Original article Hyunmi Cho1, Jong-Bae Kim2, Young Uh1 1Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 2Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2020;23(1):21-31. https://doi.org/10.5145/ACM.2020.23.1.21Received on 12 June 2019, Revised on 19 August 2019, Accepted on 19 August 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid and accurate detection of Mycobacterium tuberculosis (MTB) is of primary importance for infection control and selection of anti-tuberculosis drugs. However, most clinical laboratories report MTB complex (MTC) without reporting MTB because MTC comprising MTB, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti, Mycobacterium capraei and Mycobacterium pinnipedii have 99.9% similarity at the nucleotide level and identical 16S rRNA sequences. This study was conducted to analyze the species frequency of MTC isolates obtained from clinical specimen. Methods: Of

Original article PDF Young Uh, M.D., Jeong Seog Son, M.D., Gyu Yel Hwang, M.D., In Ho Jang, M.D., Kap Jun Yoon, M.D., and Dong Min Seo, M.D. Departments of Clinical Pathology and Medical Information Development, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 1998;1(1):82-96.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In clinical microbiology the accurate and rapid identification of members of the family Enterobacteriaceae is essential for diagnostic and therapeutic purposes and for epidemiologic studies. Accuracy of identification system depends mainly on data base such as positive rate of biochemical reactions, relative frequency of occurrence of biotype, and isolation frequency of microorganisms. The purpose of this study was to analyze the isolation rate and biotype frequency of the family Enterobacteriaceae isolated from tertiary care hospital in Korea. Methods: Isolation frequency of the family Enterobacteriaceae isolated from clinical specimens during the period of January 1998 to June 1998

Original article PDF Young Uh, Jeong Seog Son, Gyu Yel Hwang, In Ho Jang, Kap Jun Yoon and Dong Min Seo* Departments of Clinical Pathology and Medical Information Development*, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 1999;2(2):135-143.Copyright © Korean Society of Clinical Microbiology. Abstract Background: To access the accuracy and clinical usefulness of microplate identification (ID) system for the identification of Enterobacteriaceae, we compared microplate ID system with API 20E (bioMérieux, Marcy I’Etoile, France) Methods: Ninety-two cultures of Enterobacteriaceae and one isolate of Aeromonas species were simultaneously identified by microplate ID system and the API 20E. Twenty biochemical tests used in microplate ID system were lactose, sucrose, and H₂S in Kigler’s iron agar media; indole, sucrose, raffinose, arabinose, trehalose, adonitol, dulcitol, sorbitol, cellibiose, methy-red, phenylalanine deaminase, ornithine decarboxylase, lysine decarboxylase, arginine dihydrolase, urease, and citrate in microplate; and oxidase test. The identification was obtained by considering percent likelihood

Original article PDF Young Uh*,** Gyu Yel Hwang*, In Ho Jang*, Jong Sun Park*, Oh-Gun Kwon*, and Kap Jun Yoon* Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju*; Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea** Corresponding to Young Uh Ann Clin Microbiol 1999;2(2):131-134.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The erythromycin-resistance rate and phenotype distribution of Streptococcus pyogenes are quite different by geographical variation and study period. The aim of the present study was to determine the evolution of resistance to erythromycin and the frequency of erythromycin resistance phenotype of S. pyogenes isolated from Wonju Christian Hospital. Methods: The minimal inhibitory concentrations (MICs) of erythromycin and clindamycin for 94 S. pyogenes isolated from clinical specimens between 1990 to 1998 were investigated. Double disk test of erythromycin (78µg) and clindamycin (25µg) were performed for 15 isolates of erythromycin-resistant S. pyogenes to evaluate the erythromycin resistance phenotype. Results: The resistance

Original article PDF Young Uh, M.D., In Ho Jang, M.D., Gyu Yel Hwang, M.D. and Kap Jun Yoon, M.D. Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 1999;2(1):64-70.Copyright © Korean Society of Clinical Microbiology. Abstract Background：This study is designed to provide data on the trend of resistance by year of isolation in the clinical isolates of group B streptococci(GBS) during recent eight years and to elucidate the relationship between serotypes and antimicrobial resistance patterns. Methods：The minimal inhibitory concentrations (MIC) of seven antimicrobial agents and serotypes for 150 strains of GBS isolated from clinical specimens between 1990 and 1997 were investigated. Results：The resistance rate of 150 clinical isolates of GBS were 20.0% to clindamycin, 16.0% to erythromycin, 4.0% to chloramphenicol, and 95.3% to tetracycline. None was resistant to penicillin, ceftriaxone, or vancomycin. Of the 24 isolates resistant to

Original article PDF Young Uh, In Ho Jang, Gyu Yel Hwang, Kap Jun Yoon and Hyung-Hoan Lee* Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea, and Department of Biology, Kon-Kuk University, Seoul, Korea* Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 1999;2(1):58-63.Copyright © Korean Society of Clinical Microbiology. Abstract Background：The accurate and rapid identification of enterococci can provide clinician’s decision making of antimicrobial therapy because enterococci are usually multiresistant to commonly used antimicrobial agents and antimicrobial resistance patterns are different according to enterococcal species. Accuracy of identification system depends mainly on data base such as positive rate of biochemical reactions, relative frequency of occurrence of biotype, and isolation frequency of microorganisms. The purpose of this study was to analyze the isolation rate and biotype frequency of enterococci isolated from clinical specimens. Methods：We used a simplified identification system for the identification of the enterococci from clinical