Original article Changseung Liu1*, Eunjung Lee2*, Dokyun Kim1, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Clinical Pathology, Sangji University College of Science, Wonju, Korea *These authors contributed equally to this work. Corresponding to Dokyun Kim, E-mail: kyunsky@yuhs.ac Ann Clin Microbiol 2020;23(3):135-148. https://doi.org/10.5145/ACM.2020.23.3.3Received on 31 January 2020, Revised on 10 April 2020, Accepted on 10 April 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the performance of ASTA MicroIDSys (ASTA, Korea) and Bruker Biotyper (Bruker Daltonics, Germany) systems in the identification of bacterial isolates from clinical microbiology laboratory specimens during the study period. In addition, species for which the identification accuracy using MALDI-TOF MS systems was previously reported to be poor were also identified by comparing the MS results with those obtained using molecular identification. Methods: A total of

Case report PDF Hyun Yong Hwang, M.D., Seok Hoon Jeong, M.D., Hark Rim*, M.D., Mi Hyang Kim, M.D., Tae Jeon Jeong, M.T., Byeong Gil Choi, M.T. Departments of Clinical Pathology and Internal Medicine*, Kosin University College of Medicine, Pusan, Korea Corresponding to Hyun Yong Hwang Ann Clin Microbiol 1998;1(1):113-116.Copyright © Korean Society of Clinical Microbiology. Abstract A 60-year-old male with continuous ambulatory peritoneal dialysis was admitted because of abdominal discomfort and turbid dialysate. He had a history of chronic renal failure due to diabetic nephropathy. His WBC count of peripheral blood was 8,500/mm³ (neutrophil 92%), and that of dialysate was 1,400/mm³ (polymorphonuclear leukocyte 69%, lymphocyte 31%). Pure growth of Leclercia adecarboxylata was isolated from dialysate. The L. adecarboxylata isolate was susceptible to ampicillin, ampicillin/sulbactam, cephalothin, cefoperazone, cefoxitin, cefotaxime, ceftazidime, aztreonam, imipenem, gentamicin, tobramycin, amikacin, tetracycline, trimethoprim-sulfamethoxazole and ciprofloxacin. Cephalothin & amikacin were added into dialysate, and his clinical symptoms and turbidity of dialysate were

Original article PDF Byung Lip Kim*, M.D., Seok Hoon Jeong, M.D., Ja Young Koo*, M.D., Kyungwon Lee**, M.D., Yunsop Chong**, Ph.D., Tae Jeon Jeong, M.T., Hyun Yong Hwang, M.D. and Mi Hyang Kim, M.D. Department of Internal Medicine* and Clinical Pathology, College of Medicine Kosin University, Pusan, and Department of Clinical Pathology, College of Medicine Yonsei University, Seoul, Korea** Corresponding to Seok Hoon Jeong Ann Clin Microbiol 1999;2(1):28-39.Copyright © Korean Society of Clinical Microbiology. Abstract Background ： Increased isolation of extended-spectrum β-lactamase ( ESBL)-producing Enterobacteriaceae resistant to third generation cephalosporins and aztreonam has been noted recently. This study was to determine the prevalence of resistance to these drugs and ESBL in Enterobacteriaceae and to evaluate the methods for detection. Methods：During the period of October, 1997 and March, 1998, a total of 731 clinical isolates of Enterobacteriaceae were collected from patients of the Kosin Medical Center, Pusan, Korea. Antimicrobial susceptibility test by disk diffusion method and double disk synergy

Case report PDF Hyun Yong Hwang, M.D., Seok Hoon Jeong, M.D., Tae Jeon Jeong, M.T., Byeong Gil Choi, M.T., Sang Uk Lee, M.D.* and Mi Hyang Kim, M.D. Department of Clinical Pathology and Internal Medicine* Kosin University College of Medicine, Pusan, Korea Corresponding to Seok Hoon Jeong Ann Clin Microbiol 2000;3(1):79-81.Copyright © Korean Society of Clinical Microbiology. Abstract V. parahaemolyticus was isolated from blood culture of a 34-year old female patient with HCV viral hepatitis and liver cirrhosis. V. parahaemolyticus is one of the frequent causative agents of gastrointestinal infection, but rarely causes septicemia. This case is thought to be the 3rd report of V. parahaemolyticus septicemia in Korea. (Korean J Clin Microbiol 2000;3(1):79-81) Keywords Vibrio parahaemolyticus, Septicemia, Liver cirrhosis

Original article PDF Hee Bong Shin*, Seok Hoon Jeong**, Myeungsuk Kim***, Won Ho Kim****, Kyungwon Lee*, and Yunsop Chong* Departments of Clinical Pathology* and Internal Medicine****, Yonsei University College of Medicine, Seoul, Korea Departments of Clinical Pathology***, Severnce Hospital, Seoul, Korea Departments of Clinical Pathology, College of Medicine, Kosin University**, Busan, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2001;4(2):87-95.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Diarrheal disease has been one of the most common health problem in Korea with Salmonella and Shigella being the major bacterial pathogens. Prevalence of enteric bacterial pathogens may differ significantly depending on the socioeconomic status of a country. Therefore, rapid improvement of living conditions in Korea should have profound effect on the incidence of enteric infection. In some Salmonella infections, proper antimicrobial treatment is important to reduce morbidity and mortality, but rapid change of the susceptibility makes the susceptibility unpredictable. So, there is a need to

Original article PDF Jeong Man Kim, Seok Hoon Jeong,*,*****, Bit Na Kim,*, Ji Hyun Sung,**, Jong Chul Kim,***, Hyunjung Jang,**** Department of Clinical Pathology, Dong-A University College of Medicine; Departments of Clinical Pathology*, Hospital Infection Control**, and Urology***, Kosin University College of Medicine; SJ-Hightech Co., Ltd.****, Busan; Research Institute of Bacterial Resistance*****, Yonsei University College of Medicine, Seoul, Korea Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr Ann Clin Microbiol 2002;5(2):97-104.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Among Enterobacter spp. isolates from clinical specimens in Korea, the incidence of resistance to expanded-spectrum cephalosporins is becoming an ever-increasing problem. This study was designed to determine the prevalence of expanded-spectrum cephalosporins-resistant Enterobacter spp. isolates from patients in a tertiary care hospital in Busan, Korea, and to characterize the mechanism of resistance. Materials and Methods: Nonduplicated clinical isolates of Enterobacter spp. were collected during the period of 1999-2000 in Kosin Medical Center, Busan, Korea. Antimicrobial susceptibilities were tested

Original article PDF Jeong Man Kim, Seok Hoon Jeong******, Sang Hee Lee****, Ji Hye Kim**, Bit Na Kim*, and Jong Chul Kim*** Department of Clinical Pathology, Dong-A University College of Medicine; Departments of Clinical Pathology*, Internal Medicine**, and Urology***, Kosin University College of Medicine, Busan; Department of Genetic Engineering****, Youngdong University, Chungbuk; Research Institute of Bacterial Resistance*****, Yonsei University College of Medicine, Seoul, Korea Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr Ann Clin Microbiol 2002;5(1):6-14.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Among Gram-negative pathogens in Korea, the incidence of resistance to thirdgeneration cephalosporins is becoming an ever-increasing problem. This study was designed to determine the prevalence of third-generation cephalosporins-resistant Escherichia coli and Klebsiella pneumoniae isolates from patients in a tertiary care hospital in Busan, Korea, and to characterize the mechanism of resistance. Methods: A total of 710 E. coli and 237 K. pneumoniae non-duplicate isolates were collected from patients in Kosin Medical Center in 1999. Antimicrobial