Original article PDF Hyukmin Lee1, Eun-Mi Koh2, Chang Ki Kim3, Jong Hwa Yum4, Kyungwon Lee5, Yunsop Chong5 1Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, 2 Incheon Blood Center, Korean Red Cross, Incheon, 3Korean Institute of Tuberculosis, Seoul, 4Department of Clinical Laboratory Science, Dongeui University, Busan, 5Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2010;13(1):19-26. https://doi.org/10.5145/KJCM.2010.13.1.19Copyright © Korean Society of Clinical Microbiology. Abstract Background: Blood culture is essential for diagnosis of sepsis. However, usually the available blood volume is not sufficient to meet the guidelines. Thus, periodic monitoring and feedback are essential to iBackground: Recently a novel plasmid-mediated resistant mechanism that conferred high-level resistance to aminoglycoside via methylation of 16S rRNA was reported. The aims of this study were to determine the prevalence of the 16S rRNA methylase genes and

Original article PDF Hae-Sun Chung1, Hyukmin Lee2, Yangsoon Lee1, Dongeun Yong1, Seok Hoon Jeong1, Bok-Kwon Lee3, Suk-Chan Jung4, Suk-Kyung Lim4, Kyungwon Le1, Yunsop Chong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, 3Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health, Cheongwon, 4Animal, Plant and Fisheries Quartine and Inspection Agency, Anyang, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2012;15(1):14-20. https://doi.org/10.5145/KJCM.2012.15.1.14Copyright © Korean Society of Clinical Microbiology. Abstract Background: The emergence of non-typhoidal Salmonella (NTS) with decreased susceptibilities to fluoroquinolone, ampicillin, or ceftriaxone has been reported worldwide. However, current surveillance studies of resistance among NTS in Korea are limited. Thus, the antimicrobial susceptibilities; resistance mechanisms such as extended-spectrum β-lactamase (ESBL), plasmid-mediated AmpC β-lactamase (PABL), and plasmid-mediated quinolone resistance (PMQR); and molecular epidemiologic characteristics were investigated

Original article PDF Hyo Jin Kim1, Younghee Seo1, Wan Hee Kim1, Yangsoon Lee1, Hyukmin Lee2, Kyungwon Lee1, Yunsop Chong Department of Laboratory Medicine, 1Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Kwandong University College of Medicine, Incheon, Korea Corresponding to Hyukmin Lee, E-mail: hmlee.labmed@gmail.com Ann Clin Microbiol 2013;16(4):182-187. https://doi.org/10.5145/ACM.2013.16.4.182Copyright © Korean Society of Clinical Microbiology. Abstract Background: Antimicrobial resistance of Neisseria gonorrhoeae has become a serious problem worldwide, and ceftriaxone non-susceptible isolates have been recently reported from Japan and Europe. In this study, we evaluated the antimicrobial susceptibilities and molecular epidemiological characteristics of isolates from Korea in 2013. Methods: Sixty strains of N. gonorrhoeae were collected from Korean patients and prostitutes. Antimicrobial susceptibility was tested by the agar dilution and disk diffusion methods. N. gonorrhoeae multi-antigen sequence typing (NG-MAST) was performed in order to determine the molecular epidemiologic relatedness. Results: All of isolates were non-susceptible to penicillin

Original article PDF Hyunmin Koo1, Younghee Seo1, Yangsoon Lee1, Hyukmin Lee2, Dongeun Yong1, Seok Hoon Jeong1, Kyungwon Lee1 Department of Laboratory Medicine, 1Yonsei University College of Medicine, Seoul, 2Catholic Kwandong University College of Medicine, Incheon, Korea Corresponding to Hyukmin Lee, E-mail: hmlee.labmed@gmail.com Ann Clin Microbiol 2014;17(4):110-114. https://doi.org/10.5145/ACM.2014.17.4.110Copyright © Korean Society of Clinical Microbiology. Abstract Background: Neisseria gonorrhoeae infection remains prevalent, and the emergence of antimicrobial resistance has made the treatment and control of gonorrhea more difficult. Therefore, it is important to compare isolation methods and transport media to overcome gonorrhea via epidemiologic understanding and to determine co-infection rates with other sexually transmitted diseases among primary-care hospitals. In this study, we determine the recovery rate of transferred specimens according to type of transport media and co-infection rate using PCR. Methods: Genital specimens were collected at three primary-care hospitals from January 2010 to November 2012 using transgrow media and commercial BD transport

Original article PDF Keigo Shibayama1*, Hyukmin Lee2*, Sunjoo Kim3 1Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan, 2Department of Laboratory Medicine, Catholic Kwangdong University, Incheon, 3Department of Laboratory Medicine, Gyeongsang National University School of Medicine, Gyeongsang Health Science Institute, Jinju, Korea Corresponding to Sunjoo Kim, E-mail: sjkim8239@hanmail.net Ann Clin Microbiol 2015;18(4):111-118. https://doi.org/10.5145/ACM.2015.18.4.111Copyright © Korean Society of Clinical Microbiology. Abstract Background: A surveillance system for antibiotic resistance is well organized in both Japan and Korea; however, a comparative analysis by microorganism has not previously been conducted. Methods: We compared the latest antibiotic resistance rates of medically important pathogens, such as Staphylococcus aureus, enterococci, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, between Japan and Korea. Data were collected by JANIS (Japan Nosocomial Infections Surveillance) and KARMS (Korean Antimicrobial Resistance Monitoring System) from 2007-2012. Results: In 2012, the proportions of oxacillin-resistant S. aureus, vancomycin-resistant Enterococcus faecium (VRE), cefotaxime-resistant

