Original article Ji-Ae Choi1, Choon-Mee Kim2, Sook-Jin Jang3, Seong-Sik Cho4, Chul-Ho Jang5, Young-Jin Ko3, Seong-Ho Kang3, Geon Park3 1Division of Antimicrobial Resistance, Center for Infectious Diseases Research, Korea National Institute of Health, KCDC, Cheongju, 2Premedical Science, 3Department of Laboratory Medicine, College of Medicine, 4Department of Laboratory Medicine, Chosun University Hospital, Gwangju, 5Department of Otolaryngology, Chonnam National University Medical School, Gwangju, Republic of Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2020;23(1):45-54. https://doi.org/10.5145/ACM.2020.23.1.45Received on 20 September 2019, Revised on 18 November 2019, Accepted on 1 December 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The emergence of multidrug-resistant Acinetobacter baumannii as a nosocomial pathogen is one of the major public health problems. The aim of this study was to evaluate the role of an efflux pump gene adeJ for the multidrug resistance of A. baumannii clinical isolates. Methods: Two groups (MDRAB and SAB)

Original article Ung-Jun Kim1, Choon-Mee Kim2, Sook-Jin Jang1, Seul-Bi Lee1, Seong-Sik Cho1, Seok-Hoon Jeong3, Young-Jin Ko1, Seong-Ho Kang1, Geon Park1, Dong-Min Kim4, Na-Ra Yoon4, Young-Joon Ahn5, Dong-hoon Lim6, Joong-Ki Kook7 1Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine, Seoul2Department of Parasitology and Tropical Medicine, Gyeongsang National University College of Medicine, Jinju;3MediCheck Research Institute, Korea Association of Health Promotion, Seoul, Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2020;23(1):11-20. https://doi.org/10.5145/ACM.2020.23.1.11Received on 29 May 2019, Revised on 11 September 2019, Accepted on 11 September 2019, Published on 20 June 2024.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Acinetobacter baumannii infection is a significant health problem worldwide due to increased drug resistance. The limited antimicrobial alternatives for the treatment of severe infections by multidrug-resistant A. baumannii (MDRAB) make the search for other therapeutic options more urgent. Linalool, the major oil compound in Coriandrum sativum, was recently

Review article PDF Sook-Jin Jang Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2003;6(2):97-102.Copyright © Korean Society of Clinical Microbiology. Abstract 병원감염은 입원환자들이 걸릴 수 있는 감염성합병증으로서 심한 공중보건 문제일뿐만아니라 질병이환과 사망의 주요한 원인으로서 매년 2백만건 이상의 병원감염례가 발생한다[1-3]. 미국전역의 338개 병원을 대상으로 병원감염률에 대해 포괄적으로 연구한 보고에 의하면 병원감염률이 5.7%에 달한다고 한다[1,2]. 병원 감염은 오늘날 중요한 문제이며 갈수록 그 중요성이 높아지고 있어서 병원마다 병원 감염을 관리할 수 있는 조직적인 체계를 구비하여 운영해야 할 필요성이 있다. 병원 감염을 조절할 수 있는 계획을 개발하는데 있어서 그 첫단계는 효율적인 감시체계를 통해 병원 감염을 효과적으로 인지하는것이다[4]. 감시활동을 광범위하게 정의하면 일관성있는 정의들을 사용하여 자료를 체계적으로 수집하고 분석할 뿐만 아니라 감염위험을 줄이기 위해 감시결과를 유포하거나 사용하는 것도 포함된다[5]. (Korean J Clin Microbiol 2003;6(2):97-102)

Review article PDF Sook-Jin Jang Department of Laboratory Medicine, Chosun University College of  Medicine, Gwangju, Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2004;7(1):9-19.Copyright © Korean Society of Clinical Microbiology. Abstract 병원 감염의 역학적 연구에 있어서 기본적인 전제는 역학적 근건에서 얻은 분리주는 클론으로 연관되어 있어 공통된 특징들을 가지는 반면 역학적으로 무관한 분리주는 동일 균종이라 하더라도 여러 가지 특징이 다르다는 것이다. 이러한 전제에 의거하여 형별 검사를 하여 양자를 구별하려는 연구들이 역학적 분석을 위해 지속적으로 수행되어져 왔다. 형별 검사는 미생물에 의해 표현되는 특징들을 검사하는 표현형적 기법과 핵산체를 검사하여 의의 유전적 요소들을 DNA에 근거한 방법으로 직접 검사하는 유전형적 기법으로 분류된다[1]. 역학적 목적으로 사용될 최소의 형별 검사는 표현형적 기법이었지만 표현형적 특징이 주관적인 기법들은 같은 균종이나 아종(subspecies)의 분리주들간의 유전적 관계를 측정하는데 필요한 분석능을 제공해 주는 경우가 드물다. 이러한 분석은 여러 아형이 제기되는 역학적 의문을 해결하는데 필수적이다. 병원 감염의 근인자와 각 개인들과의 질환 분포, 새로운 고도 독성 또는 약제-내성 균주의 출현, 상재균 또는 감염

