Original article PDF Jongyoun Yi1, Jae-Kyoo Lee2, Eun-Joung Lee2 and Eui-Chong Kim1, 2 Department of Laboratory Medicine1, Seoul National University College of Medicine;Clinical Research Institute2, Seoul National University Hospital, Seoul, Korea Corresponding to Eui-Chong Kim, E-mail: euichong@snu.ac.kr Ann Clin Microbiol 2003;6(2):109-113.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Amoxicillin/clavulanate (A/C) is a combination of a broad spectrum β-lactam antibiotic amoxicillin and the potent β-lactamase inhibitor clavulanate. A/C 7:1 combination is known to be equal in its clinical efficacy and to have less gastrointestinal adverse effects compared to conventional A/C 4:1 combination. We estimated in vitro antimicrobial activities of the 7:1 combination (AMOCLA Duo) and the conventional 4:1 combination against clinical bacterial isolates known to be the major causes of acute otitis media or sinusitis. Methods : Total 183 strains isolated from clinical specimens of patients at Seoul National University Hospital were tested for minimal inhibitory concentraion (MIC). Streptococcus pneumoniae and Haemophilus influenzae were tested by

Review article PDF Wee Gyo Lee, M.D. Department of Clinical Pathology, Ajou University School of Medicine, Suwon, Korea Corresponding to Wee Gyo Lee Ann Clin Microbiol 1998;1(1):33-36.Copyright © Korean Society of Clinical Microbiology. No abstract available.

Original article PDF Wee Gyo Lee, Suck Ho Lee*, Sun Min Lee**, Ji Young Huh, Dongeun Yong***, Kyungwon Lee***, Wan Shik Shin**** and Dong Gun Lee**** Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Division of Gastroenterology*, Department of Internal Medicine, Sooncheonhyang University Cheonan Hospital, Cheonan, Department of Laboratory Medicine, Bestian Medical Center**, Yonsei University School of Medicine***, Division of Infectious Disease****, Department of Internal Medicine, Catholic University, Seoul, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann Clin Microbiol 2003;6(2):119-125.Copyright © Korean Society of Clinical Microbiology. Abstract Background : The widespread dissemination of Tn1546 has been attributed to transposition into plasmid or transferable elements. The transposition has been achieved through the activity of insertion sequences. Genetic diversity in Tn1546 includes integration of IS elements such as IS1216V, IS1251, IS1476 and IS1542. We investigated molecular typing and the distribution of insertion sequences in vanA-containing Enterococcus faecium isolated from patients in a teaching hospital. Methods

Original article PDF Ji Young Huh, Sun Min Lee*, Suck Ho Lee**, Wee Gyo Lee, Dongeun Yong***, Kyungwon Lee***, Wan Shik Shin**** and Dong Gun Lee**** Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Bestian Medical Center*, Seoul, Division of Gastroenterology**, Department of Internal Medicine, Sooncheonhyang University Cheonan Hospital, Cheonan, Department o f Laboratory Medicine***, Yonsei University School of Medicine, Division of Infectious Disease****, Department of Internal Medicine, Catholic University, Seoul, Korea Corresponding to Ji Young Huh, E-mail: heidi2030@hanmail.net Ann Clin Microbiol 2003;6(2):114-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Vancomycin-resistant enterococci (VRE) have been increasingly isolated worldwide as a nosocomial pathogen. In Korea, because avoparcin has been used as a growth promoter in animal feed, vanA-containing enterococci have been found in animals. The aim of this study is to understand the epidimiology of VRE isolated from chicken of diverse geographic areas.  Methods : Thirty eight isolates

Original article PDF Wee Gyo Lee, Young Sun Kim, Ji Young Huh Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann Clin Microbiol 2003;6(1):18-22.Copyright © Korean Society of Clinical Microbiology. Abstract Background:Nosocomial infections caused by vancomycin-resistant enterococci (VRE) are increasing problem in Korea. Until now, no nationwide study has been performed. The aim of the present study was to monitor the antimicrobial resistance of vancomycin-resistant Enterococcus faecium (VREF). Methods: Two hundred and two E. faecium isolated in 10 teaching hospital were studied. To detect VRE, the brain heart infusion agar containing 6 μg /mL vancomycin was used as the screening agar. The MIC was determined using agar dilution test. The vancomycin resistance genes (vanA, vanB & vanD)and genes (aac(6’)Ie-aph(2”) Ia & ant(6’) Ia encoding the aminoglycoside-modifying enzymes were detected by multiplex PCR using specific primers.  Results:Thirty-nine VREF were detected from 202 isolates. All had vancomycin MICs

