Original article PDF Young Uh*, Soon Deok Park, Jeong Seog Son*, Hyeun Gyeo Lee*, An Suk Jeoung*, Hyun Mi Cho*, and Kap Jun Yoon*, and Hyo Youl Kim Departments of Clinical Pathology* and Internal Medicine**, Yonsei University Wonju College of Medicine, Wonju, and Research Institute of Bacterial Resistance***, Yonsei University College of Medicine, Seoul, Korea Corresponding to Young Uh Ann Clin Microbiol 2000;3(2):99-110.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In recent years, the incidence of extended-spectrum β-lactamase (ESBL) producing Klebsiella has been steadily increased, and the newer species K. planticola and K. terrigena, formerly regarded as nonpathogen, have been reported with astonishing frequency from human infectious processes by some investigators. The aim of this study is to elucidate the isolation rate and antimicrobial susceptibility of recent clinical Klebsiella isolates. Method: For the clinical Klebsiella isolates during the period of June 1999 to May 2000, isolation frequency of Klebsiella species by specimen, departments, age, and sex were analyzed. And antimicrobial susceptibilities

Original article PDF Oh-Gun Kwon, M.D., Hyun Mi Cho, M.T., In Ho Jang, M.S., Young Uh, M.D., and Kap Jun Yoon, M.D. Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 2000;3(2):116-120.Copyright © Korean Society of Clinical Microbiology. Abstract Background: As many as several weeks of incubation may be necessary for the recovery of mycobacteria when conventional culture media are used. Previous studies evaluating Mycobacteria Growth Indicator Tube (MGIT) as a rapid for the growth and detection of mycobacteria from clinical specimens have been reported. We compared MGIT with Ogawa media for the recovery of mycobacteria from clinical specimens. Methods: Ninety nine clinical specimens received in the laboratory of Wonju Christian Hospital from June to September 1999 were used for this study. The specimens from nonsterile body sites were digested, decontaminated, and concentrated for culture and Ziehl-Neelsen stain, and specimen

Original article PDF Young Uh, M.D., In Ho Jang, M.D., Gyu Yel Hwang, M.D., Mi Kyung Lee, M.D. and Kap Jun Yoon, M.D. Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 2000;3(1):30-35.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Pigment production and acidification of ribose are most frequently used biochemical tests for the differentiation of three enterococcal species carrying vanC genes such as Enterococcus gallinarum, Enterococcus casseliflavus, and Enterococcus flavescens. However, pigment production may occasionally be negative in E. casseliflavus, and some of E. casseliflavus may be negative or delayed reaction with ribose fermentation test. So, we performed this study to find out biochemical tests capable of distinguishing the strains possessing vanC genotypes. Methods: A total of 17 enterococci composed of 14 clinical isolates with motility or pigment positive strains and three ATCC strains (E. gallinarum ATCC 49573, E. casseliflavus ATCC 25788, and E. flavescens ATCC 49997) were tested by multiplex PCR of the vanC genes (vanC-1, vanC-2, and vanC-3) and various

Case report PDF Young Uh, Hyeun Gyeo Lee, Gyu Yel Hwang, Kap Jun Yoon, and Hyo Youl Kim* Departments of Clinical Pathology and Internal Medicine*, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2001;4(2):146-149.Copyright © Korean Society of Clinical Microbiology. Abstract Although Enterococcus casseliflavus with intrinsic low-level vancomycin resistance has rarely been isolated from clinical specimens, this organism may cause serious invasive infections such as endocarditis and bacteremia. This low prevalence may be due, in part, to the inability of automated systems to recognize this organism. Vancomycin may not be effective against E. casseliflavus, despite in vitro results that indicate vancomycin susceptibility. It is important that all E. casseliflavus isolates obtained from clinical specimens that are related to serious infections should be identified to species level for appropriate antibiotic therapy. We report a case of bacteremia caused by E. casseliflavus in a 44-year-old female patient with liver disease.

Case report PDF Hyun Mi Cho, Young Uh, Kap Jun Yoon, Won Yeon Lee*, and Hyo Youl Kim* Departments of Clinical Pathology and Internal Medicine*, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wanju.yonsei.ac.kr Ann Clin Microbiol 2001;4(2):142-145.Copyright © Korean Society of Clinical Microbiology. Abstract Plesiomonas shigelloides is an oxidase-positive, fermentative, gram-negative rod currently classified as a member of the family Vibrionaceae. P. shigelloides has been implicated as the causative agents of gastroenteritis as well as extraintestinal infections such as septicemia, neonatal meningitis, cellulitis, and cholecystitis. Septicemia due to P. shigelloides is very rare but is severe and has been associated with a high mortality rate. We report a case of septicemia caused by P. shigelloides in a 66-year-old male with diabetes mellitus who had diagnosed as liver cirrhosis 7 years before. (Korean J Clin Microbiol 2001;4(2):142-145) Keywords Plesiomonas shigelloides, Septicemia, Extraintestinal infection

