Case report PDF Ji Soo Kim, M.D., Soo Yeon Park, M.D., Yeoung Chul Kil, M.T., Hee Joo Lee, M.D., and Jin Tae Suh, M.D. Department of Clinical Pathology, College of Medicine, Kyunghee University, Seoul, Korea Corresponding to Ji Soo Kim, E-mail: jakesu@yahoo.co.kr Ann Clin Microbiol 2000;3(2):147-150.Copyright © Korean Society of Clinical Microbiology. Abstract V. parahaemolyticus or V. alginolyticus infections are usually associated with consumption of raw or undercooked shellfish, contaminated food, and exposure of wounds to warm seawater. V. parahaemolyticus causes gastroenteritis (the most common syndrome), wound infections, and septicemia. V. alginolyticus occasionally causes extraintestinal infections in humans. So far, the authors have not found the report of V. parahaemolyticus and V. alginolyticus isolation from a patient. So, we report a case of concurrent isolation of V. parahaemolyticus and V. alginolyticus from a patient who had a history of intestinal diarrhea and vomiting. (Korean J Clin Microbiol 2000;3:147-150) Keywords V. parahaemolyticus, V. alginolyticus, stool culture

Case report PDF Chang Ki Kim1 , Injoo Cho1 , Youn Hee Park1, Kyoung Ho Roh1, Dongeun Yong1,3,4, Kyungwon Lee1,3,4 , June Myung Kim2,3,4, Yunsop Chong1,3 Departments of Laboratory Medicine1, Internal Medicine2; Research Institute of Bacterial Resistance3; and Brain Korea 21 Project for Medical Sciences4, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yumc.yonsei.ac.kr Ann Clin Microbiol 2006;9(1):71-75.Copyright © Korean Society of Clinical Microbiology. Abstract Haemophilus aphrophilus is a facultative anaerobic, gram-negative coccobacillus or bacillus and its growth is stimulated by 5 to 10% CO2 . Most Haemophilus species require either exogenous X or V factor or both to grow, but H. aphrophilus can grow without these factors. H. aphrophilus rarely causes invasive infections such as endocarditis, septicemia, pneumonia and peritonitis in human. Two cases of infective endocarditis by H. aphrophilus have been reported in Korea. However, there has been no report of polymicrobial endocarditis by H. aphrophilus and other bacteria. We isolated H. aphrophilus and coagulase-negative staphylococci (CNS) from

Case report PDF Kyoung-Jin Park1, Kyung Sun Park1, Soo-Han Choi2, Yae-Jean Kim2, Chang-Seok Ki1, I-Seok Kang2, Nam Yong Lee1 Departments of 1Laboratory Medicine and Genetics, 2Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Corresponding to Nam Yong Lee, E-mail: micro.lee@samsung.com Ann Clin Microbiol 2012;15(4):139-142. https://doi.org/10.5145/KJCM.2012.15.4.139Copyright © Korean Society of Clinical Microbiology. Abstract Blood culture-negative infective endocarditis (CNE) can be a diagnostic dilemma. Herein, we report a case of CNE caused by Haemophilus parainfluenzae identified only via 16S rRNA sequence analysis directly from valve tissue. A 17-year-old boy presented with high spiking fever for one month. Pansystolic murmur (Grade III) and vegetation (0.65×0.26 cm and 0.62×0.55 cm) on the anterior mitral valve leaflet via transesophageal echocardiogram suggested the diagnosis of infective endocarditis (IE). However, blood culture performed on admission was negative even after 2 weeks of incubation. Gram stain and culture of a direct tissue specimen failed to

Case report Jaewoong Lee1, Hyunjung Kim1, Hae Kyung Lee1, Yeon-Joon Park2 Department of Laboratory Medicine, 1Uijeongbu St. Mary’s Hospital, Uijeongbu, 2Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea Corresponding to Hae Kyung Lee, E-mail: hkl@catholic.ac.kr Ann Clin Microbiol 2020;23(3):155-158. https://doi.org/10.5145/ACM.2020.23.3.5Received on 14 July 2020, Revised on 6 August 2020, Accepted on 6 August 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Probiotics are used to restore and maintain the healthy intestinal microflora. Although Saccharomyces cerevisiae (SC) is considered as a non-pathogenic yeast, administration of SC as a probiotic is associated with a rare cause of fungemia in immunocompromised patients with central venous catheter insertion. We encountered a case of SC fungemia in a premature infant who presented with respiratory distress syndrome and had undergone central venous catheterization. Keywords Central venous catheter, Fungemia, Premature birth , Probiotics, Saccharomyces cerevisiae Probiotics are live