Original article PDF Wee Gyo Lee1 , Hae Kyung Lee2, Han Jin Kim2, June Key Chung2, Eun Hee Lee3, Hae Ran Moon3 Department of Laboratory Medicine1, Ajou University School of Medicine, Suwon; Ejinpediatric clinics2, Chunan; and Green Cross Reference Laboratory3, Seoul, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann Clin Microbiol 2004;7(2):119-123.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Influenza virus is a cause of annual outbreaks of acute respiratory disease and is responsible for considerable mortality and morbidity in all age groups. To achieve maximum efficacy antiinfluenza drugs must be started within 48 h of the development of influenza symptoms. Improvements in rapid diagnosis methods are needed to identify influenza infections. The aim of this study was to compare a quick rapid antigen test with viral culture assays. Methods : A total of 87 nasopharyngeal swab specimens were collected from symptomatic paediatric patients during March, 2004. The

Original article PDF Jung Hyun Lee1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2,Gun Jo Woo3, Jongwook Lee4, Wee Gyo Lee5, Jung Oak Kang6, Ji Young Ahn7, Seong Geun Hong8, Jong Hee Shin9, Young Uh10, Yeon Jun Park11, Eui-Chong Kim12, Kyungwon Lee13, Dongeun Yong13 Departments of Pediatrics1 and Laboratory Medicine2, Kosin University College of Medicine; Division of Food Microbiology, Korea Food and Drug Administration3; Departments of Laboratory Medicine, Keonyang University College of Medicine4, Ajou University College of Medicine5, Hanyang University College of Medicine6, Sooncheonhyang University College of Medicine7, Pochon CHA University College of Medicine8, Chonnam National University Medical school9 , Yonsei University Wonju College of Medicine10, The Catholic University of Korea College of Medicine11, Seoul National University College of Medicine12, Yonsei University College of Medicine13 Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr Ann Clin Microbiol 2004;7(2):111-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background : The aims of this study were

Original article PDF Ji Hae Kang1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2*, Jongwook Lee3, Wee Gyo Lee4, Jung Oak Kang5, Ji Young Ahn6, Seong Geun Hong7, Jong Hee Shin8, Young Uh9, Yeon Jun Park10, Eui-Chong Kim11, Kyungwon Lee12, Dongeun Yong12, and Gun Jo Woo13 Departments of Pediatrics1and Laboratory Medicine2, Kosin University College of Medicine, Busan; Department of Laboratory Medicine3, Keonyang University College of Medicine, Daejeon; Department of Laboratory Medicine4, Ajou University College of Medicine, Suwon; Department of Laboratory Medicine5, Hanyang University College of Medicine, Guri; Department of Laboratory Medicine6, Sooncheonhyang University College of Medicine, Gumi; Department of Laboratory Medicine7, Pochon Cha University College of Medicine, Sungnam; Department of Laboratory Medicine8, Chonnam National University Medical School, Gwangju; Department of Laboratory Medicine9, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine10, The Catholic University of Korea College of Medicine, Seoul; Department of Laboratory Medicine11, Seoul National University

Original article PDF Wee Gyo Lee1, Il Joong Park1, Ji Young Huh2, Eui-Chong Kim2, Kyungwon Lee3, Mi-Na Kim4, Chulhun L. Chang5, Sunjoo Kim6, Young Uh7, Insoo Rheem8, Gyoung Yim Ha9, Hye Soo Lee10 Department of Laboratory Medicine1, Ajou University School of Medicine, Suwon; Department of Laboratory Medicine2, Seoul National University College of Medicine; Department of Laboratory Medicine3, Yonsei University College of Medicine; Department of Laboratory Medicine4, University of Ulsan College of Medicine, and Asan Medical Center, Seoul; Department of Laboratory Medicine5, Pusan National Uinversity School of Medicine, Busan; Department of Laboratory Medicine6, Gyeongsang University College of Medicine, Jinju; Department of Laboratory Medicine7, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine8, Dankook University College of Medicine, Chunan; Department of Laboratory Medicine9, Dongguk University College of Medicine, Gyungju; and Department of Laboratory Medicine10, Chonbuk National University College of Medicine, Chonju, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann