A Case of Whole Genome Analysis of SARS-CoV-2 Using Oxford Nanopore MinION System
Case report Jae-Seok Kim, Sung Hee Chung, Jung-Min Kim, Hyun Soo Kim, Han-Sung Kim, Wonkeun Song Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Corresponding to Jae-Seok Kim, E-mail: jaeseok@hallym.ac.kr Ann Clin Microbiol 2021;24(4):135-140. https://doi.org/10.5145/ACM.2021.24.4.4Received on 28 June 2021, Revised on 27 September 2021, Accepted on 11 November 2021, Published on 20 December 2021.Copyright © Korean Society of Clinical Microbiology. Abstract The application of whole genome sequencing on SARS-CoV-2 viral genome is essential for our understanding of the molecular epidemiology and spread of viruses in the community. The portable whole genome sequencer MinION (Oxford Nanopore Technologies, ONT, UK) could be feasibly used in a clinical microbiology laboratory without the need of vast resources or stringent operating conditions. We used the MinION sequencer to analyze the viral genome sequence of one SARS-CoV-2 strain. In June 2020, nasopharyngeal specimen from one patient was subjected to whole-genome analysis using the




