Original article PDF Seong Geun Hong1, Dongeun Yong2, Kyungwon Lee2, Eui-Chong Kim3, Wee Kyo Lee4, Seok Hoon Jeong5, Won Keun Song6, Yeon Jun Park7, Mi-Na Kim8, Young Uh9, Jong Hee Shin10, Jongwook Lee11, Ji Young Ahn12, Sun Wha Lee13, Jae Seok Kim5, Hee Bong Shin1 Departments of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Yonsei Univ. Coll. of Med.2 Seoul Nat. Univ. Coll. of Med.3, Ajou Univ. of Med.4, Coll. of Med. Kosin Univ.5, Hanllym Univ. Coll. of Med.6 The Catholic Univ. of Kor.7, Univ. of Ulsan Med. Coll. and Asan Med. Center8, Yonsei Univ. Wonju Coll. of Med.9 Chonnam Nat. Univ. Med. School10, Keonyang Univ. Med. Coll.11 Sooncheonhyang Univ. Coll. of Med.12; Neodin Med. Res. Center13 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(1):29-36.Copyright © Korean Society of Clinical Microbiology. Abstract Background: A rapid increase of antimicrobial-resistant bacteria has become a serious problem in many countries. The aim of this

Original article PDF Seong Geun Hong1, Jongwook Lee2, Dongeun Yong3, Eui-Chong Kim4, Seok Hoon Jeong5, Yeon Jun Park6, Tae Yeal Choi7, Young Uh8, Jong Hee Shin9, Wee Kyo Lee10, Ji Young Ahn11, Sung-Hee Lee12, Gun-Jo Woo13, and Kyungwon Lee3 Departments of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1; Keonyang Univ. Med. Coll.2; Yonsei Univ. Coll. of Med.3; Seoul Nat. Univ. Coll. of Med.4; Coll. of Med. Kosin Univ.5; The Catholic Univ. of Kor.6; Hanyang Univ. Coll. of Med.7; Yonsei Univ. Wonju Coll. of Med.8; Chonnam Nat. Univ. Med. School9; Ajou Univ. Coll. of Med.10; Sooncheonhyang Univ. Coll. of Med.11; Cheju Hanmaeum Hospital12; Food Microbiology Division, Center for Food Safety Evaluation, Korea Food and Drug Administration13 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2004;7(2):171-177.Copyright © Korean Society of Clinical Microbiology. Abstract Background : A rapid increase in antimicrobial-resistant bacteria has become a serious problem in Korea. Moreover, the

Original article PDF Kyung Ran Jun, Hong Seon Jeon, Heungsup Sung, Mi-Na Kim Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2006;9(1):58-63.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the BD Phoenix Automated Microbiology System (Phoenix) for its ability to detect methicillin resistant Staphylococcus aureus(MRSA) and compared the results to those obtained by the Clinical and Laboratory Standards Institute (CLSI) agar dilution method, a mecA gene PCR method, and the MicroScan WalkAway 96 System (MicroScan). Methods: One hundred seventy S. aureus strains (Group I) isolated from blood and urine cultures were collected from eight university hospitals and 58 strains (Group II) including 20 blood isolates among Group I and 38 isolates from skin lesions of atopic patients were collected from Asan Medical Center. All 208 isolates were tested with Phoenix using PMIC/ID-53 panels, and the

Original article PDF Jung Oak Kang1, Eui Chong Kim2, Kyu Man Lee3, Nam Yong Lee4, Chang Kyu Lee5 Department of Laboratory Medicine, 1Hanyang University Guri Hospital, 2Seoul National University Hospital, 3Hallym University Hangang Sacred Heart Hospital, 4Sungkyunkwan University Samsung Medical Center, 5Korea University Guro Hospital, Seoul, Korea Corresponding to Kyu Man Lee, E-mail: kml@hallym.or.kr Ann Clin Microbiol 2007;10(2):102-108.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Respiratory viruses (RV) are important pathogen in both children and immunocompromised hosts. Rapid diagnosis of RV is important to manage patients and to implement infection control measures. To investigate the testing situation in Korea, we performed surveillance for the 95 medical institutions. Due to the paucity of long-term, multi-center data on RV epidemiology in Korea, we analyzed data from 5 university hospitals. Methods: Surveillance questionnaires were sent to 95 members of the Korean Society for Clinical Microbiology. The RV data from 5 university hospitals, 2001 through 2005, were collected

