Editorial Hae-Sun Chung Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, Korea Correspondence to Hae-Sun Chung, E-mail: sunny0521.chung@ewha.ac.kr Ann Clin Microbiol 2025;28(4):27. https://doi.org/10.5145/ACM.2025.28.4.8Received on 16 December 2025, Revised on 18 December 2025, Accepted on 18 December 2025, Published on 20 December 2025.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article which is freely available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/). The expanding role of whole-genome sequencing in clinical microbiology: A specially invited review Infectious diseases remain a leading cause of morbidity and mortality worldwide, and antimicrobial resistance (AMR) continues to erode the effectiveness of standard treatment pathways. Against this backdrop, whole-genome sequencing (WGS) has shifted from being a specialized research capability to a practical engine for clinical decision support, outbreak investigation, and pathogen surveillance. The review by the distinguished Japanese scholar Professor Takashi Takahashi [1], “Whole-genome sequencing applications for

Original article Teddy Namirimu1, Sunjoo Kim2,3,4 1Marine Biotechnology & Bioresource Research Department, Korea Institute of Ocean Science and Technology, Busan, 2Department of Laboratory Medicine, Gyeongsang National University College of Medicine, Jinju, 3Institute of Medical Science, Gyeongsang National University, Jinju, 4Department of Laboratory Medicine, Gyeongsang National University Changwon Hospital, Changwon, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2024;27(3):197-204. https://doi.org/10.5145/ACM.2024.27.3.1Received on 30 June 2024, Revised on 12 July 2024, Accepted on 30 July 2024, Published on 28 August 2024.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article which is freely available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/). Abstract Background: Dengue virus (DENV) is transmitted by mosquitoes and is becoming a global threat owing to an increase in the number of cases and mortality, especially in low- and middle-income tropical countries. Rapid and easy-to-use diagnostic tests are required to differentiate dengue fever

Original article Eun-Hwa Baek1, Se-Eun Kim1, Da-Hye Kim1, Sunjoo Kim2,3 1Infection Control Team, 2Department of Laboratory Medicine, Gyeongsang National University Changwon Hospital, Changwon, 3Institute of Health Science Gyeongsang National University, Jinju, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2020;23(4):173-179. https://doi.org/10.5145/ACM.2020.23.4.2Received on 21 February 2020, Revised on 16 April 2020, Accepted on 16 April 2020, Published on 20 December 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Multidrug- resistant organisms (MDRO) are a serious concern in healthcare-associated infections. Hand hygiene (HH) is essential to prevent the spread of MDRO in the healthcare institutes. The aim of this study was to investigate the relationship between the incidence of MDRO and hand hygiene compliance and the experimental effectiveness of alcohol-gel hand hygiene practice. Methods: From March 2016 to September 2018, we analyzed the cross-correlation between the incidence of MDRO and the HH compliance each month at Gyeongsang National University

Original article Seon A Jo1, Sang Hyuk Ma2, Seungjun Lee3, Sunjoo Kim3,4 1Department of Laboratory Medicine, Daewoo General Hospital, Geoje, 2Department of Pediatrics, Changwon Fatima Hospital, Changwon,3Department of Laboratory Medicine, Gyeongsang National University Changwon Hospital, Changwon, 4Gyeongsang National University, Gyeongsang Institute of Health Sciences, Jinju, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2020;23(3):117-124. https://doi.org/10.5145/ACM.2020.23.3.1Received on 3 January 2020, Revised on 8 February 2020, Accepted on 19 February 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Pharyngitis is one of the most common conditions encountered in primary health care facilities. Accurate differentiation of group A streptococcus (GAS) infection from viral infection is difficult. The STANDARD F Strep A Ag FIA (SD BIOSENSOR, Korea) is a rapid antigen detection test (RADT) that has been recently developed for diagnosing GAS pharyngitis. In this study, we evaluated the diagnostic performance of the STANDARD F Strep

Original article PDF Mi Ae Lee, M.D., Hyang Sook Park, M.T., Sunjoo Kim, M.D.*, and Eui Chong Kim, M.D.** Department of Clinical Pathology, College of Medicine, Ewha Womans University Mokdong Hospital, Seoul, Korea, Department of Clinical Pathology, Gyeongsang National Univeresity, Chinju, Korea*, and Department of Clinical Pathology, Seoul National University College of Medicine, Seoul, Korea** Corresponding to Mi Ae Lee, E-mail: miae@mm.ewha.ac.kr Ann Clin Microbiol 2001;4(2):96-101.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Several automated and nonautomated systems have been developed and are commercially available for the identification of gram-negative bacilli. EASY 24E+ kit was recently developed as Korean kit for identification of gram-negative bacilli. So we evaluated the accuracy and clinical utility of EASY 24E+ compared with API 20E and VITEK GNI+. Methods：The 221 clinical isolates of Enterobacteriaceae, including 17 C. freundii, 20 E. cloacae, 31 E. coli, 6 E. aerogenes, 29 K. pneumoniae, 3 K. oxytoca, 11 M. morganii, 13 P. mirabilis, 16 Salmonella  spp., 20 S. marcescens, 9 Shigella spp.,22 S. sonnei, 16 S. typhi, 8 Y. 

Original article PDF Sunjoo Kim, M.D. Department of Clinical Pathology, Gyeongsang National University, College of Medicine, Chinju, Korea Corresponding to Sunjoo Kim, E-mail: sjkim@nongae.gsnu.ac.kr Ann Clin Microbiol 2001;4(2):82-86.Copyright © Korean Society of Clinical Microbiology. Abstract Arrays are used for many purposes, most prominently to measure level of gene expression for tens of thousands of genes simultaneously. Microarrays consist of probe DNA attached to the solid surface (chips), which is hybridized with fluorescent labeled cDNA samples. Microarrays can be classified into two categories. Oligonucleotide array uses 25 bp oligonucleotide probe DNA that is synthesized in situ on the solid surface photolithographically. Avidin-phycoerythrin is used to develop fluorescence. Compared to oligonucleotide array, cDNA microarray is more flexible to customize. The size of cDNA produced by RT-PCR, ranges from 250 to 750 bp. Spotter and twocolor scanner are needed for cDNA microarray. The ratio of Cy5/Cy3 is plotted and clustered for each gene

Original article PDF Seong Geun Hong1, Sunjoo Kim2, Seok Hoon Jeong3, Chulhun L. Chang4, Sung Ran Cho5, Ji Young Ahn5, Jong Hee Shin6, Hye Soo Lee7, Won Keun Song8, Young Uh9, Jong Hwa Yum10, Dongeun Yong10, Kyungwon Lee10, Yunsop Chong10 Department of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Gyeongsang Nat. Univ. School of Med.2, Coll. of Med. Kosin Univ.3, Pusan Nat. Univ. Coll. of Med.4, Sooncheonhyang Univ. Coll. of Med.5, Chonnam Nat. Univ. Med. School6, Chonbuk Univ. Med. School7, Hanllym Univ. Coll. of Med.8, Yonsei Univ. Wonju Coll. of Med.9, Yonsei Univ. Coll. of Med.10 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(2):149-155.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Increase in extended-spectrum β-lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae have been reported in Korea. The aim of this study was to determine the nationwide prevalence of ESBL-producing E. coli and K. pneumoniae, and to investigate the types of ESBLs. Methods：A total of 2,221 E. coli and