Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
Open Access, Peer Reviewed
pISSN 2288-0585 eISSN 2288-6850

Search Results for: Young Uh

Frequency of Mycobacterium tuberculosis Among M. tuberculosis Complex Strains Isolated from Clinical Specimen

Original article Hyunmi Cho1, Jong-Bae Kim2, Young Uh1 1Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 2Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2024;23(1):21-31. https://doi.org/10.5145/ACM.2020.23.1.21Received on 12 June 2019, Revised on 19 August 2019, Accepted on 19 August 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid and accurate detection of Mycobacterium tuberculosis (MTB) is of primary importance for infection control and selection of anti-tuberculosis drugs. However, most clinical laboratories report MTB complex (MTC) without reporting MTB because MTC comprising MTB, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti, Mycobacterium capraei and Mycobacterium pinnipedii have 99.9% similarity at the nucleotide level and identical 16S rRNA sequences. This study was conducted to analyze the species frequency of MTC isolates obtained from clinical specimen. Methods: Of

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Four genotypes of carbapenem-resistant Acinetobacter baumannii strains lacking OXA-23 production in Korea

Brief communication Young Ah Kim1, Seok Hoon Jeong2,3, Jong Hee Shin4, Kyeong Seob Shin5, Jeong Hwan Shin6, Young Ree Kim7, Hyun Soo Kim8, Young Uh9, Nam Hee Ryoo10 1Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 2Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, 5Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, 6Department of Laboratory Medicine and Paik Institute for Clinical Research, Inje University College of Medicine, Busan, 7Department of Laboratory Medicine, Jeju National University, College of Medicine, Jeju, 8Department of Laboratory Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, 10Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu, Korea Corresponding to Young Ah

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Isolation Rate and Biochemical Reaction of Enterobacteriaceae

Original article PDF Young Uh, M.D., Jeong Seog Son, M.D., Gyu Yel Hwang, M.D., In Ho Jang, M.D., Kap Jun Yoon, M.D., and Dong Min Seo, M.D. Departments of Clinical Pathology and Medical Information Development, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 1998;1(1):82-96.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In clinical microbiology the accurate and rapid identification of members of the family Enterobacteriaceae is essential for diagnostic and therapeutic purposes and for epidemiologic studies. Accuracy of identification system depends mainly on data base such as positive rate of biochemical reactions, relative frequency of occurrence of biotype, and isolation frequency of microorganisms. The purpose of this study was to analyze the isolation rate and biotype frequency of the family Enterobacteriaceae isolated from tertiary care hospital in Korea. Methods: Isolation frequency of the family Enterobacteriaceae isolated from clinical specimens during the period of January 1998 to June 1998

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Microplate Identification System of Enterobacteriaceae

Original article PDF Young Uh, Jeong Seog Son, Gyu Yel Hwang, In Ho Jang, Kap Jun Yoon and Dong Min Seo* Departments of Clinical Pathology and Medical Information Development*, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 1999;2(2):135-143.Copyright © Korean Society of Clinical Microbiology. Abstract Background: To access the accuracy and clinical usefulness of microplate identification (ID) system for the identification of Enterobacteriaceae, we compared microplate ID system with API 20E (bioMérieux, Marcy I’Etoile, France) Methods: Ninety-two cultures of Enterobacteriaceae and one isolate of Aeromonas species were simultaneously identified by microplate ID system and the API 20E. Twenty biochemical tests used in microplate ID system were lactose, sucrose, and H₂S in Kigler’s iron agar media; indole, sucrose, raffinose, arabinose, trehalose, adonitol, dulcitol, sorbitol, cellibiose, methy-red, phenylalanine deaminase, ornithine decarboxylase, lysine decarboxylase, arginine dihydrolase, urease, and citrate in microplate; and oxidase test. The identification was obtained by considering percent likelihood

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Erythromycin Resistance Phenotype of Streptococcus pyogenes

Original article PDF Young Uh*,** Gyu Yel Hwang*, In Ho Jang*, Jong Sun Park*, Oh-Gun Kwon*, and Kap Jun Yoon* Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju*; Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea** Corresponding to Young Uh Ann Clin Microbiol 1999;2(2):131-134.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The erythromycin-resistance rate and phenotype distribution of Streptococcus pyogenes are quite different by geographical variation and study period. The aim of the present study was to determine the evolution of resistance to erythromycin and the frequency of erythromycin resistance phenotype of S. pyogenes isolated from Wonju Christian Hospital. Methods: The minimal inhibitory concentrations (MICs) of erythromycin and clindamycin for 94 S. pyogenes isolated from clinical specimens between 1990 to 1998 were investigated. Double disk test of erythromycin (78µg) and clindamycin (25µg) were performed for 15 isolates of erythromycin-resistant S. pyogenes to evaluate the erythromycin resistance phenotype. Results: The resistance

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Antimicrobial Resistance and Serotypes in the Clinical Isolates of Group B Streptococci

