Original article Seunghoo Lee, Kyu-Hwa Hur, Yunsil Chung, Heungsup Sung, Mi-Na Kim Departments of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2021;24(2):45-53. https://doi.org/10.5145/ACM.2021.24.2.3Received on 13 April 2021, Revised on 12 May 2021, Accepted on 13 May 2021, Published on 20 June 2021.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid detection of carbapenemase-producing Enterobacterales (CPE) is desirable to guide antimicrobial therapy and infection control. The NG-Test Carba5 (Carba5; NG Biotech, France) rapid multiplex lateral flow immunoassay and BD MAX Check-Points CPO Assay (CPO; BD Diagnostic Systems, USA) fully automated real-time PCR assay were evaluated for the detection of KPC, NDM, VIM, IMP, and OXA-48-like group in a culture colony compared to genotyping using conventional PCR.  Methods: Among the clinical isolates of carbapenem-resistant Enterobacterales (CRE) collected from 2013 to 2019, up to 20 isolates for