Case report Yeo-Jin Song1*, Bongyoung Kim1*, Young-eun Kim2, Yangsoon Lee2, Hyunjoo Pai1 1Department of Internal Medicine, 2Department of Laboratory Medicine, Hanyang University College of Medicine, Seoul, Korea *These authors contributed equally to this work. Corresponding to Hyunjoo Pai, E-mail: paihj@hanyang.ac.kr Ann Clin Microbiol 2020;23(4):211-216. https://doi.org/10.5145/ACM.2020.23.4.6Received on 19 February 2020, Revised on 10 April 2020, Accepted on 10 April 2020, Published on 20 December 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Rothia spp. are aerobic, gram-positive cocci belonging to the family Micrococcaceae, and are a part of the normal microbial flora of the human oropharynx and upper respiratory tract. We present the first case of the prosthetic valve endocarditis with cerebral hemorrhage caused by Rothia mucilaginosa in South Korea. A 65-year-old man with a prosthetic aortic valve visited the outpatient clinic with a complaint of fever. R. mucilaginosa was identified in one among four sets of blood culture bottles obtained

Case report PDF Yangsoon Lee, Eun-Mi Koh, Myungsook Kim, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, Yunsop Chong Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2010;13(3):125-127. https://doi.org/10.5145/KJCM.2010.13.3.125Copyright © Korean Society of Clinical Microbiology. Abstract Clostridium citroniae is a novel species reclassified from C. clostridioforme. Clostridium species are obligate anaerobes and spore-forming gram-positive rods. However, C. citroniae stains gram negative and does not consistently produce spores, making it difficult to identify. We isolated C. citroniae from the blood and peritoneal fluid of one patient, and from the blood of another patient, both of whom were undergoing cancer chemotherapy. (Korean J Clin Microbiol 2010;13:125-127) Keywords Clostridium citroniae, Bacteremia

Case report PDF Seri Jeong1, Ji Yeon Park1, Sang Hoon Han2, Yangsoon Lee1,3, Dongeun Yong1, Kyungwon Lee1, Yunsop Chong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, 2Department of Internal Medicine, Yonsei University College of Medicine, Seoul, 3Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2011;14(4):144-147. https://doi.org/10.5145/KJCM.2011.14.4.144Copyright © Korean Society of Clinical Microbiology. Abstract A 60-year-old man presented with a 1-day history of fever, vomiting, and diarrhea. He was diagnosed with severe septic shock on the basis of a body temperature of 38.9℃, heart rate of 92/min, respiratory rate of 25/min, WBC count of 22,970/μL, C-reactive protein (CRP) level of 136 mg/L, blood urea nitrogen (BUN) of 34.0 mg/dL, and creatinine of 2.98 mg/dL. On blood culture, Gram-positive cocci were detected in all 6 bottles. Small grayish non-hemolytic colonies were found on blood agar plates

Original article PDF Myungsook Kim, Mi Jung Kwon, Hae-Sun Chung, Yangsoon Lee, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, Yunsop Chong Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2012;15(2):60-66. https://doi.org/10.5145/KJCM.2012.15.2.60Copyright © Korean Society of Clinical Microbiology. Abstract Background: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for the identification of bacteria worldwide. To our knowledge, the evaluation of MALDI-TOF MS for the identification of bacteria in Korea has not been studied. In this paper we compared the identification results of aerobic bacteria using MALDI-TOF MS to those results using conventional biochemical methods. Methods: We evaluated the performance of a MALDI- TOF MS system (Bruker Daltonics, Leipzig, Germany) on consecutive aerobic isolates collected from January to February of 2011 which were identified using conventional methods (biochemical testing

Original article PDF Hae-Sun Chung1, Hyukmin Lee2, Yangsoon Lee1, Dongeun Yong1, Seok Hoon Jeong1, Bok-Kwon Lee3, Suk-Chan Jung4, Suk-Kyung Lim4, Kyungwon Le1, Yunsop Chong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, 3Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon, 4Animal, Plant and Fisheries Quartine and Inspection Agency, Anyang, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2012;15(1):14-20. https://doi.org/10.5145/KJCM.2012.15.1.14Copyright © Korean Society of Clinical Microbiology. Abstract Background: The emergence of non-typhoidal Salmonella (NTS) with decreased susceptibilities to fluoroquinolone, ampicillin, or ceftriaxone has been reported worldwide. However, current surveillance studies of resistance among NTS in Korea are limited. Thus, the antimicrobial susceptibilities; resistance mechanisms such as extended-spectrum β-lactamase (ESBL), plasmid-mediated AmpC β-lactamase (PABL), and plasmid-mediated quinolone resistance (PMQR); and molecular epidemiologic characteristics were investigated

