Erratum PDF Kon-Hee Lee1, Wonkeun Song2, Min-Jeong Park2, Jeongwon Hyun2, Hyun Soo Kim2, Kyu Man Lee2 Departments of 1Pediatrics, 2Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Ann Clin Microbiol 2013;16(3):126-130. https://doi.org/10.5145/ACM.2013.16.3.126Copyright © Korean Society of Clinical Microbiology. There was an error in the English abstract, which has been corrected as follows. Before correction Results: A total of 5,018 sets (1 set, 1 BACTEC Peds Plus and 1 BacT/Alert PF) were evaluated. Overall, the recovery proportions for BACTEC Peds Plus and BacT/Alert PF bottles were 57% (134/195) and 69% (112/195), respectively. There was a significant difference between the 0.38% contamination rate in BacT/ Alert PF bottles and the 0.16% contamination rate in BACTEC Peds Plus bottles (P=0.035). The average TTD for all microorganisms was significantly decreased for the BACTEC Peds Plus bottles (P=0.021), but was increased for Candida parapsilosis compared to the results for the BacT/Alert PF bottles (P=0.028).

Original article PDF Wonkeun Song, Han-Sung Kim, Jae-Seok Kim, Hyun Soo Kim, Dong Hoon Shin, Saeam Shin, Min-Jeong Park Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Corresponding to Wonkeun Song, E-mail: swonkeun@hallym.or.kr Ann Clin Microbiol 2016;19(4):83-87. https://doi.org/10.5145/ACM.2016.19.4.83Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the carbapenem inactivation method (CIM) compared with the modified Hodge test (MHT) for the detection of carbapenemase-producing Gram-negative bacilli. Methods: A total of 61 isolates of carbapenemase- producing Enterobacteriaceae (CPE: 14 KPC, 7 GES- 5, 8 NDM-1, 9 VIM-2, 9 IMP-1, and 14 OXA-48-like), 34 isolates of metallo-β-lactamase (MBL)-producing Pseudomonas spp. (14 VIM-2 and 20 IMP-6), and 70 carbapenem-nonsusceptible carbapenemase-negative isolates were included. The CIM and MHT were performed for all of the isolates. To perform the CIM, a meropenem disk was incubated with a suspension of the isolate to be tested and then on Mueller-Hinton agar with the

Original article PDF Jae-Seok Kim1, Sung Mi Kim2, Hyun Soo Kim1 1Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, 2Department of Pediatrics, Busan Saint Mary Hospital, Busan, Korea Corresponding to Hyun Soo Kim, E-mail: hskim0901@empas.com Ann Clin Microbiol 2017;20(3):63-66. https://doi.org/10.5145/ACM.2017.20.3.63Copyright © Korean Society of Clinical Microbiology. Abstract Background: The introduction of rotavirus vaccines has decreased the prevalence of rotavirus infections and might have changed the distribution of rotavirus genotypes. However, neonates are not eligible for vaccination and, therefore, are at risk for rotavirus infection while in the hospital nursery or neonatal intensive care unit. Our aim was to evaluate the shift of genotypes of group A rotavirus strains among neonates cared for in two geographically distant hospitals in Korea. Methods: Analysis of rotavirus P and G genotypes was performed for 63 neonates (27 neonates in Seoul and 36 neonates in Busan) admitted to two hospitals between 2011 and 2013.

Original article PDF Bareum Gwon1,2, Eun-Jeong Yoon2, Dokyun Kim2, Hyukmin Lee2, Jong Hee Shin3, Jeong Hwan Shin4, Kyeong Seob Shin5, Young Ah Kim6, Young Uh7, Hyun Soo Kim8, Young Ree Kim9, Seok Hoon Jeong2 1Department of Clinical Pathology, Sangji University College of Science, Wonju, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 3Department of Laboratory Medicine, Chonnam National University School of Medicine, Gwangju, 4Department of Laboratory Medicine, Inje University Busan Paik Hospital, Busan, 5Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju, 6Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 7Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 8Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, School of Medicine, Jeju National University, Jeju, Korea Corresponding to Eun-Jeong Yoon, E-mail: ejyoon@yuhs.ac Ann Clin Microbiol 2019;22(1):1-8. https://doi.org/10.5145/ACM.2019.22.1.1Copyright © Korean Society of Clinical Microbiology. Abstract

