Original article Ji-Ae Choi1, Choon-Mee Kim2, Sook-Jin Jang3, Seong-Sik Cho4, Chul-Ho Jang5, Young-Jin Ko3, Seong-Ho Kang3, Geon Park3 1Division of Antimicrobial Resistance, Center for Infectious Diseases Research, Korea National Institute of Health, KCDC, Cheongju, 2Premedical Science, 3Department of Laboratory Medicine, College of Medicine, 4Department of Laboratory Medicine, Chosun University Hospital, Gwangju, 5Department of Otolaryngology, Chonnam National University Medical School, Gwangju, Republic of Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2020;23(1):45-54. https://doi.org/10.5145/ACM.2020.23.1.45Received on 20 September 2019, Revised on 18 November 2019, Accepted on 1 December 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The emergence of multidrug-resistant Acinetobacter baumannii as a nosocomial pathogen is one of the major public health problems. The aim of this study was to evaluate the role of an efflux pump gene adeJ for the multidrug resistance of A. baumannii clinical isolates. Methods: Two groups (MDRAB and SAB)

Original article Ung-Jun Kim1, Choon-Mee Kim2, Sook-Jin Jang1, Seul-Bi Lee1, Seong-Sik Cho1, Seok-Hoon Jeong3, Young-Jin Ko1, Seong-Ho Kang1, Geon Park1, Dong-Min Kim4, Na-Ra Yoon4, Young-Joon Ahn5, Dong-hoon Lim6, Joong-Ki Kook7 1Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine, Seoul2Department of Parasitology and Tropical Medicine, Gyeongsang National University College of Medicine, Jinju;3MediCheck Research Institute, Korea Association of Health Promotion, Seoul, Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2020;23(1):11-20. https://doi.org/10.5145/ACM.2020.23.1.11Received on 29 May 2019, Revised on 11 September 2019, Accepted on 11 September 2019, Published on 20 June 2024.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Acinetobacter baumannii infection is a significant health problem worldwide due to increased drug resistance. The limited antimicrobial alternatives for the treatment of severe infections by multidrug-resistant A. baumannii (MDRAB) make the search for other therapeutic options more urgent. Linalool, the major oil compound in Coriandrum sativum, was recently

Original article PDF Sook-Jin Jang1,2, Jin Hee Kim1, Young Sook Kim3, Jong Hee Shin4, Geon Park1, Bidur Prasad Chaulagain1,2, Dae Soo Moon1, Young Jin Park1 Department of Laboratory Medicine1, Research Center for Resistant Cells2, and Department of Diagnostic Radiology3, Chosun University Medical School; and Department of Laboratory Medicine4, Chonnam University Medical School, Gwangju, Korea Corresponding to Young Sook Kim, E-mail: yshkim@chosun.ac.kr Ann Clin Microbiol 2006;9(1):64-70.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid detection of pathogens in blood is important in patient management, because the mortality rate associated with bloodstream infections is very high. We evaluated the efficiency of a 16S rDNA PCR assay for the detection of various pathogens in blood culture broth in a clinical laboratory. Methods: 16S rDNA PCR was performed on 221 blood culture bottles consisting of 99 culturepositive and 122 culture-negative samples. The results were compared with conventional culture methods. We also compared the

Original article PDF Hu-Lin Han1, Sook-Jin Jang1,2, Geon Park2, Jong-Hee Shin3, Sung-Heui Shin1, Young-Lae Moon4, Dae-Soo Moon2, Young-Jin Park2 1Research Center for Resistant Cells, Departments of 2Laboratory Medicine and 4Orthopaedic Surgery, College of Medicine, Chosun University, 3Department of Laboratory Medicine, College of Medicine, Chonnam National University, Gwangju, Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2007;10(2):96-101.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the usefulness of a newly developed molecular typing method of infrequent restriction site polymerase chain reaction (IRS-PCR) as an epidemiological DNA fingerprinting tool for Candida tropicalis. Methods: Thirty-two strains of C. tropicalis comprising eight sporadic strains and 24 clonal strains belonging to six clones, of which clonal type were previously confirmed by pulsed-field gel electrophoresis (PFGE), were tested by IRS-PCR to evaluate the usefulness of this technique. Twenty strains of Candida species, including C. glabrata, C. krusei, C. albicans, and C. parapsilosis, were also tested to assess the ability of IRS-PCR to discriminate among

Original article PDF Geon Park, M.D.1, Sook-Jin Jang, M.D.1,2, Ho-Jong Jeon, M.D.3, Seong-Hwan Kim, M.D.4, Mi-Ja Lee, M.D.3, Jin-Hee Kim, M.S.1, Sung-Heui Shin, M.D.2, Bidur Prasad Chaulagain, M.S.1,2, Dong-Min Kim, M.D.5, Dae-Soo Moon, M.D.1, Young-Jin Park, M.D.1 Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Sook Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2007;10(2):84-89.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In hepatocellular carcinoma (HCC), the frequency of p53 mutation and the association with hepatitis B virus (HBV) infection varies with geographic locations and risk factors. The aim of this study was to determine the frequency of codon 249 mutation of p53, p53 overexpression, and HBV DNA positivity and to observe the relationship between them in Korean HCC. Methods: We analyzed overexpression of p53 in hepatoma tissue from 17 HCC patients by immunohistochemistry (IHC), specific mutations at the third base position of

