Original article PDF Chang Jae Lee, M.D.*, Seung Jung Kee, M.D.*, Jong Hee Shin, M.D.*,**, Soon Pal Suh, M.D.*,** and Dong Wook Ryang, M.D.*,** Department of Clinical Pathology*, Chonnam National University Medical School; Research Institute of Medical Sciences**, Chonnam National University, Gwangju, Korea Corresponding to Soon Pal Suh, E-mail: spsuh@chonnam.ac.kr Ann Clin Microbiol 2001;4(2):115-121.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The PCR assay for the detection of M. tuberculosis has been used for 5 years in Chonnam National University Hospital. To evaluate the reliability of the PCR assay, the PCR results were compared with those of culture and clinical diagnosis. Methods : This study analyzed the results of BACTEC culture and PCR for detection of M. tuberculosis between Jan. 1996 and Dec. 1998. A total of 7,430 specimens for the PCR, 16,163 specimens for the TB BACTEC culture and 4,810 specimens (3,167 patients) submitted for both PCR and BACTEC culture were analyzed

Case report PDF Eun-Mi Koh1,3, Chang Ki Kim1,3, Myungsook Kim3, Shin-Moo Kim4, Seung-Woo Park2, Hyun Cheol Chung2, Dongeun Yong1,3, Kyungwon Lee1,3, Yunsop Chong1,3 Departments of 1Laboratory Medicine and 2Internal Medicine, and 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul; 4Department of Clinical Laboratory Science, Wonkwang Health Science College, Iksan, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2007;10(2):164-167.Copyright © Korean Society of Clinical Microbiology. Abstract Vibrio fluvialis is a haplophilic gram-negative bacterium normally found in coastal water and seafood and causes gastroenteritis. There have been a few reports on V. fluvialis gastroenteritis in Korea, but no previous report of isolation from blood. We isolated V. fluvialis from the blood of two patients undergoing cancer chemotherapy. (Korean J Clin Microbiol 2007;10:164-167) Keywords Vibrio fluvialis, Blood culture, Bacteremia

Original article PDF Da-Woon Kim, Jong Hee Shin, Seung Jung Kee, Soo-Hyun Kim, Myung Geun Shin, Soon Pal Suh, Dong Wook Ryang Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea Corresponding to Jong Hee Shin, E-mail: shinjh@chonnam.ac.kr Ann Clin Microbiol 2009;12(3):122-128.Copyright © Korean Society of Clinical Microbiology. Abstract Background: VITEK-2 yeast susceptibility test (AST- YS01; bioMerieux, Hazelwood, MO, USA) has recently been introduced as a fully automated commercial antifungal susceptibility test system that determines MIC (minimum inhibitory concentrations) endpoints spectrophotometrically, thereby eliminating subjective errors. We compared the VITEK-2 system with the CLSI (the Clinical and Laboratory Standards Institute) M27 method for susceptibility testing of Candida isolates from Korea. Methods: A total of 175 Candida bloodstream isolates were collected from two hospitals during a 18-month period. We compared the MIC results for amphotericin B, fluconazole and voriconazole obtained with the VITEK-2 system to those obtained by the CLSI M27 broth microdilution method

Case report PDF Chang-Jin Moon, Jong-Hee Shin, Eun-Sun Jeong, Seung-Jung Kee, Soo-Hyun Kim, Myung-Geun Shin, Soon-Pal Suh, Dong-Wook Ryang Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea Corresponding to Jong-Hee Shin, E-mail: shinjh@chonnam.ac.kr Ann Clin Microbiol 2009;12(2):97-101.Copyright © Korean Society of Clinical Microbiology. Abstract We describe here a case of central venous catheter (CVC)-related bacteremia caused by Microbacterium species in a 14-year-old patient, who had received chemotherapy for acute lymphoblastic leukemia. All nine blood cultures obtained from admission day 2 to day 62 yielded the same yellow-pigmented coryneform rod. Both Vitek 2 (bioMerieux, USA) and MicroScan (Dade Behring, USA) identified the isolate as Micrococcus species, and the API Coryne (bioMerieux, France) identified the isolate as Rhodococcus or Brevibacterium species. However, the 16S rRNA gene sequence showed a 99% identity with Microbacterium species. The bacteremia was recurrent or persistent over 60 days despite alternate systemic antibiotic therapy, but blood culture became negative after an addition of

