Original article Heekang Choi1, Daewon Kim1,2, Mijung Kwon1, Jung-Hyun Byun3, Bonghwan Jin4, Ki-Ho Hong1, Hyukmin Lee1, Dongeun Yong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Gil Medical Center, Gachon University College of Medicine, Incheon, 3Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju, 4QuantaMatrix Inc., Seoul, Korea Corresponding to Daewon Kim, E-mail: fseraph85@gmail.com Ann Clin Microbiol 2022;25(4):109-118. https://doi.org/10.5145/ACM.2022.25.4.1Received on 1 July 2022, Revised on 7 September 2022, Accepted on 8 September 2022, Published on 20 December 2022.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Rapid antimicrobial susceptibility testing (RAST) is important for the appropriate treatment