Original article PDF Young Uh, M.D., In Ho Jang, M.D., Gyu Yel Hwang, M.D. and Kap Jun Yoon, M.D. Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 1999;2(1):64-70.Copyright © Korean Society of Clinical Microbiology. Abstract Background:This study is designed to provide data on the trend of resistance by year of isolation in the clinical isolates of group B streptococci(GBS) during recent eight years and to elucidate the relationship between serotypes and antimicrobial resistance patterns. Methods:The minimal inhibitory concentrations (MIC) of seven antimicrobial agents and serotypes for 150 strains of GBS isolated from clinical specimens between 1990 and 1997 were investigated. Results:The resistance rate of 150 clinical isolates of GBS were 20.0% to clindamycin, 16.0% to erythromycin, 4.0% to chloramphenicol, and 95.3% to tetracycline. None was resistant to penicillin, ceftriaxone, or vancomycin. Of the 24 isolates resistant to

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Identification and Biochemical Reactions of Enterococci by a Simplified Identification System

Original article PDF Young Uh, In Ho Jang, Gyu Yel Hwang, Kap Jun Yoon and Hyung-Hoan Lee* Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea, and Department of Biology, Kon-Kuk University, Seoul, Korea* Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 1999;2(1):58-63.Copyright © Korean Society of Clinical Microbiology. Abstract Background:The accurate and rapid identification of enterococci can provide clinician’s decision making of antimicrobial therapy because enterococci are usually multiresistant to commonly used antimicrobial agents and antimicrobial resistance patterns are different according to enterococcal species. Accuracy of identification system depends mainly on data base such as positive rate of biochemical reactions, relative frequency of occurrence of biotype, and isolation frequency of microorganisms. The purpose of this study was to analyze the isolation rate and biotype frequency of enterococci isolated from clinical specimens. Methods:We used a simplified identification system for the identification of the enterococci from clinical

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Identification, Antimicrobial Susceptibility and Epidemiology of Klebsiella species Isolated from Clinical Specimen

Original article PDF Young Uh*, Soon Deok Park, Jeong Seog Son*, Hyeun Gyeo Lee*, An Suk Jeoung*, Hyun Mi Cho*, and Kap Jun Yoon*, and Hyo Youl Kim Departments of Clinical Pathology* and Internal Medicine**, Yonsei University Wonju College of Medicine, Wonju, and Research Institute of Bacterial Resistance***, Yonsei University College of Medicine, Seoul, Korea Corresponding to Young Uh Ann Clin Microbiol 2000;3(2):99-110.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In recent years, the incidence of extended-spectrum β-lactamase (ESBL) producing Klebsiella has been steadily increased, and the newer species K. planticola and K. terrigena, formerly regarded as nonpathogen, have been reported with astonishing frequency from human infectious processes by some investigators. The aim of this study is to elucidate the isolation rate and antimicrobial susceptibility of recent clinical Klebsiella isolates. Method: For the clinical Klebsiella isolates during the period of June 1999 to May 2000, isolation frequency of Klebsiella species by specimen, departments, age, and sex were analyzed. And antimicrobial susceptibilities

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Rapid Detection of Mycobacteria using Mycobacteria Growth Indicator Tube (MGIT) and Ogawa Media

Original article PDF Oh-Gun Kwon, M.D., Hyun Mi Cho, M.T., In Ho Jang, M.S., Young Uh, M.D., and Kap Jun Yoon, M.D. Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 2000;3(2):116-120.Copyright © Korean Society of Clinical Microbiology. Abstract Background: As many as several weeks of incubation may be necessary for the recovery of mycobacteria when conventional culture media are used. Previous studies evaluating Mycobacteria Growth Indicator Tube (MGIT) as a rapid for the growth and detection of mycobacteria from clinical specimens have been reported. We compared MGIT with Ogawa media for the recovery of mycobacteria from clinical specimens. Methods: Ninety nine clinical specimens received in the laboratory of Wonju Christian Hospital from June to September 1999 were used for this study. The specimens from nonsterile body sites were digested, decontaminated, and concentrated for culture and Ziehl-Neelsen stain, and specimen

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Biochemical Tests for Differential Identification of Enterococci with VanC phenotype

Original article PDF Young Uh, M.D., In Ho Jang, M.D., Gyu Yel Hwang, M.D., Mi Kyung Lee, M.D. and Kap Jun Yoon, M.D. Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 2000;3(1):30-35.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Pigment production and acidification of ribose are most frequently used biochemical tests for the differentiation of three enterococcal species carrying vanC genes such as Enterococcus gallinarum, Enterococcus casseliflavus, and Enterococcus flavescens. However, pigment production may occasionally be negative in E. casseliflavus, and some of E. casseliflavus may be negative or delayed reaction with ribose fermentation test. So, we performed this study to find out biochemical tests capable of distinguishing the strains possessing vanC genotypes. Methods: A total of 17 enterococci composed of 14 clinical isolates with motility or pigment positive strains and three ATCC strains (E. gallinarum ATCC 49573, E. casseliflavus ATCC 25788, and E. flavescens ATCC 49997) were tested by multiplex PCR of the vanC genes (vanC-1, vanC-2, and vanC-3) and various

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