Original article Kuenyoul Park1, Kyu-Hwa Hur2, Heungsup Sung1, Sang-Ho Choi3, Mi-Na Kim1 1Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 2Department of Laboratory Medicine, Uijeongbu Eulji Medical Center, Eulji University, Uijeongbu, 3Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea Corresponding to Heungsup Sung, E-mail: sung@amc.seoul.kr Ann Clin Microbiol 2022;25(2):41-46. https://doi.org/10.5145/ACM.2022.25.2.2Received on 11 February 2022, Revised on 1 April 2022, Accepted on 22 April 2022, Published on 20 June 2022.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The concurrent detection of human cytomegalovirus (CMV) with UL97 and UL54 mutations is crucial for prescribing adequate antiviral treatment when drug-resistant CMV infection is suspected.