Original article Seon A Jo1, Sang Hyuk Ma2, Seungjun Lee3, Sunjoo Kim3,4 1Department of Laboratory Medicine, Daewoo General Hospital, Geoje, 2Department of Pediatrics, Changwon Fatima Hospital, Changwon,3Department of Laboratory Medicine, Gyeongsang National University Changwon Hospital, Changwon, 4Gyeongsang National University, Gyeongsang Institute of Health Sciences, Jinju, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2020;23(3):117-124. https://doi.org/10.5145/ACM.2020.23.3.1Received on 3 January 2020, Revised on 8 February 2020, Accepted on 19 February 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Pharyngitis is one of the most common conditions encountered in primary health care facilities. Accurate differentiation of group A streptococcus (GAS) infection from viral infection is difficult. The STANDARD F Strep A Ag FIA (SD BIOSENSOR, Korea) is a rapid antigen detection test (RADT) that has been recently developed for diagnosing GAS pharyngitis. In this study, we evaluated the diagnostic performance of the STANDARD F Strep

Original article Justin Mugiraneza1, Mijung Kwon2, Daewon Kim2, Sang-Guk Lee2, Hyukmin Lee2, Dongeun Yong2 1Department of Global Health Security, Yonsei University Graduate school of Public Health, Seoul, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2020;23(3):125-134. https://doi.org/10.5145/ACM.2020.23.3.2Received on 28 January 2020, Revised on 27 March 2020, Accepted on 27 March 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The rapid antimicrobial susceptibility testing (AST) performed on urine samples would guide the adequate choice of antibiotics for obtaining better treatment outcomes in patients. Our study aimed to evaluate the performance of the modified-EUCAST (European Committee on Antimicrobial Susceptibility Testing) rapid direct AST on urine samples. Methods: From >2,000 urine samples, a total of 128 urine samples containing bacterial counts of ≥2 × 104 CFU/mL with a uniform

Original article PDF Da Young Kang1,2, Jun Sung Hong1, Byeol Yi Park1,2, In-Ho Jang2, Dokyun Kim1, Hyukmin Lee1, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Clinical Pathology, Sangji University College of Health Science, Wonju, Korea Corresponding to Dokyun Kim, E-mail: kyun-sky@yuhs.ac Ann Clin Microbiol 2019;22(3):61-70. https://doi.org/10.5145/ACM.2019.22.3.61Copyright © Korean Society of Clinical Microbiology. Abstract Background: Antimicrobial resistant continues to pose a threat to public health. Therefore, rapid and accurate antimicrobial susceptibility testing is very important. The objectives of this study were to evaluate the performance of the MicroScan system (Beckman Coulter, USA) with newly developed Korean Antimicrobial Susceptibility Testing Panels (KSCM panels) for antimicrobial susceptibility testing (AST) against clinical isolates in South Korea. Methods: Three KSCM panels were designed in this study. For the performance evaluation, a total of 1,325 clinical isolates including 1,027 of Gram-negative bacilli

Original article PDF Bareum Gwon1,2, Eun-Jeong Yoon2, Dokyun Kim2, Hyukmin Lee2, Jong Hee Shin3, Jeong Hwan Shin4, Kyeong Seob Shin5, Young Ah Kim6, Young Uh7, Hyun Soo Kim8, Young Ree Kim9, Seok Hoon Jeong2 1Department of Clinical Pathology, Sangji University College of Science, Wonju, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 3Department of Laboratory Medicine, Chonnam National University School of Medicine, Gwangju, 4Department of Laboratory Medicine, Inje University Busan Paik Hospital, Busan, 5Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju, 6Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 7Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 8Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, School of Medicine, Jeju National University, Jeju, Korea Corresponding to Eun-Jeong Yoon, E-mail: ejyoon@yuhs.ac Ann Clin Microbiol 2019;22(1):1-8. https://doi.org/10.5145/ACM.2019.22.1.1Copyright © Korean Society of Clinical Microbiology. Abstract

