Original article PDF Kyoung-Jin Park, Kyung Sun Park, Nam Yong Lee Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Corresponding to Nam Yong Lee, E-mail: micro.lee@samsung.com Ann Clin Microbiol 2012;15(3):92-97. https://doi.org/10.5145/KJCM.2012.15.3.92Copyright © Korean Society of Clinical Microbiology. Abstract Background: The aim of the present study was to investigate the epidemiologic characteristics of extrapulmonary tuberculosis (EPTB) in Korea. In addition, the results of culture-confirmed (CC) EPTB were compared with those of clinically-diagnosed (CD) EPTB.  Methods: We retrospectively reviewed non-duplicate data of tuberculosis from the Samsung Medical Center from 1995 to 2010. A total of 6,249 and 38,726 cases of tuberculosis were CC and CD EPTB cases, respectively. The cases were categorized according to the type of specimen or by the clinically- affected sites.  Results: The proportions of EPTB among all tuberculosis cases were 12% (745/6,249) and 22% (8,608/ 38,726) of the CC and

Case report PDF Sae-Mi Lee, Young-Jin Kang, Hee Jae Huh, Chang-Seok Ki, Nam Yong Lee Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Corresponding to Hee Jae Huh, E-mail: heejae.huh@samsung.com Ann Clin Microbiol 2015;18(4):135-139. https://doi.org/10.5145/ACM.2015.18.4.135Copyright © Korean Society of Clinical Microbiology. Abstract Yokenella regensburgei, a member of the family Enterobacteriaceae, is rarely isolated in humans. Here, we report a 71-year-old man with diabetic foot infection from which Y. regensburgei was isolated. Following debridement and disarticulation of the foot, an exudate specimen was obtained, from which Gram- negative bacilli were recovered. The organism was identified as Y. regensburgei using the Vitek 2 system (bioMérieux, USA) and 16S rRNA and gyrB gene sequencing. To our knowledge, this is the first case of Y. regensburgei isolation in Korea. (Ann Clin Microbiol 2015;18:135-139) Keywords Diabetic foot, DNA sequencing, Yokenella regensburgei

Original article Ji-Ae Choi1, Choon-Mee Kim2, Sook-Jin Jang3, Seong-Sik Cho4, Chul-Ho Jang5, Young-Jin Ko3, Seong-Ho Kang3, Geon Park3 1Division of Antimicrobial Resistance, Center for Infectious Diseases Research, Korea National Institute of Health, KCDC, Cheongju, 2Premedical Science, 3Department of Laboratory Medicine, College of Medicine, 4Department of Laboratory Medicine, Chosun University Hospital, Gwangju, 5Department of Otolaryngology, Chonnam National University Medical School, Gwangju, Republic of Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2020;23(1):45-54. https://doi.org/10.5145/ACM.2020.23.1.45Received on 20 September 2019, Revised on 18 November 2019, Accepted on 1 December 2019, Published on 20 March 2019.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The emergence of multidrug-resistant Acinetobacter baumannii as a nosocomial pathogen is one of the major public health problems. The aim of this study was to evaluate the role of an efflux pump gene adeJ for the multidrug resistance of A. baumannii clinical isolates. Methods: Two groups (MDRAB and SAB)

Original article Ung-Jun Kim1, Choon-Mee Kim2, Sook-Jin Jang1, Seul-Bi Lee1, Seong-Sik Cho1, Seok-Hoon Jeong3, Young-Jin Ko1, Seong-Ho Kang1, Geon Park1, Dong-Min Kim4, Na-Ra Yoon4, Young-Joon Ahn5, Dong-hoon Lim6, Joong-Ki Kook7 1Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine, Seoul2Department of Parasitology and Tropical Medicine, Gyeongsang National University College of Medicine, Jinju;3MediCheck Research Institute, Korea Association of Health Promotion, Seoul, Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2020;23(1):11-20. https://doi.org/10.5145/ACM.2020.23.1.11Received on 29 May 2019, Revised on 11 September 2019, Accepted on 11 September 2019, Published on 20 June 2024.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Acinetobacter baumannii infection is a significant health problem worldwide due to increased drug resistance. The limited antimicrobial alternatives for the treatment of severe infections by multidrug-resistant A. baumannii (MDRAB) make the search for other therapeutic options more urgent. Linalool, the major oil compound in Coriandrum sativum, was recently

Original article PDF Hu-Lin Han1, Sook-Jin Jang1,2, Geon Park2, Jong-Hee Shin3, Sung-Heui Shin1, Young-Lae Moon4, Dae-Soo Moon2, Young-Jin Park2 1Research Center for Resistant Cells, Departments of 2Laboratory Medicine and 4Orthopaedic Surgery, College of Medicine, Chosun University, 3Department of Laboratory Medicine, College of Medicine, Chonnam National University, Gwangju, Korea Corresponding to Sook-Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2007;10(2):96-101.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the usefulness of a newly developed molecular typing method of infrequent restriction site polymerase chain reaction (IRS-PCR) as an epidemiological DNA fingerprinting tool for Candida tropicalis. Methods: Thirty-two strains of C. tropicalis comprising eight sporadic strains and 24 clonal strains belonging to six clones, of which clonal type were previously confirmed by pulsed-field gel electrophoresis (PFGE), were tested by IRS-PCR to evaluate the usefulness of this technique. Twenty strains of Candida species, including C. glabrata, C. krusei, C. albicans, and C. parapsilosis, were also tested to assess the ability of IRS-PCR to discriminate among

