility Testing) rapid direct AST on urine samples. Methods: From >2,000 urine samples, a total of 128 urine samples containing bacterial counts of ≥2 × 104 CFU/mL with a uniform

Original article PDF Myungsook Kim*,** Sunhee Kim* Giyeon Kang,* Dongeun Yong*,**, Kyungwon Lee*,**, Yunsop Chong,*,** and Shin Moo Kim*** Department of Laboratory Medicine,* Research Institute of Bacterial Resistance,** Yonsei University College of Medicine, Seoul; Department of Clinical Pathology, Wonkwang Health Science College, Iksan, Chonbuk, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2002;5(2):129-136.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid species identification of enterococci is necessary for optimal treatment of infected patients as they are frequently resistant to various antimicrobial agents. Minimal identification scheme is necessary to cut the laboratory cost. In this study, a minimal identification system was modified to expand the identifiable species. Methods:Performance of MGP test was compared to that of MIO motility test. Colonies on blood agar were used to inoculate primary identification media: SFA, BEAA, mannitol agar, tellurite agar, sorbose agar and MGP agar, which were prepared in biplates. Pigment production

Original article PDF Dongeun Yong, M.D.,* Hyuk Min Lee, M.D.,* Jong Ha Ryu, M.D.,* Kyung Ho Roh, M.D.,* Won Ho Kim, M.D.,**,*** Kyungwon Lee, M.D.,*,*** and Yunsop Chong, M.D. Department of Laboratory Medicine, Research Institute of Bacterial Resistance,* Department of Internal Medicine,** and Brain Korea 21 Project for Medical Sciences,*** Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2002;5(2):124-128.Copyright © Korean Society of Clinical Microbiology. Abstract Background: C. difficile-associated diarrhea (CDAD), the most frequently identified cause of nosocomial diarrhea, results from the overgrowth of cytotoxin (toxin B)-producing strains. The aim of this study was to evaluate the quantitative culture of Clostridium difficile to improve the laboratory diagnosis of CDAD. Methods: The quantitative culture and cytotoxin gene results were evaluated based on the findings of colonoscopy and/or histology of the biopsy specimens. Results: Among the 402 specimens with cytotoxin-positive isolates, 301 (74.9%) contained ≥106 CFU/mL of C. difficile.

Original article PDF Seong Geun Hong1, Sunjoo Kim2, Seok Hoon Jeong3, Chulhun L. Chang4, Sung Ran Cho5, Ji Young Ahn5, Jong Hee Shin6, Hye Soo Lee7, Won Keun Song8, Young Uh9, Jong Hwa Yum10, Dongeun Yong10, Kyungwon Lee10, Yunsop Chong10 Department of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Gyeongsang Nat. Univ. School of Med.2, Coll. of Med. Kosin Univ.3, Pusan Nat. Univ. Coll. of Med.4, Sooncheonhyang Univ. Coll. of Med.5, Chonnam Nat. Univ. Med. School6, Chonbuk Univ. Med. School7, Hanllym Univ. Coll. of Med.8, Yonsei Univ. Wonju Coll. of Med.9, Yonsei Univ. Coll. of Med.10 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(2):149-155.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Increase in extended-spectrum β-lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae have been reported in Korea. The aim of this study was to determine the nationwide prevalence of ESBL-producing E. coli and K. pneumoniae, and to investigate the types of ESBLs. Methods：A total of 2,221 E. coli and

Original article PDF Wee Gyo Lee, Suck Ho Lee*, Sun Min Lee**, Ji Young Huh, Dongeun Yong***, Kyungwon Lee***, Wan Shik Shin**** and Dong Gun Lee**** Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Division of Gastroenterology*, Department of Internal Medicine, Sooncheonhyang University Cheonan Hospital, Cheonan, Department of Laboratory Medicine, Bestian Medical Center**, Yonsei University School of Medicine***, Division of Infectious Disease****, Department of Internal Medicine, Catholic University, Seoul, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann Clin Microbiol 2003;6(2):119-125.Copyright © Korean Society of Clinical Microbiology. Abstract Background : The widespread dissemination of Tn1546 has been attributed to transposition into plasmid or transferable elements. The transposition has been achieved through the activity of insertion sequences. Genetic diversity in Tn1546 includes integration of IS elements such as IS1216V, IS1251, IS1476 and IS1542. We investigated molecular typing and the distribution of insertion sequences in vanA-containing Enterococcus faecium isolated from patients in a teaching hospital. Methods

Original article PDF Ji Young Huh, Sun Min Lee*, Suck Ho Lee**, Wee Gyo Lee, Dongeun Yong***, Kyungwon Lee***, Wan Shik Shin**** and Dong Gun Lee**** Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Bestian Medical Center*, Seoul, Division of Gastroenterology**, Department of Internal Medicine, Sooncheonhyang University Cheonan Hospital, Cheonan, Department o f Laboratory Medicine***, Yonsei University School of Medicine, Division of Infectious Disease****, Department of Internal Medicine, Catholic University, Seoul, Korea Corresponding to Ji Young Huh, E-mail: heidi2030@hanmail.net Ann Clin Microbiol 2003;6(2):114-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Vancomycin-resistant enterococci (VRE) have been increasingly isolated worldwide as a nosocomial pathogen. In Korea, because avoparcin has been used as a growth promoter in animal feed, vanA-containing enterococci have been found in animals. The aim of this study is to understand the epidimiology of VRE isolated from chicken of diverse geographic areas.  Methods : Thirty eight isolates