Original article PDF Dongeun Yong, M.D.,* Hyuk Min Lee, M.D.,* Jong Ha Ryu, M.D.,* Kyung Ho Roh, M.D.,* Won Ho Kim, M.D.,**,*** Kyungwon Lee, M.D.,*,*** and Yunsop Chong, M.D. Department of Laboratory Medicine, Research Institute of Bacterial Resistance,* Department of Internal Medicine,** and Brain Korea 21 Project for Medical Sciences,*** Yonsei University College of Medicine, Seoul, Korea Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2002;5(2):124-128.Copyright © Korean Society of Clinical Microbiology. Abstract Background: C. difficile-associated diarrhea (CDAD), the most frequently identified cause of nosocomial diarrhea, results from the overgrowth of cytotoxin (toxin B)-producing strains. The aim of this study was to evaluate the quantitative culture of Clostridium difficile to improve the laboratory diagnosis of CDAD. Methods: The quantitative culture and cytotoxin gene results were evaluated based on the findings of colonoscopy and/or histology of the biopsy specimens. Results: Among the 402 specimens with cytotoxin-positive isolates, 301 (74.9%) contained ≥106 CFU/mL of C. difficile.

Original article PDF Jongwook Lee, Pum Soo Kim*, Kyungwon Lee**, Jae Hag Lee***, Jae Kyoung Lee****, Chang Hoon Lee***** Department of Laboratory Medicine, Coll. Of Med, Konyang Univ., Daejon, Department of Internal Medicine, Coll. Of Med.,Inha Univ.*, Incheon, Department of Laboratory Medicine, Yonsei Univ. Coll. Of Med.**, Department of food and Nutrition, Seoil College***, Jongang University****, KonKuk Univ. Coll. Of Med*****, Seoul, Korea Corresponding to Pum Soo Kim, E-mail: pskim@inha.ac.kr Ann Clin Microbiol 2003;6(2):156-159.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Helicobacter pylori is the single most common pathogen that causes chronic bacterial infection in human. The authors designed a new type of urease detection method (Asan Helicobacter test) that can be used for rapid early detection of H. pylori as well as a transport medium. This medium has a strong acidity with a minimal concentration of urea for the purpose of the detection of H. pylori. The current study was to evaluate the

Original article PDF Seong Geun Hong1, Sunjoo Kim2, Seok Hoon Jeong3, Chulhun L. Chang4, Sung Ran Cho5, Ji Young Ahn5, Jong Hee Shin6, Hye Soo Lee7, Won Keun Song8, Young Uh9, Jong Hwa Yum10, Dongeun Yong10, Kyungwon Lee10, Yunsop Chong10 Department of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Gyeongsang Nat. Univ. School of Med.2, Coll. of Med. Kosin Univ.3, Pusan Nat. Univ. Coll. of Med.4, Sooncheonhyang Univ. Coll. of Med.5, Chonnam Nat. Univ. Med. School6, Chonbuk Univ. Med. School7, Hanllym Univ. Coll. of Med.8, Yonsei Univ. Wonju Coll. of Med.9, Yonsei Univ. Coll. of Med.10 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(2):149-155.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Increase in extended-spectrum β-lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae have been reported in Korea. The aim of this study was to determine the nationwide prevalence of ESBL-producing E. coli and K. pneumoniae, and to investigate the types of ESBLs. Methods：A total of 2,221 E. coli and

Original article PDF Wee Gyo Lee, Suck Ho Lee*, Sun Min Lee**, Ji Young Huh, Dongeun Yong***, Kyungwon Lee***, Wan Shik Shin**** and Dong Gun Lee**** Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Division of Gastroenterology*, Department of Internal Medicine, Sooncheonhyang University Cheonan Hospital, Cheonan, Department of Laboratory Medicine, Bestian Medical Center**, Yonsei University School of Medicine***, Division of Infectious Disease****, Department of Internal Medicine, Catholic University, Seoul, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann Clin Microbiol 2003;6(2):119-125.Copyright © Korean Society of Clinical Microbiology. Abstract Background : The widespread dissemination of Tn1546 has been attributed to transposition into plasmid or transferable elements. The transposition has been achieved through the activity of insertion sequences. Genetic diversity in Tn1546 includes integration of IS elements such as IS1216V, IS1251, IS1476 and IS1542. We investigated molecular typing and the distribution of insertion sequences in vanA-containing Enterococcus faecium isolated from patients in a teaching hospital. Methods

Original article PDF Ji Young Huh, Sun Min Lee*, Suck Ho Lee**, Wee Gyo Lee, Dongeun Yong***, Kyungwon Lee***, Wan Shik Shin**** and Dong Gun Lee**** Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Bestian Medical Center*, Seoul, Division of Gastroenterology**, Department of Internal Medicine, Sooncheonhyang University Cheonan Hospital, Cheonan, Department o f Laboratory Medicine***, Yonsei University School of Medicine, Division of Infectious Disease****, Department of Internal Medicine, Catholic University, Seoul, Korea Corresponding to Ji Young Huh, E-mail: heidi2030@hanmail.net Ann Clin Microbiol 2003;6(2):114-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Vancomycin-resistant enterococci (VRE) have been increasingly isolated worldwide as a nosocomial pathogen. In Korea, because avoparcin has been used as a growth promoter in animal feed, vanA-containing enterococci have been found in animals. The aim of this study is to understand the epidimiology of VRE isolated from chicken of diverse geographic areas.  Methods : Thirty eight isolates