Original article PDF Hyo Jin Kim1, Younghee Seo1, Wan Hee Kim1, Yangsoon Lee1, Hyukmin Lee2, Kyungwon Lee1, Yunsop Chong Department of Laboratory Medicine, 1Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Kwandong University College of Medicine, Incheon, Korea Corresponding to Hyukmin Lee, E-mail: hmlee.labmed@gmail.com Ann Clin Microbiol 2013;16(4):182-187. https://doi.org/10.5145/ACM.2013.16.4.182Copyright © Korean Society of Clinical Microbiology. Abstract Background: Antimicrobial resistance of Neisseria gonorrhoeae has become a serious problem worldwide, and ceftriaxone non-susceptible isolates have been recently reported from Japan and Europe. In this study, we evaluated the antimicrobial susceptibilities and molecular epidemiological characteristics of isolates from Korea in 2013. Methods: Sixty strains of N. gonorrhoeae were collected from Korean patients and prostitutes. Antimicrobial susceptibility was tested by the agar dilution and disk diffusion methods. N. gonorrhoeae multi-antigen sequence typing (NG-MAST) was performed in order to determine the molecular epidemiologic relatedness. Results: All of isolates were non-susceptible to penicillin

Original article PDF Youngsik Lim1, Yangsoon Lee1,2, Younghee Seo1, Jong Hwa Yum3, Dongeun Yong1, Kyungwon Lee1, Yunsop Chong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, Goyang, 3Department of Clinical Laboratory Science, Dong-eui University, Busan, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2013;16(3):120-125. https://doi.org/10.5145/ACM.2013.16.3.120Copyright © Korean Society of Clinical Microbiology. Abstract Background: Gram-negative bacilli can be stored in cystine tryptic agar (CTA) at room temperature for over 1 year, but we experienced a loss of imipenem resistance among VIM-2-producing isolates. The aims of this study were to determine the frequency of loss of IMP-1 and VIM-2 genes during storage in CTA at room temperature and to document any change in the MIC of antimicrobial agents and the location of the gene. Methods: Bacteria were isolated from clinical specimens at

Original article PDF In Joo Cho1, Jisook Yim1, Yangsoon Lee1,2, Myung Sook Kim1, Youkyung Seo1, Hae-Sun Chung1, Dongeun Yong1, Seok Hoon Jeong1, Kyungwon Lee1, Yunsop Chong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2013;16(1):45-51. https://doi.org/10.5145/ACM.2013.16.1.45Copyright © Korean Society of Clinical Microbiology. Abstract Background: Trends in the isolation of enteropathogenic bacteria may differ depending on environmental sanitation. The aims of this study were to determine trends in the isolation and antimicrobial resistance patterns of enteropathogenic bacteria over the last 10 years. Methods: We analyzed stool cultures of Salmonella spp., Shigella spp., Plesiomonas shigelloides, Yersinia spp., Vibrio spp., and Campylobacter spp. collected at Severance Hospital between 2001 and 2010. Antimicrobial susceptibility testing was performed using the disk diffusion method for nontyphoidal Salmonella (NTS)

Original article PDF Hyunmin Koo1, Younghee Seo1, Yangsoon Lee1, Hyukmin Lee2, Dongeun Yong1, Seok Hoon Jeong1, Kyungwon Lee1 Department of Laboratory Medicine, 1Yonsei University College of Medicine, Seoul, 2Catholic Kwandong University College of Medicine, Incheon, Korea Corresponding to Hyukmin Lee, E-mail: hmlee.labmed@gmail.com Ann Clin Microbiol 2014;17(4):110-114. https://doi.org/10.5145/ACM.2014.17.4.110Copyright © Korean Society of Clinical Microbiology. Abstract Background: Neisseria gonorrhoeae infection remains prevalent, and the emergence of antimicrobial resistance has made the treatment and control of gonorrhea more difficult. Therefore, it is important to compare isolation methods and transport media to overcome gonorrhea via epidemiologic understanding and to determine co-infection rates with other sexually transmitted diseases among primary-care hospitals. In this study, we determine the recovery rate of transferred specimens according to type of transport media and co-infection rate using PCR. Methods: Genital specimens were collected at three primary-care hospitals from January 2010 to November 2012 using transgrow media and commercial BD transport