Original article Hae-Gyeong Baek1, Hyun-Mi Ko2, Myung-Hee Lee1 1Department of Laboratory Medicine, Kwangju Christian Hospital, Gwangju, 2Dental Science Research Institute, Department of Oral Anatomy, School of Dentistry, Chonnam National University, Gwangju, Korea. Corresponding to Myung-Hee Lee, E-mail: purunee0820@naver.com Ann Clin Microbiol 2020;23(4):201-210. https://doi.org/10.5145/ACM.2020.23.4.5Received on 8 October 2020, Revised on 3 November 2020, Accepted on 2 December 2020, Published on 20 December 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Respiratory specimens subjected to mycobacterial detection were initially pre-treated with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) to remove the mucus and normal flora. Next, they were washed and neutralized with phosphate-buffered solution (PBS). The effectiveness of distilled water (DW) compared to PBS as a washing neutralizer during identification of mycobacteria was evaluated in this study. Methods: We analyzed the results of mycobacterial test conducted at a general hospital in Gwangju from October 2016 to September 2018. PBS and DW were used as a

PDF Original article Annals of Clinical Microbiology (Ann Clin Microbiol) 2020 December, Volume 23, Issue 4, pages 201-210. https://doi.org/10.5145/ACM.2020.23.4.5 Hae-Gyeong Baek1, Hyun-Mi Ko2, Myung-Hee Lee11Department of Laboratory Medicine, Kwangju Christian Hospital, Gwangju, 2Dental Science Research Institute, Department of Oral Anatomy, School of Dentistry, Chonnam National University, Gwangju, Korea. Abstract Background: Respiratory specimens subjected to mycobacterial detection were initially pretreated with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) to remove the mucus and normal flora. Next, they were washed and neutralized with phosphate-buffered solution (PBS). The effectiveness of distilled water (DW) compared to PBS as a washing neutralizer during identification of mycobacteria was evaluated in this study. Methods: We analyzed the results of mycobacterial test conducted at a general hospital in Gwangju from October 2016 to September 2018. PBS and DW were used as a respiratory sample washing agent for one year each. Results: The positive culture rate for the culture of mycobacteria was 12.7%

Original article PDF Jeong-Hun Kim, Jin-Tae Suh, Myung-Hee Kim, Gee-Young Kim, Sun-Ryung Her, Hee-Joo Lee, Woo-In Lee, So-Young Kang Department of Laboratory Medicine, Kyung Hee University, College of Medicine, Seoul, Korea Corresponding to Jin-Tae Suh, E-mail: Suhjt@hitel.net Ann Clin Microbiol 2004;7(2):182-185.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Tuberculosis is still one of the most seriously threatening infections in Korea, because of multidrug resistant tuberculosis. Results of antituberculosis drug susceptibility test can provide clinicians very important informations for selection of proper regimens for treatment.  Methods : In this study the results of antituberculosis drug susceptibility test of 298 cases at Kyunghee Medical Center from 2000 to 2003 were retrospectively analysed to evaluate the trend of antituberculosis drug susceptibility. The procedure of drug susceptibility test was based on the absolute concentration method using Lowenstein-Jensen solid media.  Results : The resistance rate of Mycobacterium tuberculosis to one or more drugs was increased

Case report PDF Gee-Young Kim1, Myung-Hee Kim1, Su-Yon Park1, Myung-Jae Park2, Jin-Tae Suh1, Hee-Joo Lee1 Departments of Laboratory Medicine1, and Internal Medicine2, Kyunghee University, College of Medicine, Seoul, Korea Corresponding to Hee-Joo Lee, E-mail: leehejo@khmc.or.kr Ann Clin Microbiol 2006;9(2):137-141.Copyright © Korean Society of Clinical Microbiology. Abstract Leuconostoc spp. used to be considered nonpathogens to human, but human infections have been reported including bacteremia in compromized patients. Leuconostoc spp. may be misidentified as lactobacilli, streptococci, pediococci and enterococci due to atypical biochemical tests. Leuconostoc spp. are intrinsically resistant to vancomycin. We report a case of bacteremia caused by Leuconostoc lactis identified by 16S rRNA sequencing; the isolate was not identified by commercial kits. (Korean J Clin Microbiol 2006;9(2):137-141) Keywords Leuconostoc lactis, Leuconostoc spp., 16S rRNA sequencing