Original article PDF Wee Gyo Lee1 , Hae Kyung Lee2, Han Jin Kim2, June Key Chung2, Eun Hee Lee3, Hae Ran Moon3 Department of Laboratory Medicine1, Ajou University School of Medicine, Suwon; Ejinpediatric clinics2, Chunan; and Green Cross Reference Laboratory3, Seoul, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann Clin Microbiol 2004;7(2):119-123.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Influenza virus is a cause of annual outbreaks of acute respiratory disease and is responsible for considerable mortality and morbidity in all age groups. To achieve maximum efficacy antiinfluenza drugs must be started within 48 h of the development of influenza symptoms. Improvements in rapid diagnosis methods are needed to identify influenza infections. The aim of this study was to compare a quick rapid antigen test with viral culture assays. Methods : A total of 87 nasopharyngeal swab specimens were collected from symptomatic paediatric patients during March, 2004. The

Original article PDF Hae Kyung Lee1, Hiun Suk Chae2, Jung Oak Kang3, Mi-Kyung Lee4, Heungsup Sung5, Mi-Na Kim5, Jongwook Lee6, Miae Lee7, Ki-Nam Shim8 Departments of 1Laboratory Medicine, 2Internal Medicine, The Catholic University of Korea College of Medicine, 3Department of Laboratory Medicine, Hanyang University College of Medicine, 4Department of Laboratory Medicine, Chung-Ang University College of Medicine, 5Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, 6Hanaro Medical Foundation, Departments of 7Laboratory Medicine, 8Internal Medicine, Ewha Womans University School of Medicine, Seoul, Korea Corresponding to Miae Lee, E-mail: miae@ewha.ac.kr Ann Clin Microbiol 2008;11(2):84-89.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Clarithromycin resistance in Helicobacter pylori is a major cause of eradication therapy failure. The objective of this study was to determine the frequency and type of mutations in the 23S rRNA gene in Korea, which are associated with clarithromycin resistance.  Methods: From January 2008 to March 2008, 353 gastric biopsy specimens were collected from five university

Original article PDF Heungsup Sung1, Jung-Oak Kang2, Mi Ae Lee3, Jongwook Lee4, Hae Kyung Lee5, Mi-Kyung Lee6, Ji-Hun Lim1, Mi-Na Kim1, Helicobacter Study Group Department of Laboratory Medicine, 1University of Ulsan College of Medicine and Asan Medical Center, 2Hanyan/g University Medical College, 3Ewha Womans University College of Medicine, 4Konyang University College of Medicine, 5 The Catholic University of Korea College of Medicine, 6Chung-Ang University College of Medicine, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2009;12(1):30-36.Copyright © Korean Society of Clinical Microbiology. Abstract Background: CLSI provides a guideline only for a agar dilution method of testing clarithromycin susceptibility for Helicobacter pylori. This study was to evaluate a disk diffusion method for clarithromycin and amoxicillin. Methods: One hundred and forty clinical isolates of H. pylori isolated from May 2005 to May 2007 were tested by the CLSI agar dilution method and a disk diffusion method using 2μg (2CLR) and 15μg (15CLR) clarithromycin disks and 2μg (2AMX) and 10μg (10AMX) amoxicillin disks.

Original article PDF Seungok Lee1, Yeon-Joon Park2, Hae Kyung Lee3, Soo-Young Kim4, Ja-Young Kim5, So-Young Lee6, Jin-Kyung Yoo2 1Department of Laboratory Medicine, Incehon St. Mary’s Hospital, The Catholic University School of Medicine, Incheon, 2Department of Laboratory Medicine, Seoul St. Mary’s Hospital, The Catholic University School of Medicine, Seoul, 3Department of Laboratory Medicine, Uijeongbu St. Mary’s Hospital, The Catholic University School of Medicine, Uijeongbu, 4Department of Laboratory Medicine, St. Vincent’s Hospital, The Catholic University School of Medicine, Suwon, 5Department of Laboratory Medicine, Daejeon St. Mary’s Hospital, The Catholic University School of Medicine, Daejeon, 6Department of Laboratory Medicine, Yeouido St. Mary’s Hospital, The Catholic University School of Medicine, Seoul, Korea Corresponding to Yeon-Joon Park, E-mail: yjpk@catholic.ac.kr Ann Clin Microbiol 2013;16(1):33-38. https://doi.org/10.5145/ACM.2013.16.1.33Copyright © Korean Society of Clinical Microbiology. Abstract Background: We investigated the prevalence of various pathogens (13 enteric bacteria and 5 viruses) which cause diarrhea using multiplex PCR of stool specimens and