Original article PDF Yoon Hee Kang, M.D., Soo Jin Choi, M.D., Sang Hyun Hwang, M.D., Young Wook Cho, M.D., Duck-Hee Kim, M.T.*, Mi-Na Kim, M.D. and Chik Hyun Pai, M.D. Department of Clinical Pathology, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim Ann Clin Microbiol 1999;2(2):158-166.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Escherichia coli and Klebsiella pneumoniae resistant to 3rd generation cephalosporin have been reported with increasing frequency in tertiary-care hospital in Korea. MicroScan Neg Combo Panel Type 21 (Type 21) contains a 1 µg/mL cepfoxodime (POD) in addition to other screen wells containing ceftazidime, cefotaxime, ceftriaxone, and aztreonam, which are designed for detecting extended-spectrum β-lactamase (ESBL)-producing E. coli and Klebsiella species. We evaluated the Type 21 panel for its ability to detect ESBL. Methods: From November to December in 1998, 496 E. coli and 326 K. pneumoniae strains isolated from clinical specimens were tested with Type 21 panel. The isolates

Original article PDF Young Uh, Jeong Seog Son, Gyu Yel Hwang, In Ho Jang, Kap Jun Yoon and Dong Min Seo* Departments of Clinical Pathology and Medical Information Development*, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 1999;2(2):135-143.Copyright © Korean Society of Clinical Microbiology. Abstract Background: To access the accuracy and clinical usefulness of microplate identification (ID) system for the identification of Enterobacteriaceae, we compared microplate ID system with API 20E (bioMérieux, Marcy I’Etoile, France) Methods: Ninety-two cultures of Enterobacteriaceae and one isolate of Aeromonas species were simultaneously identified by microplate ID system and the API 20E. Twenty biochemical tests used in microplate ID system were lactose, sucrose, and H₂S in Kigler’s iron agar media; indole, sucrose, raffinose, arabinose, trehalose, adonitol, dulcitol, sorbitol, cellibiose, methy-red, phenylalanine deaminase, ornithine decarboxylase, lysine decarboxylase, arginine dihydrolase, urease, and citrate in microplate; and oxidase test. The identification was obtained by considering percent likelihood

Original article PDF Young Uh*,** Gyu Yel Hwang*, In Ho Jang*, Jong Sun Park*, Oh-Gun Kwon*, and Kap Jun Yoon* Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju*; Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea** Corresponding to Young Uh Ann Clin Microbiol 1999;2(2):131-134.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The erythromycin-resistance rate and phenotype distribution of Streptococcus pyogenes are quite different by geographical variation and study period. The aim of the present study was to determine the evolution of resistance to erythromycin and the frequency of erythromycin resistance phenotype of S. pyogenes isolated from Wonju Christian Hospital. Methods: The minimal inhibitory concentrations (MICs) of erythromycin and clindamycin for 94 S. pyogenes isolated from clinical specimens between 1990 to 1998 were investigated. Double disk test of erythromycin (78µg) and clindamycin (25µg) were performed for 15 isolates of erythromycin-resistant S. pyogenes to evaluate the erythromycin resistance phenotype. Results: The resistance

Original article PDF Young Uh, In Ho Jang, Gyu Yel Hwang, Kap Jun Yoon and Hyung-Hoan Lee* Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea, and Department of Biology, Kon-Kuk University, Seoul, Korea* Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 1999;2(1):58-63.Copyright © Korean Society of Clinical Microbiology. Abstract Background：The accurate and rapid identification of enterococci can provide clinician’s decision making of antimicrobial therapy because enterococci are usually multiresistant to commonly used antimicrobial agents and antimicrobial resistance patterns are different according to enterococcal species. Accuracy of identification system depends mainly on data base such as positive rate of biochemical reactions, relative frequency of occurrence of biotype, and isolation frequency of microorganisms. The purpose of this study was to analyze the isolation rate and biotype frequency of enterococci isolated from clinical specimens. Methods：We used a simplified identification system for the identification of the enterococci from clinical

Original article PDF Byung Lip Kim*, M.D., Seok Hoon Jeong, M.D., Ja Young Koo*, M.D., Kyungwon Lee**, M.D., Yunsop Chong**, Ph.D., Tae Jeon Jeong, M.T., Hyun Yong Hwang, M.D. and Mi Hyang Kim, M.D. Department of Internal Medicine* and Clinical Pathology, College of Medicine Kosin University, Pusan, and Department of Clinical Pathology, College of Medicine Yonsei University, Seoul, Korea** Corresponding to Seok Hoon Jeong Ann Clin Microbiol 1999;2(1):28-39.Copyright © Korean Society of Clinical Microbiology. Abstract Background ： Increased isolation of extended-spectrum β-lactamase ( ESBL)-producing Enterobacteriaceae resistant to third generation cephalosporins and aztreonam has been noted recently. This study was to determine the prevalence of resistance to these drugs and ESBL in Enterobacteriaceae and to evaluate the methods for detection. Methods：During the period of October, 1997 and March, 1998, a total of 731 clinical isolates of Enterobacteriaceae were collected from patients of the Kosin Medical Center, Pusan, Korea. Antimicrobial susceptibility test by disk diffusion method and double disk synergy

