Original article PDF Jeong-Hun Kim, Jin-Tae Suh, Myung-Hee Kim, Gee-Young Kim, Sun-Ryung Her, Hee-Joo Lee, Woo-In Lee, So-Young Kang Department of Laboratory Medicine, Kyung Hee University, College of Medicine, Seoul, Korea Corresponding to Jin-Tae Suh, E-mail: Suhjt@hitel.net Ann Clin Microbiol 2004;7(2):182-185.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Tuberculosis is still one of the most seriously threatening infections in Korea, because of multidrug resistant tuberculosis. Results of antituberculosis drug susceptibility test can provide clinicians very important informations for selection of proper regimens for treatment.  Methods : In this study the results of antituberculosis drug susceptibility test of 298 cases at Kyunghee Medical Center from 2000 to 2003 were retrospectively analysed to evaluate the trend of antituberculosis drug susceptibility. The procedure of drug susceptibility test was based on the absolute concentration method using Lowenstein-Jensen solid media.  Results : The resistance rate of Mycobacterium tuberculosis to one or more drugs was increased

Original article PDF Hye Young Yun1, Han-Sung Kim1,2, Young Kyung Lee1,2, Hee Jung Kang1,2, Jae-Seok Kim2, Wonkeun Song2, Kyu Man Lee2 Hye Young Yun1, Han-Sung Kim1,2, Young Kyung Lee1,2, Hee Jung Kang1,2, Jae-Seok Kim2, Wonkeun Song2, Kyu Man Lee2 Corresponding to Han-Sung Kim, E-mail: kimhan@hallym.ac.kr Ann Clin Microbiol 2011;14(3):97-103. https://doi.org/10.5145/KJCM.2011.14.3.97Copyright © Korean Society of Clinical Microbiology. Abstract Background: The inhibition rates for nucleic acid tests of Mycobacterium tuberculosis have been reported to range from less than 1% to more than 10%. Specimen dilution, boiling, addition of bovine serum albumin (BSA), and a silica membrane can be used to override amplification inhibitors in nucleic acid tests of M. tuberculosis. The inhibition rate for real-time PCR of M. tuberculosis (COBAS TaqMan MTB test; Roche Diagnostics, Manheim, Germany) and effective strategies to override PCR inhibitors were investigated in this study.  Methods: The inhibition rate for COBAS TaqMan MTB test was investigated in 980

Original article Kibum Jeon1, Seung Soon Lee2, Hyun Soo Kim1, Jae-Seok Kim1, Young Kyung Lee1, Wonkeun Song1, Han-Sung Kim1 1Department of Laboratory Medicine, 2Division of Infectious Diseases, Hallym University College of Medicine, Anyang, Korea Corresponding to Han-Sung Kim, E-mail: kimhs@hallym.or.kr Ann Clin Microbiol 2020;23(2):67-72. https://doi.org/10.5145/ACM.2020.23.2.3Received on 24 July 2019, Revised on 15 November 2019, Accepted on 15 November 2019, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) has emerged as an important nosocomial pathogen.The purpose of this study was to determine the effective methods for performing surveillance cultures of CRAB. Methods: Nasal and rectal swabs were obtained concurrently from hospitalized intensive care unit patients colonized with CRAB. All the samples were inoculated in CHROMagar Acinetobacter medium with CR102 (CHROMagar), MacConkey agar medium supplemented with 5 µg/mL imipenem (MCA-IPM), and triptic soy broth medium supplemented with 5 µg/ mL imipenem (TSB-IPM). CRAB

Original article Seo Young Hwang, Young Kwan Lim, Kye Won Choe, Young ho Choi, Mi-Kyung Lee Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea Corresponding to Mi-Kyung Lee, E-mail: cpworld@cau.ac.kr Ann Clin Microbiol 2024;27(3):185-196. https://doi.org/10.5145/ACM.2024.27.3.6Received on 2 February 2024, Revised on 8 September 2024, Accepted on 9 September 2024, Published on 20 September 2024.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article which is freely available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/). Abstract Background: As most Candida species cause opportunistic infections, it is helpful for patient care to determine species name of Candida spp. and their distribution in both sterile and non-sterile specimens. We aimed to investigate trends in the distribution of Candida species isolated from a hospital in Korea, along with their antifungal susceptibilities and seasonal variations. Methods: This study was conducted at the Chung-Ang University Hospital and included 8,760 different clinical specimens from

Original article Young-Suk Sohn1, Jung-Hyun Byun2, Young Ah Kim3, Dong-Chun Shin4, Kyungwon Lee1 1Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju, 3Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 4Department of Environmental Health Graduate School of Public Health, Yonsei University, Seoul, Korea Corresponding to Young Ah Kim, E-mail: yakim@nhimc.or.kr Ann Clin Microbiol 2020;23(1):33-43. https://doi.org/10.5145/ACM.2020.23.1.33Received on 8 July 2019, Revised on 23 August 2019, Accepted on 23 August 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The weather has well-documented effects on infectious disease and reports suggest that summer peaks in the incidences of gram-negative bacterial infections among hospitalized patients. We evaluated how season and temperature changes affect bloodstream infection (BSI) incidences of major pathogens to understand BSI trends with

Case report PDF Jeong Won Shin, M.D., Kyoung Ho Roh., M.D., Kyungwon Lee, M.D. and Yunsop Chong, Ph.D. Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee Ann Clin Microbiol 1999;2(2):220-224.Copyright © Korean Society of Clinical Microbiology. Abstract Group B Streptococcus (GBS, S. agalactiae) is known to be the leading cause of neonatal sepsis and meningitis and the infection has been increasingly noted in adults, particularly in those with underlying diseases. Penicillin G is the drug of choice for GBS infection. However, the MIC of penicillin for GBS is greater than that for S. pyogenes. Therefore some GBS infections may be difficult to be treated. However, in Korea, our knowledge on GBS infection is limited. We observed 7 cases of GBS bacteremia during 1993-1996 in a hospital. Of the 7 patients, 3 were less than one month of age with no known

Original article PDF Jae Lim Chung, M.D., Young Ah Kim, M.D., Hee Bong Shin, M.D., Jeong Won Shin, M.D., Kyungwon Lee, M.D., Yunsop Chong, Ph.D., Jang Hyeon Park, Ph.D.* and Won Bae Kim, Ph.D.* Department of Clinical Pathology and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Research Laboratories, Dong-A Pharm. Co., Ltd., Yongin, Kyunggi-do, Korea* Corresponding to Kyungwon Lee Ann Clin Microbiol 1999;2(2):182-193.Copyright © Korean Society of Clinical Microbiology. Abstract Background: β-lactam antibiotics are one of the most frequently used antimicrobial agents. However, with the increase of β-lactamase-producing bacteria, penicillins and 1st generation cephalosporins have become less useful. Cefatrizine and clavulanic acid combination (CTCA) was developed to restore the activity. The aim of this study was to determine the activities of CTCA against major recent clinical isolates. Methods: Aerobic and anaerobic bacteria tested were isolated from clinical specimens in Severance Hospital during 1996 to 1999. Antimicrobial