Original article Young Ah Kim1, Ea Wha Kang2, Young Hee Seo3, Kyungwon Lee3,4 1Department of Laboratory Medicine, 2Department of Internal Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 3Research Institute of Bacterial Resistance and 4Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea Corresponding to Young Ah Kim, E-mail: yakim@nhimc.or.kr Ann Clin Microbiol 2021;24(1):11-20. https://doi.org/10.5145/ACM.2021.24.1.2Received on 4 October 2020, Revised on 30 October 2020, Accepted on 3 November 2020, Published on 20 March 2021.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The purpose of this study was to analyze the clinical and microbiological characteristics of recurrent urinary tract infection (UTI) caused by Escherichia coli— the most common etiological agent. Methods: Cases of recurrent and single episodes of UTI caused by E. coli were evaluated retrospectively for a period of 6 months (January-June 2019) to analyze the clinical and molecular characteristics of this disease. Results: Healthcare-associated UTI, E. coli

Original article PDF Jung Oak Kang, M.D., Jeong Don Chae, M.D., Jeong In Eom, M.D., Dongsoo Han, M.D., Pil Whan Park*, M.D., Ile Kyu Park, M.D. and Tae Yeal Choi, M.D.** Department of Clinical Pathology and Internal Medicine, Hanyang University College of Medicine, Seoul, Korea, Department of Clinical Pathology, Gachon Medical College* Corresponding to Jung Oak Kang, E-mail: jokang@email.hanyang.ac.kr Ann Clin Microbiol 2000;3(1):43-47.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The most reliable and accurate diagnostic method of Clostridium difficile-associated disease (CAD) is considered the detection of toxin B in stool using the cell culture cytotoxicity assay. But cytotoxicity assay needs cell culture facilities and labor intensive. We evaluated an automated enzyme-linked fluorescent immunoassay for the detection of Clostridium difficile (C. difficile) toxin A in stool specimens. Methods: Two hundred sixty-seven stool specimens were cultured anaerobically on cycloserine-cefoxitin-fructose-egg yolk (CCFA) media and tested for toxin A with the VIDAS C. difficile toxin A II (CDA

Original article PDF Sung Ha Kang, Young Ree Kim Department of Laboratory Medicine, College of Medicine, Cheju National University, Jeju, Korea Corresponding to Young Ree Kim, E-mail: namu8790@empal.com Ann Clin Microbiol 2008;11(1):11-17.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The referral hospital is somewhat isolated from the mainland due to its island status; thus, microorganisms isolated from blood cultures might have a distinct pattern in their frequency and antibiogram. We attempted to uncover these characteristics. Methods: The isolates from blood cultures at the Cheju University Hospital during 2003∼2007 were analysed. After inoculation in aerobic and anaerobic bottles, blood specimens were cultured using BacT/ Alert system, and the isolates were identifieded and antimicrobial susceptibilities were tested using Vitek II system. Results: The overall positive rate of blood cultures was 9.6% and contamination rate was 3.6%. The most commonly isolated pathogens were Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae. Gram positive rod, gram negative

Original article PDF Heungsup Sung1, Jung-Oak Kang2, Mi Ae Lee3, Jongwook Lee4, Hae Kyung Lee5, Mi-Kyung Lee6, Ji-Hun Lim1, Mi-Na Kim1, Helicobacter Study Group Department of Laboratory Medicine, 1University of Ulsan College of Medicine and Asan Medical Center, 2Hanyan/g University Medical College, 3Ewha Womans University College of Medicine, 4Konyang University College of Medicine, 5 The Catholic University of Korea College of Medicine, 6Chung-Ang University College of Medicine, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2009;12(1):30-36.Copyright © Korean Society of Clinical Microbiology. Abstract Background: CLSI provides a guideline only for a agar dilution method of testing clarithromycin susceptibility for Helicobacter pylori. This study was to evaluate a disk diffusion method for clarithromycin and amoxicillin. Methods: One hundred and forty clinical isolates of H. pylori isolated from May 2005 to May 2007 were tested by the CLSI agar dilution method and a disk diffusion method using 2μg (2CLR) and 15μg (15CLR) clarithromycin disks and 2μg (2AMX) and 10μg (10AMX) amoxicillin disks.