Review article PDF Sook-Jin Jang Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Korea Corresponding to Sook Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2005;8(1):1-9.Copyright © Korean Society of Clinical Microbiology. Abstract The liberal use of antibiotics in human medicine and animal husbandry has promoted the spread of multiple antibiotic resistance. Molecular mechanisms for the acquisition of resistance genes by integron-mediated recombination was discovered recently during an intensive study of antibiotic resistance. Integrons are best known as the genetic agents responsible for the capture and spread of antibiotic resistance determinants among diverse Gram-negative clinical isolates. These DNA elements, mobilized by broad-host-range conjugative plasmids, have the ability of horizontal transfer of antibiotic resistance genes between interspecies of bacteria. The association of integrons with mobile elements promotes their vertical transmission from plasmids to the bacterial chromosome and among different replicons, contributing to the dissemination of resistance genes. Integrons have been

Original article PDF Sook-Jin Jang1,2, Jin Hee Kim1, Young Sook Kim3, Jong Hee Shin4, Geon Park1, Bidur Prasad Chaulagain1,2, Dae Soo Moon1, Young Jin Park1 Department of Laboratory Medicine1, Research Center for Resistant Cells2, and Department of Diagnostic Radiology3, Chosun University Medical School; and Department of Laboratory Medicine4, Chonnam University Medical School, Gwangju, Korea Corresponding to Young Sook Kim, E-mail: yshkim@chosun.ac.kr Ann Clin Microbiol 2006;9(1):64-70.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid detection of pathogens in blood is important in patient management, because the mortality rate associated with bloodstream infections is very high. We evaluated the efficiency of a 16S rDNA PCR assay for the detection of various pathogens in blood culture broth in a clinical laboratory. Methods: 16S rDNA PCR was performed on 221 blood culture bottles consisting of 99 culturepositive and 122 culture-negative samples. The results were compared with conventional culture methods. We also compared the

Original article PDF Hu-Lin Han1, Sook-Jin Jang1,2, Geon Park2, Jong-Hee Shin3, Sung-Heui Shin1, Young-Lae Moon4, Dae-Soo Moon2, Young-Jin Park2 1Research Center for Resistant Cells, Departments of 2Laboratory Medicine and 4Orthopaedic Surgery, College of Medicine, Chosun University, 3Department of Laboratory Medicine, College of Medicine, Chonnam National University, Gwangju, Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2007;10(2):96-101.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the usefulness of a newly developed molecular typing method of infrequent restriction site polymerase chain reaction (IRS-PCR) as an epidemiological DNA fingerprinting tool for Candida tropicalis. Methods: Thirty-two strains of C. tropicalis comprising eight sporadic strains and 24 clonal strains belonging to six clones, of which clonal type were previously confirmed by pulsed-field gel electrophoresis (PFGE), were tested by IRS-PCR to evaluate the usefulness of this technique. Twenty strains of Candida species, including C. glabrata, C. krusei, C. albicans, and C. parapsilosis, were also tested to assess the ability of IRS-PCR to discriminate among

Original article PDF Geon Park, M.D.1, Sook-Jin Jang, M.D.1,2, Ho-Jong Jeon, M.D.3, Seong-Hwan Kim, M.D.4, Mi-Ja Lee, M.D.3, Jin-Hee Kim, M.S.1, Sung-Heui Shin, M.D.2, Bidur Prasad Chaulagain, M.S.1,2, Dong-Min Kim, M.D.5, Dae-Soo Moon, M.D.1, Young-Jin Park, M.D.1 Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Sook Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2007;10(2):84-89.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In hepatocellular carcinoma (HCC), the frequency of p53 mutation and the association with hepatitis B virus (HBV) infection varies with geographic locations and risk factors. The aim of this study was to determine the frequency of codon 249 mutation of p53, p53 overexpression, and HBV DNA positivity and to observe the relationship between them in Korean HCC. Methods: We analyzed overexpression of p53 in hepatoma tissue from 17 HCC patients by immunohistochemistry (IHC), specific mutations at the third base position of

Original article PDF Young Sook Kim1, Sook-Jin Jang2 Departments of 1Radiology, and 2Laboratory Medicine, Chosun University College of Medicine, Gwangju, Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2012;15(3):79-87. https://doi.org/10.5145/KJCM.2012.15.3.79Copyright © Korean Society of Clinical Microbiology. Abstract The three components of taxonomy are classification, nomenclature and identification. Traditionally, bacterial classification and identification were performed based on the morphology and the biochemical data of the bacteria. In newer theories, or so-called natural concepts, the relationships between bacteria are based on the overall similarities of both the phenotypic and genotypic characteristics. The polyphasic taxonomy, or current taxonomy, describes the integration of all of the available genotypic, phenotypic, and phylogenetic information into a consensus type of general-purpose classification. When routine identification methods that are based on the biochemical tests fail, alternative procedures such as complete 16s rRNA gene sequence analysis are required. Although the results of 16s rRNA gene sequence analysis