PDF Original article Annals of Clinical Microbiology (Ann Clin Microbiol) 2020 September, Volume 23, Issue 3, pages125-134. https://doi.org/10.5145/ACM.2020.23.3.2 Justin Mugiraneza1, Mijung Kwon2, Daewon Kim2, Sang-Guk Lee2, Hyukmin Lee2, Dongeun Yong21Department of Global Health Security, Yonsei University Graduate school of Public Health, Seoul, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Abstract Background: The rapid antimicrobial susceptibility testing (AST) performed on urine samples would guide the adequate choice of antibiotics for obtaining better treatment outcomes in patients. Our study aimed to evaluate the performance of the modified-EUCAST (European Committee on Antimicrobial Susceptibility Testing) rapid direct AST on urine samples. Methods: From >2,000 urine samples, a total of 128 urine samples containing bacterial counts of ≥2 × 104 CFU/mL with a uniform bacterial shape were initially included based on flow cytometry (Sysmex UF-1000i, Japan) and Gram staining, respectively. A total of 103 samples

PDF Original article Annals of Clinical Microbiology (Ann Clin Microbiol) 2020 September, Volume 23, Issue 3, pages 117-124. https://doi.org/10.5145/ACM.2020.23.3.1 Seon A Jo1, Sang Hyuk Ma2, Seungjun Lee3, Sunjoo Kim3,41Department of Laboratory Medicine, Daewoo General Hospital, Geoje, 2Department of Pediatrics, Changwon Fatima Hospital, Changwon,3Department of Laboratory Medicine, Gyeongsang National University Changwon Hospital, Changwon, 4Gyeongsang National University, Gyeongsang Institute of Health Sciences, Jinju, Korea Abstract Background: Pharyngitis is one of the most common conditions encountered in primary health care facilities. Accurate differentiation of group A streptococcus (GAS) infection from viral infection is difficult. The STANDARD F Strep A Ag FIA (SD BIOSENSOR, Korea) is a rapid antigen detection test (RADT) that has been recently developed for diagnosing GAS pharyngitis. In this study, we evaluated the diagnostic performance of the STANDARD F Strep A Ag FIA and compared the results between the RADT and conventional throat culture. Methods: Throat swab samples were obtained

Original article PDF Sollip Kim1, Heungsup Sung1, Hong Sun Jeon1, Suk Ja Park1, Sang-Hyuk Park2, Mi-Na Kim1 1Department of Laboratory Medicine, Univertisy of Ulsan College of Medicine and Asan Medical Center, 2University of Ulsan College of Medicine, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2007;10(1):44-48.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid and accurate surveillance is crucial in controlling vancomycin-resistant enterococci (VRE). Culture-based surveillance takes more than 4 days and direct polymerase chain reaction (PCR) is rapid but compromised by a low sensitivity. In this study, we evaluated the performance of an enrichment-PCR method for vanA VRE surveillance. Methods: In July 2006, 100 fecal specimens were inoculated to Enterococcosel agar (EA) and Enterococcosel broth (EB) containing 6μg/mL vancomycin. After 1 or 2 day-incubation bacterial pellets were obtained from 1 mL of blackened EB and VanA PCR were performed with DNA extract of the pellets (EB+PCR). Blackened EB were also

Original article PDF Keigo Shibayama1*, Hyukmin Lee2*, Sunjoo Kim3 1Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan, 2Department of Laboratory Medicine, Catholic Kwangdong University, Incheon, 3Department of Laboratory Medicine, Gyeongsang National University School of Medicine, Gyeongsang Health Science Institute, Jinju, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2015;18(4):111-118. https://doi.org/10.5145/ACM.2015.18.4.111Copyright © Korean Society of Clinical Microbiology. Abstract Background: A surveillance system for antibiotic resistance is well organized in both Japan and Korea; however, a comparative analysis by microorganism has not previously been conducted. Methods: We compared the latest antibiotic resistance rates of medically important pathogens, such as Staphylococcus aureus, enterococci, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, between Japan and Korea. Data were collected by JANIS (Japan Nosocomial Infections Surveillance) and KARMS (Korean Antimicrobial Resistance Monitoring System) from 2007-2012. Results: In 2012, the proportions of oxacillin-resistant S. aureus, vancomycin-resistant Enterococcus faecium (VRE), cefotaxime-resistant