Original article PDF Nam Hee Ryoo, Jung Sook Ha, Dong Suk Jeon, Jae Ryong Kim Department of Laboratory Medicine, School of Medicine, Keimyung University Corresponding to Nam Hee Ryoo, E-mail: nhryoo@dsmc.or.kr Ann Clin Microbiol 2004;7(2):178-181.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Streptococcus pneumoniae is one of the most common pathogens of communityacquired pneumonia (CAP) which needs rapid diagnosis and appropriate therapeutic approaches. We evaluated a new rapid urinary antigen test kit, NOW S. pneumoniae antigen test (Binax Inc., Maine, USA), for the detection of the S. pneumoniae antigen in the urine of patients who were suspected of CAP. Methods : A total of 115 urine samples were tested during April to July, 2004. Patients were divided into 2 groups: the first was the patients who were suspected of CAP and the second was the patients with other disorders. Urinary antigen test was performed done by immunochromatographic methods and results were read within 15

Case report PDF Nam Hee Ryoo, Won Mok Lee, Jung Sook Ha, Dong Suk Jeon, Jae Ryong Kim, Dae Gu Sohn1 and Shin Woo Kim2 Departments of Laboratory Medicine and Plastic Surgery1, Keimyung University School of Medicine; and Division of Infectious Diseases, Department of Internal Medicine2, Kyungpook University School of Medicine, Daegu, Korea Corresponding to Nam-Hee Ryoo, E-mail: nhryoo@dsmc.or.kr Ann Clin Microbiol 2005;8(2):198-201.Copyright © Korean Society of Clinical Microbiology. Abstract A 36-year-old female who initially presented with a small erythematous and swollen abscess on her left anterior tibial area was found out to have a cutaneous Mycobacterium abscessus infection. She was first treated with incision and drainage, dressing, and antibiotics. The lesion began to be aggravated and dispersed. Neither aerobic nor anaerobic bacteria was grown on blood agar plate. After a few weeks, Mycobacterium grew on Ogawa media after 6 days, and was identified as M. abscessus by PCR-restriction fragment length polymorphism. She was then treated

Case report PDF Won Mok Lee, Jung Sook Ha, Nam Hee Ryoo, Dong Seok Jeon, Jae Ryong Kim Department of Laboratory Medicine, School of Medicine, Keimyung University, Daegu, Korea Corresponding to Nam Hee Ryoo, E-mail: nhryoo@dsmc.or.kr Ann Clin Microbiol 2006;9(2):142-145.Copyright © Korean Society of Clinical Microbiology. Abstract Corynebacterium xerosis is a normal flora of the skin, mucous membrane and gastrointestinal tract. Although not usually considered to be a pathogen, it occasionally causes serious infections in immunocompromised hosts. We report a case of a shunt infection by C. xerosis developed in a 58year-old woman following the insertion of a ventriculoperitoneal shunt. The organism was also isolated from the cerebrospinal fluid and blood. The isolate was resistant to most of the antibiotics tested except for vancomycin. However, in spite of treatment with vancomycin, the patient was expired after 5 months. (Korean J Clin Microbiol 2006;9(2):142-145) Keywords Corynebacterium xerosis, Ventriculoperitoneal shunt, Fungal shunt infection

Original article PDF Chae Hoon Lee1, Hee Soon Cho1, Nam Hee Ryoo2 Department of Laboratory Medicine1, College of Medicine, Yeungnam University; and Department of Laboratory Medicine2, College of Medicine, Keimyung University, Daegu, Korea Corresponding to Chae Hoon Lee, E-mail: chlee@med.yu.ac.kr Ann Clin Microbiol 2006;9(2):125-130.Copyright © Korean Society of Clinical Microbiology. Abstract Background: For an optimum treatment of infections, appropriate antimicrobials should be selected according to the results of antibiotic susceptibility test (AST). However, the present AST does not take into account of antimicrobial concentrations in tissues, although different tissues have different distribution of antimicrobials. Thereby we intended to evaluate the usefulness of interpreting antimicrobial susceptibility depending on tissue concentrations of antimicrobials.  Methods: Gram-negative bacilli isolated from clinical specimens at Yeungnam University Hospital during the period from January to July, 2006 were evaluated retrospectively. The data on blood concentration, half life and tissue distribution of antimicrobials with variable administration route and

Case report PDF Do Hoon Kim, Jung Sook Ha, Nam Hee Ryoo, Dong Suk Jeon, Jae Ryong Kim, Young Rok Do, Ki Young Kwon Departments of Laboratory Medicine and Internal Medicine, Keimyung University School of Medicine, Daegu, Korea Corresponding to Nam Hee Ryoo, E-mail: nhryoo@dsmc.or.kr Ann Clin Microbiol 2009;12(3):138-140.Copyright © Korean Society of Clinical Microbiology. Abstract We experienced a case in which yeasts in blood sample from a patient with cervical cancer with hepatic metastasis and multiple intraperitoneal cysts interfered with platelet morphology flag in automated blood analyzer. The peripheral blood smear was performed to confirm the flag and revealed intracellular and extracellular yeasts, which were subsequently identified as Candida parapsilosis by blood culture. (Korean J Clin Microbiol 2009;12:138-140) Keywords Candida parapsilosis, Peripheral blood smear, Automated blood analyzer, Large platelet

Original article PDF Nam Hee Ryoo, Jung Sook Ha, Dong Seok Jeon, Jae Ryong Kim Department of Laboratory Medicine, School of Medicine, Keimyung University, Daegu, Korea Corresponding to Nam Hee Ryoo, E-mail: nhryoo@dsmc.or.kr Ann Clin Microbiol 2010;13(4):169-172. https://doi.org/10.5145/KJCM.2010.13.4.169Copyright © Korean Society of Clinical Microbiology. Abstract Background: Metallo-β-lactamases (MBLs) have been reported in gram negative bacilli and are becoming increasingly important clinically because the enzymes hydrolyse almost all β-lactams, including carbapenems. Thus, the present study was conducted to determine the prevalence of MBL types in imipenem-nonsusceptible Pseudomonas aeruginosa and Acinetobacter baumannii isolated from a tertiary teaching hospital.  Methods: Imipenem-nonsusceptible strains, 128 P. aeruginosa and 93 A. baumannii, were collected from clinical specimens. Identification and susceptibility tests were determined by Vitek GNI and GNS cards. MBL production was determined by modified Hodge test and imipenem-EDTA synergy test. Multiplex PCR amplification of MBL genes including blaIMP-1, blaVIM-1 and blaVIM-2 were performed.  Results: Thirty-one