Original article PDF Chulhun L. Chang, M.D., Dae Young Seo, M.D.,* Tae Hee Park, M.D., Jeong Seon Park, M.D., and Weon Joo Hwang, M.T. Department of Clinical Pathology, Pusan National University Hospital, Pusan; Department of Clinical Pathology,* Dong-A University Hospital, Pusan, Korea Corresponding to Chulhun L. Chang, E-mail: cchl@hyowon.cc.pusan.ac.kr Ann Clin Microbiol 2001;4(1):40-44.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Mycobacterial false-positive cultures have rarely been recognized in Korea, even though the rate of false-positive cultures of Mycobaterium tuberculosis has ranged from 0.4% to 4.0%. We estimated the false-positive rates by the review of medical records from whom mycobacterial cultures were requested, retrospeaively, after a bout of false-positive cultures was discovered in specimens treated in a single day. Methods : Of the total 2,245 specimens, including 337 positive cultures of mycobacteria, during the period of January and June 1999, seventy-two specimens that showed colonies less than or equal to 5 colonies

Original article PDF Young Uh, Gyu Yel Hwang, In Ho Jang, Hyeun Gyeo Lee, An Suk Jeoung, Soon Deok Park, Jeong Seog Son, and Kap Jun Yoon Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2001;4(1):22-27.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Although most of aerobic gram-positive bacilli have been considered to be contaminants, gram-positive bacilli should be identified to the species level if they are isolated from sterile body sites such as blood, and from adequately collected clinical specimens if they are the predominant organisms. However, identification of gram-positive bacilli are difficult due to the enormous diversity of these organisms and the small number of readily available commercial identification systems in clinical laboratories. Gram-positive bacilli and coccorods isolated from blood cultures were tested with BBL Crystal Gram-Positive (GP) Identification (ID) system in order to

Original article PDF Hee Young Chung, M.D.1, Yoo Li Kim, Ph.D.2, Kyung Ah Hwang, Ph.D.2, Byung Hoo Choi, M.T.1, Sook Ja Park, M.T.1, Heung Sup Sung, M.D.1, Mi-Na Kim, M.D.1 1Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, 2 BioSewoom Institutue Bioscience and Biotechnology, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2007;10(2):77-83.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the performance of a newly developed real-time polymerase chain reaction (PCR) method using TaqMan probe (TP) and internal control (IC) for quantitation of BK virus (BKV) DNA. Methods: PCR primers and TP were targeted for the VP1 of BKV and 300 bp-region of VP1 was cloned to prepare a standard DNA. Threshold cycles (Ct) of IC was set at 33±3. The recovery rates, precision, linearity, and limit of detection (LOD) were measured using the standard DNA. To correlate TP with previous

Case report PDF Gyoung Yim Ha1, Young Sil Choi2, Moon Yeon Kim1, Young Hyun Lee3, Kyoung Seop Lee4, Kyu Jam Hwang2, Mi Yeon Pak2 Departments of 1Laboratory Medicine, 3Internal Medicine, and 4Urology, College of Medicine, Dongguk University, Gyeongju; 2Division of Zoonoses, Center for Immunology and Pathology, National Institute of Health, Seoul, Korea Corresponding to Gyoung Yim Ha, E-mail: doctor61gyha@hanmail.net Ann Clin Microbiol 2007;10(2):154-159.Copyright © Korean Society of Clinical Microbiology. Abstract Brucellosis is a zoonosis caused by Brucella species. B. melitensis, B. suis, B. abortus and B. canis can infect humans. Recently, as the cases of bovine brucellosis have increased every year in Korea, the cases of human brucellosis have also increased among livestock workers and veterinarians in rural areas, since the first human case was reported in 2003. Because clinical manifestations of the disease are nonspecific and may be very atypical, clinicians and laboratory persons need to be active in using diagnostic tools including polymerase chain reaction in addition to the ordinary

Original article PDF Young Uh, Gyu Yul Hwang, Kap Jun Yoon, Hyo Youl Kim1, Hong Sun Uh2, O Kab Kwon3 Departments of Laboratory Medicine and 1Infectious Diseases, Yonsei University Wonju College of Medicine, Wonju; 2Uh Urologic Clinic, 3Nepes Corporation, Seoul, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2007;10(1):37-43.Copyright © Korean Society of Clinical Microbiology. Abstract Background: It is well-known that silver ions and silver compounds are broad-spectrum antimicrobial agents effective against gram-positive and gram-negative bacteria, and yeasts. Thus, silver ions, as an antibacterial agent, have been used in the components of materials used in medical devices or coatings. Recently, advances in nanotechnology have enabled manufacturers to develop silver particles of a nanometer size with a safer and more effective antimicrobial activity. So, we evaluate the antimicrobial activity of nanoSilver-coated gauze against clinical isolates.  Methods: Three kinds of nanoSilver-coated gauzes (100Å, 800Å, and 1,500Å) were tested for antimicrobial activity by the