Original article PDF Gyu Yel Hwang, Young Uh, Hyun-Joo Kim, In Ho Jang, and Kap Jun Yoon Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 2001;4(1):62-66.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Accurate detection of extended spectrum β-lactamase (ESBL) is important because ESBLs producing organisms may appear susceptible to oxyimino-β-lactams in standard susceptibility tests, but are considered to be clinically resistant to these drugs. Conventional antimicrobial susceptibility test methods do not reliably detect ESBL production. Molecular techniques and NCCLS broth dilution method, which detect ESBL production, may be time consuming, expensive and technically difficult to perform. The purpose of this study is to evaluate the clinical usefulness of NCCLS ESBL phenotypic confirmatory test by disk diffusion method. Methods : For 96 Escherichia coli and 49 Klebsiella pneumoniae isolates collected between December 2000 to February 2001, double disk synergy test, NCCLS

Original article PDF Young Uh, M.D., In Ho Jang, M.S., Kap Jun Yoon, M.D., Hyun Soo Kim*, M.D., and Hyo Youl Kim*, M.D. Departments of Clinical Pathology and Internal Medicine*, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh Ann Clin Microbiol 2001;4(1):28-32.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Vibrio species may be classified as halophilic or nonhalophilic on the basis of their requirement of NaCl for optimal growth. Recently, attention has been focused on the halophilic vibrios and Vibrio cholerae non-O1/O139 causing extraintestinal infections such as septicemia. The aim of this study is to elucidate the isolation rate and clinical manifestations of Vibrio species isolated from clinical specimens between 1996 and 2000 at Wonju Christian Hospital. Methods : Stool specimens were inoculated onto the thiosulfate-citrate-bile salt-sucrose media, blood cultures were performed by automated blood culture systems with commercial bottles, and the others were cultured according to the routine procedures. Results :

Original article PDF Young Uh, Gyu Yel Hwang, In Ho Jang, Hyeun Gyeo Lee, An Suk Jeoung, Soon Deok Park, Jeong Seog Son, and Kap Jun Yoon Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2001;4(1):22-27.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Although most of aerobic gram-positive bacilli have been considered to be contaminants, gram-positive bacilli should be identified to the species level if they are isolated from sterile body sites such as blood, and from adequately collected clinical specimens if they are the predominant organisms. However, identification of gram-positive bacilli are difficult due to the enormous diversity of these organisms and the small number of readily available commercial identification systems in clinical laboratories. Gram-positive bacilli and coccorods isolated from blood cultures were tested with BBL Crystal Gram-Positive (GP) Identification (ID) system in order to

Original article PDF Kap Jun Yoon, M.D., Young Uh, M.D., Gyu Yel Hwang, M.S., In Ho Jang, M.S., Mi Kyung Lee, M.T. Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Kap Jun Yoon Ann Clin Microbiol 2001;4(1):16-21.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Macrolide resistance in β-hemolytic streptococci has increased during the 1990s, and the proportion of MLS (Macrolide-lincosamide-streptogramin) resistance phenotypes and genotypes of β-hemolytic streptococci are quite different by geographical variation and study period. The aim of the present study was to determine the distribution of MLS resistance phenotypes and genotypes in β-hemolytic streptococci isolated from Wonju Christian Hospital. Methods : The minimal inhibitory concentrations of erythromycin and clindamycin of 426 β-hemolytic streptococci isolated from clinical specimens between 1990 to 1999 were determined by agar dilution method. MLS resistance phenotypes were determined by double disk diffusion method using erythromycin and clindamycin disk, and genotypes were

Case report PDF Young Uh, Hyeun Gyeo Lee, Gyu Yel Hwang, Kap Jun Yoon, Soo Young Yoo*, Baik Soon Koo**, and Hyo Youl Kim** Departments of Clinical Pathology, General Surgery*, and Internal Medicine**, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2002;5(1):62-67.Copyright © Korean Society of Clinical Microbiology. Abstract Although Leuconostoc species with intrinsic high-level vancomycin resistance have rarely been isolated from clinical specimens, this organism may cause serious invasive infections such as bacteremia and meningitis in immunocompromised hosts or patients with a wide spectrum of underlying diseases including alcoholic liver diseases and gastrointestinal diseases. Predisposing factors of Leuconostoc bacteremia include intravenous or enteral feeding catheters, other invasive access devices such as tracheostomy, gastrostomy or endotracheal tubes, and previous antimicrobial treatment. This low prevalence may be due, in part, to the inability of automated systems to recognize this organism. It is important that all Leuconostoc isolates obtained