Original article PDF Da Young Kang1,2, Jun Sung Hong1, Byeol Yi Park1,2, In-Ho Jang2, Dokyun Kim1, Hyukmin Lee1, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Clinical Pathology, Sangji University College of Health Science, Wonju, Korea Corresponding to Dokyun Kim, E-mail: kyun-sky@yuhs.ac Ann Clin Microbiol 2019;22(3):61-70. https://doi.org/10.5145/ACM.2019.22.3.61Copyright © Korean Society of Clinical Microbiology. Abstract Background: Antimicrobial resistant continues to pose a threat to public health. Therefore, rapid and accurate antimicrobial susceptibility testing is very important. The objectives of this study were to evaluate the performance of the MicroScan system (Beckman Coulter, USA) with newly developed Korean Antimicrobial Susceptibility Testing Panels (KSCM panels) for antimicrobial susceptibility testing (AST) against clinical isolates in South Korea. Methods: Three KSCM panels were designed in this study. For the performance evaluation, a total of 1,325 clinical isolates including 1,027 of Gram-negative bacilli

Original article PDF Si Hyun Kim1*, Eun Hee Park2*, In Yeong Hwang2, Hyukmin Lee3, Sae Am Song4, Miae Lee5, Seungok Lee6, Soo Young Kim6, Jin Ju Kim7, Jong Hee Shin8, Seong Geun Hong9, Kyeong Seob Shin10, Sunjoo Kim11, Nam Hee Ryoo12, Woonhyoung Lee13, Sook Jin Jang14, Jeong Hwan Shin4,15 1Department of Clinical Laboratory Science, Semyung University, Jecheon, 2Department of Infectious Disease, Busan Institute of Health and Environment, Busan, 3Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Inje University College of Medicine, Busan, 5Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, 6Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, 7Department of Laboratory Medicine, Inha University College of Medicine, Incheon, 8Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, 9Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, 10Department of Laboratory Medicine, Chungbuk National University

Original article PDF Bareum Gwon1,2, Eun-Jeong Yoon2, Dokyun Kim2, Hyukmin Lee2, Jong Hee Shin3, Jeong Hwan Shin4, Kyeong Seob Shin5, Young Ah Kim6, Young Uh7, Hyun Soo Kim8, Young Ree Kim9, Seok Hoon Jeong2 1Department of Clinical Pathology, Sangji University College of Science, Wonju, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 3Department of Laboratory Medicine, Chonnam National University School of Medicine, Gwangju, 4Department of Laboratory Medicine, Inje University Busan Paik Hospital, Busan, 5Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju, 6Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 7Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 8Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, School of Medicine, Jeju National University, Jeju, Korea Corresponding to Eun-Jeong Yoon, E-mail: ejyoon@yuhs.ac Ann Clin Microbiol 2019;22(1):1-8. https://doi.org/10.5145/ACM.2019.22.1.1Copyright © Korean Society of Clinical Microbiology. Abstract

PDF Original article Annals of Clinical Microbiology (Ann Clin Microbiol) 2020 September, Volume 23, Issue 3, pages125-134. https://doi.org/10.5145/ACM.2020.23.3.2 Justin Mugiraneza1, Mijung Kwon2, Daewon Kim2, Sang-Guk Lee2, Hyukmin Lee2, Dongeun Yong21Department of Global Health Security, Yonsei University Graduate school of Public Health, Seoul, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Abstract Background: The rapid antimicrobial susceptibility testing (AST) performed on urine samples would guide the adequate choice of antibiotics for obtaining better treatment outcomes in patients. Our study aimed to evaluate the performance of the modified-EUCAST (European Committee on Antimicrobial Susceptibility Testing) rapid direct AST on urine samples. Methods: From >2,000 urine samples, a total of 128 urine samples containing bacterial counts of ≥2 × 104 CFU/mL with a uniform bacterial shape were initially included based on flow cytometry (Sysmex UF-1000i, Japan) and Gram staining, respectively. A total of 103 samples

Original article Vandarith Nov1, Le Phuong Nguyen2,3, Kennedy Mensah Osei*1, Hyukmin Lee3, Dongeun Yong3, Kyungwon Lee3 1Department of Global Health Security, Yonsei University Graduate school of Public Health, Seoul, 2Brain Korea 21 plus Program for Medical Science, Yonsei University College of Medicine, Seoul, 3Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2021;24(4):115-126. https://doi.org/10.5145/ACM.2021.24.4.2Received on 19 February 2021, Revised on 24 August 2021, Accepted on 9 September 2021, Published on 20 December 2021.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Environmental sanitation plays a significant role on the prevalence of enteropathogenic bacteria. This study aimed to determine the trends in the prevalence and antimicrobial resistance profiles of enteropathogenic bacteria from 2011 to 2019. Methods: A retrospective analysis was performed using data from stool cultures of Salmonella spp., Shigella spp., Plesiomonas shigelloides, Yersinia