Original article PDF Hee Kyung Park, Young Mi Lee, Hyun Jung Jang, Cheol Min Kim, Kyungwon Lee, Seok Hoon Jeong, Mooin Park Institute for Biomedical Research, SJ Hightech Co.; Department of Biochemistry*, Pusan National University College of Medicine, Busan; Department of Diagnostic Medicine** and Research Institute of Bacterial Resistance***, Yonsei University College of Medicine, Seoul; Department of Diagnostic Medicine**** and Internal Medicine*****, Kosin University College of Medicine, Busan, Korea Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr Ann Clin Microbiol 2003;6(1):63-68.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Clostridium difficile is the major cause of antibiotic-associated diarrhea (AAD) and pseudomembranous colitis (PMC). The aim of this study was to develop the nested PCR assay for direct detection of toxigenic C. difficile in stool specimens and to evaluate the usefulness of the method. Methods: Specificity of newly designed primers are tested with 36 reference strains of intestinal flora. Lower detection limit of nested PCR for

Original article PDF Seong Geun Hong1, Dongeun Yong2, Kyungwon Lee2, Eui-Chong Kim3, Wee Kyo Lee4, Seok Hoon Jeong5, Won Keun Song6, Yeon Jun Park7, Mi-Na Kim8, Young Uh9, Jong Hee Shin10, Jongwook Lee11, Ji Young Ahn12, Sun Wha Lee13, Jae Seok Kim5, Hee Bong Shin1 Departments of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Yonsei Univ. Coll. of Med.2 Seoul Nat. Univ. Coll. of Med.3, Ajou Univ. of Med.4, Coll. of Med. Kosin Univ.5, Hanllym Univ. Coll. of Med.6 The Catholic Univ. of Kor.7, Univ. of Ulsan Med. Coll. and Asan Med. Center8, Yonsei Univ. Wonju Coll. of Med.9 Chonnam Nat. Univ. Med. School10, Keonyang Univ. Med. Coll.11 Sooncheonhyang Univ. Coll. of Med.12; Neodin Med. Res. Center13 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(1):29-36.Copyright © Korean Society of Clinical Microbiology. Abstract Background: A rapid increase of antimicrobial-resistant bacteria has become a serious problem in many countries. The aim of this

Original article PDF Seong Geun Hong1, Jongwook Lee2, Dongeun Yong3, Eui-Chong Kim4, Seok Hoon Jeong5, Yeon Jun Park6, Tae Yeal Choi7, Young Uh8, Jong Hee Shin9, Wee Kyo Lee10, Ji Young Ahn11, Sung-Hee Lee12, Gun-Jo Woo13, and Kyungwon Lee3 Departments of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1; Keonyang Univ. Med. Coll.2; Yonsei Univ. Coll. of Med.3; Seoul Nat. Univ. Coll. of Med.4; Coll. of Med. Kosin Univ.5; The Catholic Univ. of Kor.6; Hanyang Univ. Coll. of Med.7; Yonsei Univ. Wonju Coll. of Med.8; Chonnam Nat. Univ. Med. School9; Ajou Univ. Coll. of Med.10; Sooncheonhyang Univ. Coll. of Med.11; Cheju Hanmaeum Hospital12; Food Microbiology Division, Center for Food Safety Evaluation, Korea Food and Drug Administration13 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2004;7(2):171-177.Copyright © Korean Society of Clinical Microbiology. Abstract Background : A rapid increase in antimicrobial-resistant bacteria has become a serious problem in Korea. Moreover, the

Original article PDF Byung-Chan Jeon2, Ki-Young Kwon2, Seok Hoon Jeong1, Il Kwon Bae1, Su Bong Kwon1, Byung Kyu Cho1, Dongeun Yong3, Kyungwon Lee3 Departments of Laboratory Medicine1, Neurosurgery2, Kosin University College of Medicine; Department of Laboratory Medicine3, Yonsei University College of Medicine Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr Ann Clin Microbiol 2004;7(2):139-147.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Acinetobacter baumannii is a glucose-nonfermenting gram-negative rod and is a well-recognized nosocomial pathogen. In recent years, A. baumannii strains showing resistance to carbapenems by producing metallo-β-lactamases or OXA-type β-lactamases have increased, and it is considered to be a serious clinical problem. But genotypes of carbapenemases produced by A. baumannii isolates in Korea have been rarely reported. The purpose of this study was to investigate the prevalence of imipenem-resistant A. baumannii and to determine the mechanism of resistance. Methods : During the period of January through September, 2003, susceptibilities to imipenem of A. baumannii isolates from patients admitted in Kosin

Original article PDF Jung Hyun Lee1, Il Kwon Bae2, Su Bong Kwon2, Seok Hoon Jeong2,Gun Jo Woo3, Jongwook Lee4, Wee Gyo Lee5, Jung Oak Kang6, Ji Young Ahn7, Seong Geun Hong8, Jong Hee Shin9, Young Uh10, Yeon Jun Park11, Eui-Chong Kim12, Kyungwon Lee13, Dongeun Yong13 Departments of Pediatrics1 and Laboratory Medicine2, Kosin University College of Medicine; Division of Food Microbiology, Korea Food and Drug Administration3; Departments of Laboratory Medicine, Keonyang University College of Medicine4, Ajou University College of Medicine5, Hanyang University College of Medicine6, Sooncheonhyang University College of Medicine7, Pochon CHA University College of Medicine8, Chonnam National University Medical school9 , Yonsei University Wonju College of Medicine10, The Catholic University of Korea College of Medicine11, Seoul National University College of Medicine12, Yonsei University College of Medicine13 Corresponding to Seok Hoon Jeong, E-mail: kscpjsh@ns.kosinmed.or.kr Ann Clin Microbiol 2004;7(2):111-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background : The aims of this study were