Original article PDF Hye Ryong Oh1, Sook Jin Jang1,2, Feng Nan Yu1,2, Geon Park1, Xue Min Li2, Sung Heui Shin2, Won Yong Kim3, Dae Soo Moon1, Young Jin Park1 1Departments of Laboratory Medicine, 2Research Center for Resistant Cells, Chosun University Medical School, Gwangju; 3Department of Microbiology, Chungang University Medical School, Seoul, Korea Corresponding to Sook Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2007;10(2):128-134.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The incidence of infections with imipenem-resistant Acinetobacter baumannii (IRAB) and Pseudomonas aeruginosa (IRPA) is increasing worldwide, and recent molecular studies indicate that the prevalence of carbapenemases is increasing in various parts of the world. However, few long-term longitudinal studies have assessed the prevalence of IRAB- and IRPA-derived carbapenemases and integrases in a hospital setting in Korea. Methods: The carbapenemase genes (blaOXA-23, blaOXA-24, blaOXA-58, blaIMP-1, blaVIM-2, blaSIM-1, blaSPM-1) and integrase genes (intI1, intI2, intI3) produced by 46 IRAB strains and 51 IRPA strains collected at Chosun University Hospital between 2003 and 2006

Case report PDF Hye Ryong Oh,1 Young Sook Kim2, Sook Jin Jang1,3, Xue Min Li3, Won Yong Kim4, Geon Park1, Dae Soo Moon1, Young Jin Park1 Departments of 1Laboratory Medicine, 2Radiology, and 3Research Center for Resistant Cells, Chosun University Medical School, Gwangju, 4Department of Microbiology, Chungang University Medical, Seoul, Korea Corresponding to Young Sook Kim, E-mail: yshkim@chosun.ac.kr Ann Clin Microbiol 2008;11(2):136-140.Copyright © Korean Society of Clinical Microbiology. Abstract Nocardia cyriacigeorgica is an aerobic gram-positive rod that has mostly been reported as an opportunistic pathogen. Since molecular methodologies were introduced to identify species, infections caused by N. cyriacigeorgica have been reported. The patient was a 51-year-old woman with aplastic anemia, systemic lupus erythematosus, and disseminated tuberculosis, who was admitted to Chosun University Hospital with a history of fever and productive cough. During her hospitalization, sputum cultures were taken and a bacterium suspicious of acitinomycetes grew five times. It was a gram-positive rod that was also partially acid-fast on modified Kinyoun

Original article PDF Hye Ryong Oh1, Dae Soo Moon2, Sook Jin Jang1,2, Xue Min Li2, Dong Min Kim3, Sang Gi Park4, Geon Park1, Young Jin Park2 Departments of 1Laboratory Medicine, 3Internal Medicine, 4Pediatrics, and 2Research Center for Resistant Cells, College of Medicine, Chosun University, Gwangju, Korea Corresponding to Dae Soo Moon, E-mail: dsmoon@chosun.ac.kr Ann Clin Microbiol 2008;11(2):123-128.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In July 2007, three neonates in the neonatal intensive care unit (NICU) of Chosun University Hospital expired due to Escherichia coli sepsis. An E. coli outbreak was suspected.  Methods: To investigate the outbreak, environmental cultures were taken from NICU. We performed repetitive extragenic palindromic (rep)-PCR to compare genotypes of the three isolates from the cases and one environmental strain of E. coli. A case-control study was done in order to identify risk factors for the infection.  Results: In July 2007, the attack rate of E. coli was 11.1%, which was higher than the basal rate. All

Original article PDF Gyun Cheol Park1, Sook Jin Jang1,2, Min Jung Lee2, Joong-Ki Kook3, Min Jung Kim3, Young Sook Kim4, Nam Woong Yang5, Hye Soo Lee6,7, Seong Ho Kang1, Geon Park1, Dae Soo Moon1 1Department of Laboratory Medicine, 2Research Center for Resistant Cells, Chosun University College of Medicine, 3Department of Oral Biochemistry, College of Dentistry, Chosun University, 4Departments of Radiology, Chosun University College of Medicine, 5Department of Microbiology, College of Medicine, Chosun University, Gwangju, 6Department of Laboratory Medicine, Chonbuk National University Medical School, 7Chonbuk National University Hospital Branch of National Culture Collection of Pathogens, Jeonju, Korea Corresponding to Sook Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2015;18(1):20-26. https://doi.org/10.5145/ACM.2015.18.1.20Copyright © Korean Society of Clinical Microbiology. Abstract Background: Recently, genotypic identification of anaerobes is emerging as an alternative to the phenotypic method. In this study, we evaluated the performance of Vitek 2, API 20A and 16s rRNA gene sequencing for the identification