Original article PDF Young Uh, M.D., In Ho Jang, M.S., Kap Jun Yoon, M.D., Hyun Soo Kim*, M.D., and Hyo Youl Kim*, M.D. Departments of Clinical Pathology and Internal Medicine*, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 2001;4(1):28-32.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Vibrio species may be classified as halophilic or nonhalophilic on the basis of their requirement of NaCl for optimal growth. Recently, attention has been focused on the halophilic vibrios and Vibrio cholerae non-O1/O139 causing extraintestinal infections such as septicemia. The aim of this study is to elucidate the isolation rate and clinical manifestations of Vibrio species isolated from clinical specimens between 1996 and 2000 at Wonju Christian Hospital. Methods : Stool specimens were inoculated onto the thiosulfate-citrate-bile salt-sucrose media, blood cultures were performed by automated blood culture systems with commercial bottles, and the others were cultured according to the routine procedures. Results :

Original article PDF Kon-Hee Lee1, Wonkeun Song2, Min-Jeong Park2, Jeongwon Hyun2, Hyun Soo Kim2, Kyu Man Lee2 Departments of 1Pediatrics, 2Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Corresponding to Wonkeun Song, E-mail: swonkeun@hallym.or.kr Ann Clin Microbiol 2011;14(4):126-130. https://doi.org/10.5145/KJCM.2011.14.4.126Copyright © Korean Society of Clinical Microbiology. Abstract Background: We compared the BACTEC Peds Plus (Becton Dickinson, USA) and BacT/Alert PF (bioMerieux, France) pediatric blood culture bottles in the context of recovery and time to detection (TTD) of bacteria and fungi from pediatric patients.  Methods: Blood samples were collected for culture from pediatric patients who were hospitalized during 2010 at a university hospital. BACTEC Peds Plus and BacT/Alert PF bottles were placed in the BACTEC FX and BacT/Alert 3D blood culture system, respectively, and tested for 5 days. Bottles flagged by instruments as positive were removed from the instruments and the TTDs were recorded.  Results: A total of 5,018 sets (1

Original article PDF Wonkeun Song, Min-Jeong Park, Han-Sung Kim, Jae-Seok Kim, Hyun Soo Kim, Kyu Man Lee Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Corresponding to Wonkeun Song, E-mail: swonkeun@hallym.or.kr Ann Clin Microbiol 2011;14(1):24-29. https://doi.org/10.5145/KJCM.2011.14.1.24Copyright © Korean Society of Clinical Microbiology. Abstract Background: In 2010, the Clinical and Laboratory Standards Institute (CLSI) revised breakpoints for cephalosporins and carbapenems and indicated that extended-spectrum β-lactamase (ESBL) testing is no longer necessary for Enterobacteriaceae. We compared the results of the CLSI 2010 and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) MIC breakpoints for Enterobacteriaceae producing ESBL and/or plasmid-mediated AmpC β-lactamase (PABL).  Methods: A total of 94 well-characterized clinical isolates of Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Proteus mirabilis, Salmonella spp., Shigella spp., Citrobacter freundii, Enterobacter aerogenes, Enterobacter cloacae, and Serratia marcescens were analyzed. Of them, 57 were ESBL producers, 24 were PABL producers, and 13 were ESBL plus

Erratum PDF Kon-Hee Lee1, Wonkeun Song2, Min-Jeong Park2, Jeongwon Hyun2, Hyun Soo Kim2, Kyu Man Lee2 Departments of 1Pediatrics, 2Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Ann Clin Microbiol 2013;16(3):126-130. https://doi.org/10.5145/ACM.2013.16.3.126Copyright © Korean Society of Clinical Microbiology. There was an error in the English abstract, which has been corrected as follows. Before correction Results: A total of 5,018 sets (1 set, 1 BACTEC Peds Plus and 1 BacT/Alert PF) were evaluated. Overall, the recovery proportions for BACTEC Peds Plus and BacT/Alert PF bottles were 57% (134/195) and 69% (112/195), respectively. There was a significant difference between the 0.38% contamination rate in BacT/ Alert PF bottles and the 0.16% contamination rate in BACTEC Peds Plus bottles (P=0.035). The average TTD for all microorganisms was significantly decreased for the BACTEC Peds Plus bottles (P=0.021), but was increased for Candida parapsilosis compared to the results for the BacT/Alert PF bottles (P=0.028).

Original article PDF Wonkeun Song, Han-Sung Kim, Jae-Seok Kim, Hyun Soo Kim, Dong Hoon Shin, Saeam Shin, Min-Jeong Park Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Corresponding to Wonkeun Song, E-mail: swonkeun@hallym.or.kr Ann Clin Microbiol 2016;19(4):83-87. https://doi.org/10.5145/ACM.2016.19.4.83Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the carbapenem inactivation method (CIM) compared with the modified Hodge test (MHT) for the detection of carbapenemase-producing Gram-negative bacilli. Methods: A total of 61 isolates of carbapenemase- producing Enterobacteriaceae (CPE: 14 KPC, 7 GES- 5, 8 NDM-1, 9 VIM-2, 9 IMP-1, and 14 OXA-48-like), 34 isolates of metallo-β-lactamase (MBL)-producing Pseudomonas spp. (14 VIM-2 and 20 IMP-6), and 70 carbapenem-nonsusceptible carbapenemase-negative isolates were included. The CIM and MHT were performed for all of the isolates. To perform the CIM, a meropenem disk was incubated with a suspension of the isolate to be tested and then on Mueller-Hinton agar with the

Original article PDF Jae-Seok Kim1, Sung Mi Kim2, Hyun Soo Kim1 1Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, 2Department of Pediatrics, Busan Saint Mary Hospital, Busan, Korea Corresponding to Hyun Soo Kim, E-mail: hskim0901@empas.com Ann Clin Microbiol 2017;20(3):63-66. https://doi.org/10.5145/ACM.2017.20.3.63Copyright © Korean Society of Clinical Microbiology. Abstract Background: The introduction of rotavirus vaccines has decreased the prevalence of rotavirus infections and might have changed the distribution of rotavirus genotypes. However, neonates are not eligible for vaccination and, therefore, are at risk for rotavirus infection while in the hospital nursery or neonatal intensive care unit. Our aim was to evaluate the shift of genotypes of group A rotavirus strains among neonates cared for in two geographically distant hospitals in Korea. Methods: Analysis of rotavirus P and G genotypes was performed for 63 neonates (27 neonates in Seoul and 36 neonates in Busan) admitted to two hospitals between 2011 and 2013.

Original article PDF Bareum Gwon1,2, Eun-Jeong Yoon2, Dokyun Kim2, Hyukmin Lee2, Jong Hee Shin3, Jeong Hwan Shin4, Kyeong Seob Shin5, Young Ah Kim6, Young Uh7, Hyun Soo Kim8, Young Ree Kim9, Seok Hoon Jeong2 1Department of Clinical Pathology, Sangji University College of Science, Wonju, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 3Department of Laboratory Medicine, Chonnam National University School of Medicine, Gwangju, 4Department of Laboratory Medicine, Inje University Busan Paik Hospital, Busan, 5Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju, 6Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 7Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 8Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, School of Medicine, Jeju National University, Jeju, Korea Corresponding to Eun-Jeong Yoon, E-mail: ejyoon@yuhs.ac Ann Clin Microbiol 2019;22(1):1-8. https://doi.org/10.5145/ACM.2019.22.1.1Copyright © Korean Society of Clinical Microbiology. Abstract