Original article Young-Suk Sohn1, Jung-Hyun Byun2, Young Ah Kim3, Dong-Chun Shin4, Kyungwon Lee1 1Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju, 3Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 4Department of Environmental Health Graduate School of Public Health, Yonsei University, Seoul, Korea Corresponding to Young Ah Kim, E-mail: yakim@nhimc.or.kr Ann Clin Microbiol 2020;23(1):33-43. https://doi.org/10.5145/ACM.2020.23.1.33Received on 8 July 2019, Revised on 23 August 2019, Accepted on 23 August 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The weather has well-documented effects on infectious disease and reports suggest that summer peaks in the incidences of gram-negative bacterial infections among hospitalized patients. We evaluated how season and temperature changes affect bloodstream infection (BSI) incidences of major pathogens to understand BSI trends with

Original article PDF Jae Seok Kim, M.D., Sung Sup Park, M.D., Kyou Sup Han, M.D., Eui Chong Kim, M.D., Jin Q Kim, M.D., Myoung Hee Park, M.D. and Han Ik Cho, M.D. Department of Clinical Pathology, Seoul National University College of Medicine, Seoul, Korea Corresponding to Eui Chong Kim Ann Clin Microbiol 1999;2(1):19-27.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Phylogenetic analysis was used to determine the subtype and the probable source of transmission of human immunodeficiency virus type 1 (HIV-1). The HIV-1 is an RNA virus characterized by extensive genetic variation. To determine the extent of HIV-1 genetic variation, HIV-1 envelope V3 domain structures were analyzed and compared. In this study, we analyzed phylogenetic relationships and the subtype of Korean isolates to help the epidemiological study of HIV-1 infection. Methods：Peripheral blood mononuclear cells were collected from eight patients with AIDS. HIV-1 proviral DNA was directly amplified directly by polymerase

Original article PDF Soo Jin Park*, Sunjoo Kim Korean Red Cross Gyoungnam Blood Center, Medical Quality Management Office, Changwon*; Department of Laboratory Medicine, Gyeongsang National University School of Medicine, Jinju, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2003;6(1):7-11.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Group A streptococci (GAS) is the most common cause of bacterial pharyngitis. Recently, a high frequency of resistance to erythromycin (EM), the drug of choice for penicillinallergic patients, has been reported, especially in countries where antibiotics are overused. Resistance is classified as constitutive, inducible, or M according to the sensitivity results with EM and clindamycin (CC). These EM resistance phenotype is attributable to the ermB, ermTR, and mefA genes, respectively. Although EM resistance of GAS is a serious problem in our country, there are very few reports regarding to its mechanism.  Methods: GAS were isolated from elementary school children of Jinju in 2002. Antibiotic

Original article PDF Jin Hong Park1, Ji Young Moon1, Chulhun L. Chang2, and Yung Bu Kim1* 1Department of Microbiology and Immunology, and 2Laboratory Medicine, College of Medicine, Pusan National University, Busan, Korea Corresponding to Yung Bu Kim, E-mail: ybkim@pusan.ac.kr Ann Clin Microbiol 2005;8(2):172-178.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Most of the shigellosis outbreaks in Korea have been caused by Shigella sonnei since late 1990’s. We analyzed 36 strains of S. sonnei isolated in South Korea from 1998 to 2001 by molecular epidemiologic tools to understand genetic relationship of the outbreaks. Methods: The 36 strains of S. sonnei were tested for the presence of virulence genes (ial, ipaH, stx, set1A, set1B and sen) using polymerase chain reaction (PCR) method and for the production of Shiga-toxin using latex agglutination test. Seventeen representative strains were selected and their genetic relevance was analyzed by plasmid profile and pulsed-field gel electrophoresis(PFGE). Results: By PCR, ipaH gene was detected in all 36 strains, set1B gene in

Original article PDF Eun-Ha Koh1, Kook Young Maeng1, Sunjoo Kim1, Hyun ju Jeong2, Nam Yong Lee3 Department of Laboratory Medicine, and Institute of Health Sciences1, Gyeongsang National University School of Medicine, Jinju; Department of Laboratory Medicine2, Masan Medical Center, Masan; and Department of Laboratory Medicine3, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2006;9(1):51-57.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The erythromycin (EM) resistance rates and emm genotypes of Streptococcus pyogenes could vary by geographical location and study period. The purpose of this study, involving a large number of children, was to determine EM resistance rate and its resistance mechanism of S. pyogenes, and to compare these results with those of previous studies performed at the same area. Methods: Throat cultures were taken from 2,351 healthy children of four elementary schools from October through December, 2004 in Jinju. A total

Original article PDF Sun-Hwa Lee1, Kyoung Un Park2, Hae-Kyung Lee3, Mi Young Kim1, Jin Yong Kim1, Won Kyoung Kwon1, Lee Sun Park1 Department of Laboratory Medicine, 1Neodin Medical Institute, Seoul, 2Seoul National University Bundang Hospital, Seongnam, 3The Catholic University of Korea College of Medicine, Seoul, Korea Corresponding to Sun-Hwa Lee, E-mail: lshkim@neodin.com Ann Clin Microbiol 2009;12(4):180-185.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Group B Streptococcus (Streptococcus agalactiae, GBS) is a major cause of severe infections in neonates, including bacteremia, pneumonia, and meningitis, and is generally vertically transmitted from a colonized, pregnant woman to her infant. Penicillin is the drug of choice to treat GBS infections, because GBS strains are uniformly susceptible to penicillin. Recently, however, penicillin resistant GBS strains have been reported and the rates of erythromycin and clindamycin resistance have increased. We evaluated the perineal colonization rates and antimicrobial susceptibility of GBS strains isolated from pregnant and non-pregnant women. Methods: The antibiotic