Original article Kibum Jeon1, Seung Soon Lee2, Hyun Soo Kim1, Jae-Seok Kim1, Young Kyung Lee1, Wonkeun Song1, Han-Sung Kim1 1Department of Laboratory Medicine, 2Division of Infectious Diseases, Hallym University College of Medicine, Anyang, Korea Corresponding to Han-Sung Kim, E-mail: kimhs@hallym.or.kr Ann Clin Microbiol 2020;23(2):67-72. https://doi.org/10.5145/ACM.2020.23.2.3Received on 24 July 2019, Revised on 15 November 2019, Accepted on 15 November 2019, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) has emerged as an important nosocomial pathogen.The purpose of this study was to determine the effective methods for performing surveillance cultures of CRAB. Methods: Nasal and rectal swabs were obtained concurrently from hospitalized intensive care unit patients colonized with CRAB. All the samples were inoculated in CHROMagar Acinetobacter medium with CR102 (CHROMagar), MacConkey agar medium supplemented with 5 µg/mL imipenem (MCA-IPM), and triptic soy broth medium supplemented with 5 µg/ mL imipenem (TSB-IPM). CRAB

Review article Eun-Jung Cho1, Eun Jin Lee1, Younggil Cha2,3 Nuri Lee1, Ki Ho Hong4, Hee Jin Huh5, Young Joo Cha6, Hyun Soo Kim1 1Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, 2Molecular Diagnostic Division, Bioneer Corp., Seoul, 3College of Pharmacy, Kangwon National University, Chuncheon, 4Department of Laboratory Medicine, Seoul Medical Center, Seoul, 5Department of Laboratory Medicine, Dongguk University College of Medicine, Goyang, 6Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea Corresponding to Hyun Soo Kim, E-mail: hskim0901@empas.com Ann Clin Microbiol 2020;23(2):57-66. https://doi.org/10.5145/ACM.2020.23.2.2Received on 1 November 2019, Revised on 24 December 2019, Accepted on 24 December 2019, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Molecular diagnostic techniques are used for the diagnosis and monitoring of viral infections. The performance of the in vitro diagnostic assays is important for an accurate and prompt diagnosis. Positive clinical samples or reference materials (RMs) are essential

Case report Jae-Seok Kim, Sung Hee Chung, Jung-Min Kim, Hyun Soo Kim, Han-Sung Kim, Wonkeun Song Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Corresponding to Jae-Seok Kim, E-mail:  jaeseok@hallym.ac.kr Ann Clin Microbiol 2021;24(4):135-140. https://doi.org/10.5145/ACM.2021.24.4.4Received on 28 June 2021, Revised on 27 September 2021, Accepted on 11 November 2021, Published on 20 December 2021.Copyright © Korean Society of Clinical Microbiology. Abstract The application of whole genome sequencing on SARS-CoV-2 viral genome is essential for our understanding of the molecular epidemiology and spread of viruses in the community. The portable whole genome sequencer MinION (Oxford Nanopore Technologies, ONT, UK) could be feasibly used in a clinical microbiology laboratory without the need of vast resources or stringent operating conditions. We used the MinION sequencer to analyze the viral genome sequence of one SARS-CoV-2 strain. In June 2020, nasopharyngeal specimen from one patient was subjected to whole-genome analysis using the

Original article Goeun Kang, Hyun Soo Kim, Han-Sung Kim, Wonkeun Song, Jae-Seok Kim Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea Corresponding to Jae-Seok Kim, E-mail:  jaeseok@hallym.ac.kr Ann Clin Microbiol 2021;24(3):105-110. https://doi.org/10.5145/ACM.2021.24.3.5Received on 9 July 2021, Revised on 19 Jult 2021, Accepted on 19 July 2021, Published on 20 September 2021.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Staphylococcus aureus is a common colonizer of the nasal vestibule and is found in approximately 20%–30% of healthy adults, while Staphylococcus epidermidis appears to be the most frequent colonizer in all regions of the upper respiratory tract. Esp, a serine protease of S. epidermidis, was reported to inhibit S. aureus colonization. This study was performed to examine the nasal colonization of S. aureus and S. epidermidis and the presence of esp determinants. Methods: Nasal swab specimens from 54 patients were cultured on blood agar plates (BAP) and selective

Mini review Hyun Soo Kim Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, Korea Corresponding to Hyun Soo Kim, E-mail: hskim@empas.com Ann Clin Microbiol 2021;24(3):67-73. https://doi.org/10.5145/ACM.2021.24.3.1Received on 5 April 2021, Revised on 15 May 2021, Accepted on 9 June 2021, Published on 20 September 2021.Copyright © Korean Society of Clinical Microbiology. Abstract SARS-CoV-2 antibody assay is a test that checks whether an antibody against the SARSCoV-2 virus has been formed in the blood after SARS-CoV-2 infection or vaccination. SARSCoV-2 antibody is detected 1–2 weeks after infection, and antibodies are produced in more than 90% of infected patients. The duration for the formation of antibodies differs by individual and by type of antibody. In the case of IgG, it is at least several months or longer, and the relationship between antibodies and immunity is being studied. As test methods, enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay (CIA), immunochromatographic assay, and