Original article PDF Hyukmin Lee1, Chang Ki Kim2, Jongwook Lee3, Sung-Hee Lee4, Ji Young Ahn5, Seong Geun Hong6, Yeon Jun Park7, Seok Hoon Jeong8, Eui-Chong Kim9, Wee Kyo Lee10, Young Uh11, Jong Hee Shin12, Tae Yeal Choi13, Hyo-Sun Kwak14, Kyungwon Lee2 Department of Laboratory Medicine, 1Kwandong University College of Medicine, Goyang; 2Yonsei University College of Medicine, Seoul; 3Keonyang University Medical College, Daejeon; 4Cheju Hanmaeum Hospital, Jeju; 5Sooncheonhyang University College of Medicine, Gumi; 6Pochon CHA University College of Medicine, Seongnam; 7 The Catholic University of Korea College of Medicine, Seoul; 8Kosin University College of Medicine, Busan; 9Seoul National University College of Medicine, Seoul; 10Ajou University College of Medicine, Suwon; 11Yonsei University Wonju College of Medicine, Wonju; 12Chonnam National Univeristy Medical School, Gwangju; 13Hanyang University College of Medicine, Seoul; 14Food Microbiology Division, Center for Food Safety Evaluation, Korea Food and Drug Administration, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2007;10(1):59-69.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Emergence and spread of antimicrobial

Original article PDF Sollip Kim1, Heungsup Sung1, Hong Sun Jeon1, Suk Ja Park1, Sang-Hyuk Park2, Mi-Na Kim1 1Department of Laboratory Medicine, Univertisy of Ulsan College of Medicine and Asan Medical Center, 2University of Ulsan College of Medicine, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2007;10(1):44-48.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Rapid and accurate surveillance is crucial in controlling vancomycin-resistant enterococci (VRE). Culture-based surveillance takes more than 4 days and direct polymerase chain reaction (PCR) is rapid but compromised by a low sensitivity. In this study, we evaluated the performance of an enrichment-PCR method for vanA VRE surveillance. Methods: In July 2006, 100 fecal specimens were inoculated to Enterococcosel agar (EA) and Enterococcosel broth (EB) containing 6μg/mL vancomycin. After 1 or 2 day-incubation bacterial pellets were obtained from 1 mL of blackened EB and VanA PCR were performed with DNA extract of the pellets (EB+PCR). Blackened EB were also

Original article PDF Kyoung Ho Roh1, Chang-Ki Kim2, Jong Hwa Yum3, Dongeun Yong4,5, Seok Hoon Jeong4,5, Chae Seung Lim1, Chang Kyu Lee1, Yunjung Cho1, Kyungwon Lee4,5, Yunsop Chong4,5 1Department of Laboratory Medicine, Korea University College of Medicine, 2Korean Institute of Tuberculosis, Seoul, 3Department of Clinical Laboratory Science, Dongeui University, Busan, 4Department of Laboratory Medicine and 5Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yuhs.ac Ann Clin Microbiol 2010;13(1):27-33. https://doi.org/10.5145/KJCM.2010.13.1.27Copyright © Korean Society of Clinical Microbiology. Abstract Background: Increasing numbers of Acinetobacter spp. resistant to multiple drugs, including carbapenem, has been a serious problem. The aims of this study were to determine carbapenem resistance patterns and mechanisms, as well as to study the molecular epidemiology of Acinetobacter spp. Methods: Clinical isolates of Acinetobacter spp. were collected from May to November in 2006. Antimicrobial susceptibility testing was performed using CLSI disk diffusion and