Original article PDF Dae-Gu Son*, Eun-Hee Kwon, Hye-Gyung Bae, Woon-Bo Heo, Nan-Young Lee,Dong-Il Won, Kyung-Eun Song, Jang-Soo Suh, Won-Kil Lee Department of Plastic Surgery, Keimyung University, DongSan Medical Center*, Department of Clinical Pathology, Kyungpook National University, School of Medicine Taegu, Korea Corresponding to Won-Kil Lee, E-mail: leewk@knu.ac.kr Ann Clin Microbiol 2002;5(2):84-96.Copyright © Korean Society of Clinical Microbiology. Abstract Bakground: In recent years, knowledge of bacterial resistance to antimicobials has expanded in important ways. Availability of an increasing number of antibiotics allows more precise individualization of resistance phenotypes and recording susceptibility results as patterns or phenotypes is valuable for both surveillance and patient care. If the patterns of resistance to panels of related antimicrobials are considered the underlying mechanisms can often be inferred. And the inferred mechanisms make the clinician to be advised to use alternative treatment. Interpretation of resistance phenotypes is based on the comparison of clinical isolates with prototype

Original article PDF Won-Kil Lee, Eun-Hee Kwon, Hye-Gyung Bae, Jang Soo Suh, Kyung Eun Song, Nan Young Lee, Dong Il Won, Jung Bum Lee* Department of Clinical Pathology, Family Medicine*, Kyungpook National University, School of Medicine, Taegu Corresponding to Won Kil Lee, E-mail: leewk@knu.ac.kr Ann Clin Microbiol 2003;6(1):81-87.Copyright © Korean Society of Clinical Microbiology. Abstract Background: There is growing evidence linking infection with Chlamydophila pneumoniae with vascular diseases, such as atherosclerosis and myocardial infarction. However, the data remain inconclusive and the clinical importance of C. pneumoniae as vasculopathic is unclear. So, we intend to detect C. pneumoniae in acute myocardial infarction patients by microimmunofluorescence (mIF) and polymerase chain reaction (PCR). Methods:Blood and peripheral mononuclear cells (PMNCs) of 24 myocardial infarction patients and 100 normal controls were collected. Serum were used in mIF and PMNCs in PCR. PMNC sample were tested for C. pneumoniae by ‘touchdown’nested PCR. The first round PCR amplified DNA from both C. pneumoniae and Chlamydophila psittaci, while the

Original article PDF Jongyoun Yi1, Jae-Kyoo Lee1, Eun-Hee Chung2, Dong Hee Cho3, and Eui-Chong Kim1 Department of Laboratory Medicine, Seoul National University College of Medicine1, Department of Pediatrics, Dankook University College of Medicine2, Department of Laboratory Medicine, Sungkyunkwan University School of Medicine3, Republic of Korea Corresponding to Eui-Chong Kim, E-mail: euichong@snu.ac.kr Ann Clin Microbiol 2004;7(1):55-58.Copyright © Korean Society of Clinical Microbiology. Abstract Background : We investigated the causative agents of hemorrhagic diarrhea which occurred in newborn babies in a hospital nursery in July, 2002. Rotavirus was not confirmed as the cause because only a few patients were positive for rotavirus test while most others with hemorrhagic diarrhea were negative. Therefore, patients with bloody stool were tested for Salmonella, Shigella, rotavirus, adenovirus, enterovirus, astrovirus, and enterohemorrhagic Escherichia coli (EHEC). Methods : Bloody stools from 12 newborns with hemorrhagic diarrhea were tested. Polymerase chain reaction (PCR) of shiga-toxin gene was performed for EHEC. Rotavirus and adenovirus