Original article PDF Geon Park, M.D.1, Sook-Jin Jang, M.D.1,2, Ho-Jong Jeon, M.D.3, Seong-Hwan Kim, M.D.4, Mi-Ja Lee, M.D.3, Jin-Hee Kim, M.S.1, Sung-Heui Shin, M.D.2, Bidur Prasad Chaulagain, M.S.1,2, Dong-Min Kim, M.D.5, Dae-Soo Moon, M.D.1, Young-Jin Park, M.D.1 Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Sook Jin Jang, E-mail: sjbjang@chosun.ac.kr Ann Clin Microbiol 2007;10(2):84-89.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In hepatocellular carcinoma (HCC), the frequency of p53 mutation and the association with hepatitis B virus (HBV) infection varies with geographic locations and risk factors. The aim of this study was to determine the frequency of codon 249 mutation of p53, p53 overexpression, and HBV DNA positivity and to observe the relationship between them in Korean HCC. Methods: We analyzed overexpression of p53 in hepatoma tissue from 17 HCC patients by immunohistochemistry (IHC), specific mutations at the third base position of

Original article PDF Ji-Hyun Cho1,2,3, Hye-Soo Lee4, Key-Earn Lee1,2, Do-Sim Park1,2,3, Young-Jin Lee1,2,3, Hyung-Bae Moon2,3,5, Chang-Soo Choi2,3,6, Eun-Young Cho2,3,6, Haak-Cheoul Kim2,3,6 Departments of 1Laboratory Medicine, 5Pathology and 6Internal Medicine, 2Institute of Wonkwang Medical Science, 3Wonkwang Clinical Medicine Research Center, Wonkwang University College of Medicine, Iksan; 4Department of Laboratory Medicine, Chonbuk National University College of Medicine, Jeonju, Korea Corresponding to Haak-Cheoul Kim, E-mail: kimpb@wonkwang.ac.kr Ann Clin Microbiol 2007;10(1):25-31.Copyright © Korean Society of Clinical Microbiology. Abstract Background: As oral antiviral treatment for chronic hepatitis B increases, quantitation of viral load has become an essential test for HBV management, and assays using real-time PCR principles have been introduced recently. Methods: We analysed the analytical performance (precision, linear range, and sensitivity) of RealArt HBV LC PCR Reagents (Artus GmbH, Hamburg, Germany), its correlation with COBAS AMPLICOR HBV MONITOR Test (Roche Diagnostics, Mannheim, Germany), and distribution of viral load in the patients’ sera according to antiviral treatment and presence of HBeAg.

Case report PDF Koung-Sun Lee1, Do-Sim Park1,3, Ji-Hyun Cho1,3, Hak-Yeol Kim2,3, Young-Jin Lee1,3 Departments of 1Labroatory Medicine, 2Internal Medicine, 3Wonkwang Medical Science, School of Medicine, Wonkwang University, Iksan, Korea Corresponding to Young-Jin Lee, E-mail: jin20@wku.ac.kr Ann Clin Microbiol 2010;13(4):178-181. https://doi.org/10.5145/KJCM.2010.13.4.178Copyright © Korean Society of Clinical Microbiology. Abstract Balantidium coli is the only largest ciliated protozoon known to infect human and nonhuman primates. Balantidiasis is a zoonotic disease and is acquired by humans via fecal-oral contact between pigs and humans. The clinical manifestation includes mainly gastrointestinal symptoms; diarrhea and abdominal pain, but in rare cases extraintestinal spread to lungs has been reported. A few reports of B. coli were found in vaginal secretion, skin, gastric juice, and omentum, but there have been no previous isolated cases in the respiratory tract in Korea. We reported that the first case of pneumonia caused by B. coli in Korea in an immunocompetent 40-year-old woman

Case report PDF Kyoung-Jin Park1, Kyung Sun Park1, Soo-Han Choi2, Yae-Jean Kim2, Chang-Seok Ki1, I-Seok Kang2, Nam Yong Lee1 Departments of 1Laboratory Medicine and Genetics, 2Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Corresponding to Nam Yong Lee, E-mail: micro.lee@samsung.com Ann Clin Microbiol 2012;15(4):139-142. https://doi.org/10.5145/KJCM.2012.15.4.139Copyright © Korean Society of Clinical Microbiology. Abstract Blood culture-negative infective endocarditis (CNE) can be a diagnostic dilemma. Herein, we report a case of CNE caused by Haemophilus parainfluenzae identified only via 16S rRNA sequence analysis directly from valve tissue. A 17-year-old boy presented with high spiking fever for one month. Pansystolic murmur (Grade III) and vegetation (0.65×0.26 cm and 0.62×0.55 cm) on the anterior mitral valve leaflet via transesophageal echocardiogram suggested the diagnosis of infective endocarditis (IE). However, blood culture performed on admission was negative even after 2 weeks of incubation. Gram stain and culture of a direct tissue specimen failed to