Original article Justin Mugiraneza1, Mijung Kwon2, Daewon Kim2, Sang-Guk Lee2, Hyukmin Lee2, Dongeun Yong2 1Department of Global Health Security, Yonsei University Graduate school of Public Health, Seoul, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2020;23(3):125-134. https://doi.org/10.5145/ACM.2020.23.3.2Received on 28 January 2020, Revised on 27 March 2020, Accepted on 27 March 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The rapid antimicrobial susceptibility testing (AST) performed on urine samples would guide the adequate choice of antibiotics for obtaining better treatment outcomes in patients. Our study aimed to evaluate the performance of the modified-EUCAST (European Committee on Antimicrobial Susceptibility Testing) rapid direct AST on urine samples. Methods: From >2,000 urine samples, a total of 128 urine samples containing bacterial counts of ≥2 × 104 CFU/mL with a uniform

Original article PDF Hyukmin Lee, M.D., Young Ah Kim, M.D., Kwang Il Park, M.D., Kyungwon Lee, M.D. and Yunsop Chong, Ph.D. Department of Clinical Pathology and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee Ann Clin Microbiol 1999;2(1):77-81.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Clostridium difficile causes antibiotic-associated diarrhea or pseudomembranous colitis by producing of toxins in patients treated with antimicrobial agents. Stool cultures for C. difficile and tests for the presence of its toxin are the most widely used methods for the diagnosis of infection. The aim of this study was to determine the usefulness of polymerase chain reaction for the detection of toxin B gene from C. difficile isolates. Methods：In this study, 85 strains of C. difficile were used, which were isolated from stool specimens of patients with suspected antibiotic-associated diarrhea or pseudomembranous colitis from 1987 to 1994 using cefoxitin-cycloserine-fructose agar. DNA of the C. difficile isolates was

Case report PDF Hyukmin Lee, M.D., Kyungja Woo, M.T., Kyungwon Lee, M.D., Yunsop Chong, Ph.D., and Joo Hang Kim, M.D.* Departments of Clinical Pathology and Internal Medicine*, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee Ann Clin Microbiol 2000;3(2):137-141.Copyright © Korean Society of Clinical Microbiology. Abstract Plesiomonas shigelloides was isolated from blood culture of a 53-year-old man with fever, who had treatment history of gastrointestinal malignancy. The patient showed neither clinical features nor hematological finding which suggest bacteremia. Identification of the isolate was delayed because of its similar characteristics with Aeromonas spp. and other gram-negative bacilli. The isolate was misinterpreted as susceptible to ampicillin by the first disk diffusion test. It may not always easy to identify P. shigelloides by conventional tests and to determine its antimicrobial susceptibility accurately, as laboratorians rarely have experience with the organism and as the organism may show unusual inhibition pattern when tested by disk diffusion method

Original article PDF Hyukmin Lee1, Eun Mi Koh2, Myung Sook Kim2, Jong Hwa Yum2, Dongeun Yong2, Wee Gyo Lee3, Kyungwon Lee2, Yunsop Chong2 1Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang; 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul; 3Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2007;10(2):114-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Enterococcus gallinarum, an organism often found in the intestine, is intrinsically resistant to low level vancomycin, but some of them are highly resistant to vancomycin due to acquisition of vanA gene. We occasionally detected both vanA carrying E. gallinarum and E. faecalis or E. faecium in the same patients, suggesting transfer of the resistance gene from the latter. In this study, the structures of Tn1546-like elements in E. gallinarum, and E. faecalis or E. faecium from the same patients were compared to determine the clinical significance of the vanA genotype E. gallinarum isolates. Methods: Six

Original article PDF Hyukmin Lee1, Chang Ki Kim2, Jongwook Lee3, Sung-Hee Lee4, Ji Young Ahn5, Seong Geun Hong6, Yeon Jun Park7, Seok Hoon Jeong8, Eui-Chong Kim9, Wee Kyo Lee10, Young Uh11, Jong Hee Shin12, Tae Yeal Choi13, Hyo-Sun Kwak14, Kyungwon Lee2 Department of Laboratory Medicine, 1Kwandong University College of Medicine, Goyang; 2Yonsei University College of Medicine, Seoul; 3Keonyang University Medical College, Daejeon; 4Cheju Hanmaeum Hospital, Jeju; 5Sooncheonhyang University College of Medicine, Gumi; 6Pochon CHA University College of Medicine, Seongnam; 7 The Catholic University of Korea College of Medicine, Seoul; 8Kosin University College of Medicine, Busan; 9Seoul National University College of Medicine, Seoul; 10Ajou University College of Medicine, Suwon; 11Yonsei University Wonju College of Medicine, Wonju; 12Chonnam National Univeristy Medical School, Gwangju; 13Hanyang University College of Medicine, Seoul; 14Food Microbiology Division, Center for Food Safety Evaluation, Korea Food and Drug Administration, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2007;10(1):59-69.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Emergence and spread of antimicrobial

Original article PDF Hyukmin Lee1, Eun-Mi Koh2, Chang Ki Kim3, Jong Hwa Yum4, Kyungwon Lee5, Yunsop Chong5 1Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, 2 Incheon Blood Center, Korean Red Cross, Incheon, 3Korean Institute of Tuberculosis, Seoul, 4Department of Clinical Laboratory Science, Dongeui University, Busan, 5Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2010;13(1):19-26. https://doi.org/10.5145/KJCM.2010.13.1.19Copyright © Korean Society of Clinical Microbiology. Abstract Background: Blood culture is essential for diagnosis of sepsis. However, usually the available blood volume is not sufficient to meet the guidelines. Thus, periodic monitoring and feedback are essential to iBackground: Recently a novel plasmid-mediated resistant mechanism that conferred high-level resistance to aminoglycoside via methylation of 16S rRNA was reported. The aims of this study were to determine the prevalence of the 16S rRNA methylase genes and

Original article PDF Hae-Sun Chung1, Hyukmin Lee2, Yangsoon Lee1, Dongeun Yong1, Seok Hoon Jeong1, Bok-Kwon Lee3, Suk-Chan Jung4, Suk-Kyung Lim4, Kyungwon Le1, Yunsop Chong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, 3Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon, 4Animal, Plant and Fisheries Quartine and Inspection Agency, Anyang, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2012;15(1):14-20. https://doi.org/10.5145/KJCM.2012.15.1.14Copyright © Korean Society of Clinical Microbiology. Abstract Background: The emergence of non-typhoidal Salmonella (NTS) with decreased susceptibilities to fluoroquinolone, ampicillin, or ceftriaxone has been reported worldwide. However, current surveillance studies of resistance among NTS in Korea are limited. Thus, the antimicrobial susceptibilities; resistance mechanisms such as extended-spectrum β-lactamase (ESBL), plasmid-mediated AmpC β-lactamase (PABL), and plasmid-mediated quinolone resistance (PMQR); and molecular epidemiologic characteristics were investigated