Case report PDF Ja Young Lee1, Si Hyun Kim1, Haeng Soon Jeong1, Seung Hwan Oh1, Hye Ran Kim1, Young Il Yang2,4, Yang Haeng Lee3, Jeong Nyeo Lee1,4, Jeong Hwan Shin1,4 Departments of 1Laboratory Medicine, 2Pathology, 3Thoracic Surgery, 4Paik Institute for Clinical Research, Inje University College of Medicine, Busan, Korea Corresponding to Jeong Hwan Shin, E-mail: jhsmile@inje.ac.kr Ann Clin Microbiol 2009;12(2):82-86.Copyright © Korean Society of Clinical Microbiology. Abstract Aggregatibacter aphrophilus is a facultatively anaerobic gram-negative coccobacillus or bacillus that grows with no dependence on X factor and variable requirement for V factor. The organism is normal flora in the human oral cavity and upper respiratory tract and, rarely, causes invasive infections such as bacteremia, endocarditis, brain abscess, or osteomyelitis. We report a case of septic peripheral embolism in left leg from A. aphrophilus endocarditis. A 49-year-old man with known hypertension presented with acute muscle pain in the left leg. On physical examination, a regular heartbeat with a pansystolic

Original article PDF Hyeong-Kee Yun1, Soo-Hyun Kim1, Duck Cho1, Seung-Jung Kee1, Myung-Geun Shin1, Jong-Hee Shin1,2, Soon-Pal Suh1, Dong-Wook Ryang1 1Department of Laboratory Medicine, Chonnam National University Medical School and 2Clinical Trial Center, Chonnam National University Hospital, Gwangju, Korea Corresponding to Soo-Hyun Kim, E-mail: alpinboy@hanmail.net Ann Clin Microbiol 2009;12(2):67-71.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The universal sample processing (USP) method has recently been introduced as a simple technique that is applicable to smear microscopy, culture, and polymerase chain reaction (PCR) for the detection of Mycobaterium tuberculosis (MTB). The present study evaluated the utility of the USP method for detecting MTB by culture and PCR, and the results were compared with that of the N-acetyl L-cysteine (NALC)-NaOH (6%) method. Methods: All sputum specimens were digested and decontaminated by both the USP and NALC-NaOH methods, and the processed samples were inoculated for MTB culture and PCR. Culture was performed (252 samples) by using

Original article PDF Yong Jun Kwon, Seung A Byun, Min Ji Choi, Eun Jeong Won, Soo Hyun Kim, Jong Hee Shin Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea Corresponding to Jong Hee Shin, E-mail: shinjh@chonnam.ac.kr Ann Clin Microbiol 2019;22(4):81-89. https://doi.org/10.5145/ACM.2019.22.4.81Copyright © Korean Society of Clinical Microbiology. Abstract Background: Candida auris was first isolated from the ears of Japanese and Korean patients. However, the prevalence of yeast isolates from ear cultures and their antifungal susceptibility profiles in these nations remain unclear. Methods: We assessed yeast isolates recovered from ear cultures from a university hospital in Korea over a 4-year period from January 2014 to December 2017. Species identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and/or sequence analysis. Antifungal minimal inhibitory concentrations (MICs) were determined using the broth microdilution method of the Clinical and Laboratory Standards Institute. Results: Among 81 non-duplicate isolates from ear cultures,

Original article PDF Si Hyun Kim1*, Eun Hee Park2*, In Yeong Hwang2, Hyukmin Lee3, Sae Am Song4, Miae Lee5, Seungok Lee6, Soo Young Kim6, Jin Ju Kim7, Jong Hee Shin8, Seong Geun Hong9, Kyeong Seob Shin10, Sunjoo Kim11, Nam Hee Ryoo12, Woonhyoung Lee13, Sook Jin Jang14, Jeong Hwan Shin4,15 1Department of Clinical Laboratory Science, Semyung University, Jecheon, 2Department of Infectious Disease, Busan Institute of Health and Environment, Busan, 3Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Inje University College of Medicine, Busan, 5Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, 6Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, 7Department of Laboratory Medicine, Inha University College of Medicine, Incheon, 8Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, 9Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, 10Department of Laboratory Medicine, Chungbuk National University